Role of microRNAs in antiviral responses to dengue infection

Wong, Rui Rui; Abd‐Aziz, Noraini; Affendi, Sarah; Poh, Chit Laa

doi:10.1186/s12929-019-0614-x

Cited by 75 publications

(55 citation statements)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Discussionmentioning

confidence: 99%

“…For example, MRX34, a microRNA (miRNA) mimic, has reached phase 1 studies in patients with advanced solid tumors, including hepatocellular carcinoma, pancreatic cancer and cholangiocarcinoma ( 22 ). miR-146a may serve as a promising therapeutic target for dengue ( 23 ). Wheatley et al ( 24 ) previously developed a DNA vaccine vector co-expressing an miRNA designed in silico miRNA to inhibit PERK with a HIV-1 envelope.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Circular RNA expression profiles significantly altered in UVA‑irradiated human dermal fibroblasts

Lin

Zheng

et al. 2020

Exp Ther Med

View full text Add to dashboard Cite

Circular RNAs (circRNAs) have been previously implicated in number of diseases. However, the roles of circRNAs in photoaging remain elusive. In the present study, to understand if photoaging influences the levels of circRNA expression, the expression of circRNAs in ultraviolet A (UVA)-irradiated human dermal fibroblasts were profiled. A total of 128 circRNAs were identified to be differentially expressed (fold change >1.5; P<0.05) after UVA exposure, including 39 upregulated and 89 downregulated circRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes biological pathway analyses indicated that the differentially expressed circRNAs were associated with extracellular matrix organization and metabolism. The present study revealed an altered circRNA expression pattern in human dermal fibroblasts following UVA-irradiation. These results provide not only a basis for in-depth study of the mechanism of skin photoaging but also a new possibility for the prevention and treatment of photoaging and associated skin diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Circular RNA expression profiles significantly altered in UVA‑irradiated human dermal fibroblasts

Lin

Zheng

et al. 2020

Exp Ther Med

View full text Add to dashboard Cite

show abstract

“…Of particular interest is the possibility that nucleotide sequences from the 5'UTR are crucial in enabling YFV to propagate in primates and/or mosquitoes. Although no experiments have thus far reported systematically investigating the constancy of nucleotides in the 5'UTR, the effectiveness of the varieties of small interfering RNA in the control of virus infection is a field of active investigation (21)(22)(23)(24)(25). Should suitable conditions be found in which 5'UTR nucleotide sequences are determined to be inhibitory to YFV, parenteral delivery methods of the relevant inhibitory nucleotides would need to be developed to achieve a therapeutic effect.…”

Section: Discussionmentioning

confidence: 99%

Constancy and Change in the 5’UTR of Yellow Fever Virus

Seligman

2020

Preprint

View full text Add to dashboard Cite

The nucleotide composition of the 5’UTR of the yellow fever virus (YFV) has been reported to be the most constant sequence in the viral genome, but a comprehensive analysis of this constancy has not been presented. The current report is an analysis of the 5’UTRs from 48 sequences deposited in GenBank representing the seven described genotypes, five in Africa and two in the Americas. The YFV 5’UTRs consist of 118-120 nucleotides, 92% (110/119) of which are constant in all sequences. The constancy is impressive and suggests that many participate in significant viral functions. Remarkably, analysis of the non-constant nucleotides revealed that in some instances the non-constant nucleotide changes persisted in one or a restricted number of related genotypes and were from sequences isolated over a considerable span of years. This constant feature of non-constant nucleotides is consistent with the concept that the changes were in response to different environmental features such as changes in mosquito hosts or animal reservoirs, particularly as a consequence of spread of YFV from Africa to the New World. Constancy of 5’UTR in general may be helpful in distinction of viral species. Lastly, the presence of sequences of constant nucleotides greater than 19 nucleotides suggests regions of the 5’UTR that may be exploited for use as non-codon RNA as treatment and diagnostic agents in a variety of viral diseases.ImportanceThe 5’UTR is arguably the most neglected portion of the viral genome. It is frequently incomplete in the sequences deposited as otherwise complete sequences in GenBank. The current report is an analysis of complete 5’UTR sequences selected from those deposited in GenBank and indicates that the 5’UTR is 92% conserved confirming that it is a highly conserved portion of the viral genome and suggesting that each conserved nucleotide may be functionally significant. Repeated occurrences of even non-constant nucleotides belong to a restricted number of genotypes raising the possibility that adaptation to new mosquito hosts and animal reservoirs such as those that accompanied spread of yellow fever virus from Africa to the Western Hemisphere are relevant. Knowledge of prolonged strings of invariable nucleotides in the 5’UTR has been used in designing a method for detecting YFV and may also be relevant for designing sequences for viral control of a variety of viruses.

