Role of microRNA-4516 involved autophagy associated with exposure to fine particulate matter

Abstract

Metals are vital toxic components of fine particulate matter (PM2.5). Cellular responses to exposure to PM2.5 or PM metal components remain unknown. Post-transcriptional profiling and subsequent cell- and individual-based assays implied that the metal ion-binding miR-4516/RPL37/autophagy pathway could play a critical role in cellular responses to PM2.5 and PM metal stresses. miR-4516 was up-regulated in A549 cells exposed to PM2.5 and in the serum of individuals living in a city with moderate air pollution. The expression levels of the miR-4516 target genes, namely, RPL37 and UBA52, were involved in ribosome function and inhibited by exposure to PM2.5 and PM metal components. Autophagy in A549 cells was induced by PM2.5 exposure as a response to decreased RPL37 expression. Moreover, enhanced miR-4516 expression was positively correlated with the augmentation of the internal burden of aluminum and lead in individuals living in a city with moderate air pollution. Hereby, the miR-4516/RPL37/autophagy pathway may represent a novel mechanism that mediates responses to PM metal components.

MicroRNA Changes in Firefighters

“… 58 MiR-4516 expression was decreased in our incumbent firefighters, but was upregulated in a study of A549 cells exposed to PM 2.5 as well as the serum of persons living in a Chinese city with moderate air pollution. 59 MiR-145-5p expression was decreased in our incumbent firefighters, but increased in service members with polychlorinated dibenzodioxin (PCDD) and polychlorinated dibenzofuran (PCDF) exposures from open air burn pits comparing pre- and post-deployment. 60 However, to evaluate for dose–response relationships specific to firefighters, longitudinal studies including exposure assessment and measurement of miRNA changes are needed.…”

Exosomes derived from miR-140-5p-overexpressing human synovial mesenchymal stem cells enhance cartilage tissue regeneration and prevent osteoarthritis of the knee in a rat model

“…Key miRNAs are miR-6090, which may play a role in embryo development 62; miR-4516, which is involved in autophagy63; miR-3960, which is related to Runx264, and miR-4459, which is involved in altering the stemness of embryonic stem cells65. However, the relationship between these microRNAs and chondrocytes remains unknown and needs to be further researched.…”

Melatonin‐stimulated exosomes enhance the regenerative potential of chronic kidney disease‐derived mesenchymal stem/stromal cells via cellular prion proteins

“…Using miRNA profiling analysis, we have identified that melatonin increases the expression of hsa‐miR‐4516, a major player in the regulation of PrP C expression, in MSC‐derived exosomes. Some studies have shown that miR‐4516 is involved in autophagy and is a potential biomarker for dust‐induced pulmonary fibrosis . A recent study has revealed miR‐4516 as a novel therapeutic target against glioblastoma .…”