Role of methylation-related genes CRYAB and SLC39A11 in the occurrence and development of lung adenocarcinoma

Abstract

Background: Previous studies have shown that human crystallin alpha B (CRYAB) is highly expressed in human cancers and associated with poor survival in cancer patients. Here we investigated whether SLC39A11 and CRYAB genes were related to the proliferation and development of lung adenocarcinoma (LUAD) to explore their potential as therapeutic targets and prognostic markers for LUAD. Methods: CRYAB and SLC39A11 genes were identified from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database. The human lung cancer cell lines A549 and H1975 were cultured, transfected, and subjected to RNA extraction. After genomic DNA removal, the RNA was reversetranscribed. Differences between 2 groups were compared using t-test.Results: Knockdown of SLC39A11 inhibited the proliferation of LUAD cells in A549 and H1975.Knockdown of CRYAB promoted the increase of LUAD cell clones, while knockdown of SLC39A11 suppressed LUAD cell clones. In both A549 and H1975 cell lines, knockout of CRYAB inhibited the apoptosis of LUAD cells, whereas knockout of SLC39A11 promoted the apoptosis of LUAD cells. In the H1975 cell line, knockout of CRYAB also lowered the proportion of cells in interphase and increased the proportion of mitotic cells, while knockout of SLC39A11 also slowed down the division cycle of tumor cells.Knockdown of CRYAB promoted the migration of LUAD cells in both the A549 cell line and H1975 cell line. In the H1975 cell line, knockout of SLC39A11 also reduced the invasive ability of LUAD cells.Conclusions: CRYAB and SLC39A11 could be used as prognostic indicators and therapeutic targets for LUAD.

A zinc metabolism-related gene signature for predicting prognosis and characteristics of breast cancer

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A novel zinc metabolism-related gene signature to predict prognosis and immunotherapy response in lung adenocarcinoma

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Screening of Colon Cancer Apoptosis-Related Genes Based on Bioinformatics and Construction of Survival Prognostic Model

“…CRYAB is widely believed to function primarily as a molecular cocrystallin to promote cell survival. In addition to acting as a molecular chaperone, it has been shown to be involved in the occurrence of multiple tumors [18] [19]. However, CRYAB has been shown to inhibit the proliferation and metastasis of CRC cells [20].…”