show abstract

“…RNA interference (RNAi) is an important antiviral defense response in plants and invertebrates [176]. In DENV infection, knockdown of these components resulted in increased DENV replication in mammalian cells, suggesting that the RNAi pathways may play important roles in the cellular anti-DENV responses [177,178]. Additional evidence showed that DENV can interfere with RNAi pathways in human hepatocytes cells via NS4B and subgenomic flavivirus RNA (sfRNA) interactions with Dicer's ability to process small Please use Adobe Acrobat Reader to read this book chapter for free.…”

Section: Denv Infection and The Host Innate Immune Responsesmentioning

confidence: 99%

Dengue Immunopathogenesis: A Crosstalk between Host and Viral Factors Leading to Disease: Part I - Dengue Virus Tropism, Host Innate Immune Responses, and Subversion of Antiviral Responses

Puerta-Guardo¹,

Biering²,

Harris³

et al. 2020

Dengue Fever in a One Health Perspective

View full text Add to dashboard Cite

Dengue is the most prevalent emerging mosquito-borne viral disease, affecting more than 40% of the human population worldwide. Many symptomatic dengue virus (DENV) infections result in a relatively benign disease course known as dengue fever (DF). However, a small proportion of patients develop severe clinical manifestations, englobed in two main categories known as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Secondary infection with any of the four dengue virus serotypes (DENV1, -2, -3, and -4) is a risk factor to develop severe forms of dengue disease. DSS is primarily characterized by sudden and abrupt endothelial dysfunction, resulting in vascular leak and organ impairment, which may progress to hypovolemic shock and death. Severe DENV disease (DHF/DSS) is thought to follow a complex relationship between distinct immunopathogenic processes involving host and viral factors, such as the serotype cross-reactive antibody-dependent enhancement (ADE), the activation of T cells and complement pathways, the phenomenon of the cytokine storm, and the newly described viral toxin activity of the nonstructural protein 1 (NS1), which together play critical roles in inducing vascular leak and virus pathogenesis. In this chapter that is divided in two parts, we will outline the recent advances in our understanding of DENV pathogenesis, highlighting key viral-host interactions and discussing how these interactions may contribute to DENV immunopathology and the development of vascular leak, a hallmark of severe dengue. Part I will address the general features of the DENV complex, including the virus structure and genome, epidemiology, and clinical outcomes, followed by an updated review of the literature describing the host innate immune strategies as well as the viral mechanisms acting against and in favor of the DENV replication cycle and infection.

show abstract

Role of microRNAs in antiviral responses to dengue infection

Cited by 75 publications

References 110 publications

Circular RNA expression profiles significantly altered in UVA‑irradiated human dermal fibroblasts

Circular RNA expression profiles significantly altered in UVA‑irradiated human dermal fibroblasts

Constancy and Change in the 5’UTR of Yellow Fever Virus

Dengue Immunopathogenesis: A Crosstalk between Host and Viral Factors Leading to Disease: Part I - Dengue Virus Tropism, Host Innate Immune Responses, and Subversion of Antiviral Responses

Contact Info

Product

Resources

About