Role of microRNAs in osteogenesis of stem cells

Moghaddam, Tayebe; Neshati, Zeinab

doi:10.1002/jcb.28689

Cited by 30 publications

(18 citation statements)

References 206 publications

(399 reference statements)

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“…MSC osteogenic differentiation encompasses three phases: the first, also known as the proliferative phase, consists of the recruitment and expansion of osteoprogenitor cells; the second involves the cell maturation in pre-osteoblasts upon extracellular matrix (ECM) synthesis; and the third phase consists of the matrix mineralization [46].…”

Section: Msc Signaling Pathways In Osteogenic Differentiationmentioning

confidence: 99%

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

Mazziotta

Lanzillotti

Iaquinta

et al. 2021

IJMS

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Mesenchymal stem cells (MSCs) have been identified in many adult tissues and they have been closely studied in recent years, especially in view of their potential use for treating diseases and damaged tissues and organs. MSCs are capable of self-replication and differentiation into osteoblasts and are considered an important source of cells in tissue engineering for bone regeneration. Several epigenetic factors are believed to play a role in the osteogenic differentiation of MSCs, including microRNAs (miRNAs). MiRNAs are small, single-stranded, non-coding RNAs of approximately 22 nucleotides that are able to regulate cell proliferation, differentiation and apoptosis by binding the 3′ untranslated region (3′-UTR) of target mRNAs, which can be subsequently degraded or translationally silenced. MiRNAs control gene expression in osteogenic differentiation by regulating two crucial signaling cascades in osteogenesis: the transforming growth factor-beta (TGF-β)/bone morphogenic protein (BMP) and the Wingless/Int-1(Wnt)/β-catenin signaling pathways. This review provides an overview of the miRNAs involved in osteogenic differentiation and how these miRNAs could regulate the expression of target genes.

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Section: Msc Signaling Pathways In Osteogenic Differentiationmentioning

confidence: 99%

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

Mazziotta

Lanzillotti

Iaquinta

et al. 2021

IJMS

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“…48,53 Osx, an essential transcription factor for osteoblastic differentiation, played a crucial role in the differentiation and maturation of osteoblasts. 18 During the bone formation, BMP-2 or one of its co-signaling BMPs is imperative. 54 In this case, the expression levels of these key osteogenic regulation genes were established and are shown in Figure 8.…”

Section: Pcr Analysis and Western Blot Analysismentioning

confidence: 99%

“…15 The functions of several miRNAs in the bone regeneration process have already been investigated. [16][17][18] MiR-26a has been confirmed to regulate multiple pathways of osteogenic differentiation and promote bone restoration. 19,20 However, in the context of miRNA therapy, negatively charged miRNAs are ineffective in penetrating the cell membranes that are also negatively charged.…”

Section: Introductionmentioning

confidence: 99%

<p>Effects of miR-26a on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by a Mesoporous Silica Nanoparticle - PEI - Peptide System</p>

Yan¹,

Lü²,

Zhu³

et al. 2020

IJN

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Introduction: RNA-based therapy for bone repair and regeneration is a highly safe and effective approach, which has been extensively investigated in recent years. However, the molecular stability of RNA agents still remains insufficient for clinical application. High porosity, tunable size, and ideal biodegradability and biosafety are a few of the characters of mesoporous silicon nanoparticles (MSNs) that render them a promising biomaterial carrier for RNA treatment. Materials and Methods: In this study, a novel miR-26a delivery system was constructed based on MSNs. Next, we assessed the miRNA protection of the delivery vehicles. Then, rat bone marrow mesenchymal stem cells (rBMSCs) were incubated with the vectors, and the transfection efficiency, cellular uptake, and effects on cell viability and osteogenic differentiation were evaluated. Results: The results demonstrated that the vectors protected miR-26a from degradation in vitro and delivered it into the cytoplasm. A relatively low concentration of the delivery systems significantly increased osteogenic differentiation of rBMSCs. Conclusion: The vectors constructed in our study provide new methods and strategies for the delivery of microRNAs in bone tissue engineering.

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“…Receptor-phosphorylated/activated SMADs (R-SMADs) proteins includes SMAD2 and SMAD3 via TGF-β signaling and SMAD1, SMAD5 and SMAD8 via BMP pathway (Hata and Chen, 2016; Figure 4). R-SMADs from TGF-β/BMP pathways interact with SMAD4, that translocate into the nucleus complexed with SMAD2/3, thereby activating the expression of osteogenic genes, such as RUNX2 (Moghaddam and Neshati, 2019). On the other hand, TGF-β signaling pathway hampers the osteoblast differentiation by recruiting the histone deacetylases 4 and 5 (HDAC4/5) which inhibit the RUNX2 transcription (Kang et al, 2005;Thambyrajah et al, 2018).…”

Section: Figure 3 |mentioning

confidence: 99%

Long Non-coding RNAs and MicroRNAs Interplay in Osteogenic Differentiation of Mesenchymal Stem Cells

Lanzillotti

Mattei

Mazziotta

et al. 2021

Front. Cell Dev. Biol.

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Long non-coding RNAs (lncRNAs) have gained great attention as epigenetic regulators of gene expression in many tissues. Increasing evidence indicates that lncRNAs, together with microRNAs (miRNAs), play a pivotal role in osteogenesis. While miRNA action mechanism relies mainly on miRNA-mRNA interaction, resulting in suppressed expression, lncRNAs affect mRNA functionality through different activities, including interaction with miRNAs. Recent advances in RNA sequencing technology have improved knowledge into the molecular pathways regulated by the interaction of lncRNAs and miRNAs. This review reports on the recent knowledge of lncRNAs and miRNAs roles as key regulators of osteogenic differentiation. Specifically, we described herein the recent discoveries on lncRNA-miRNA crosstalk during the osteogenic differentiation of mesenchymal stem cells (MSCs) derived from bone marrow (BM), as well as from different other anatomical regions. The deep understanding of the connection between miRNAs and lncRNAs during the osteogenic differentiation will strongly improve knowledge into the molecular mechanisms of bone growth and development, ultimately leading to discover innovative diagnostic and therapeutic tools for osteogenic disorders and bone diseases.

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Role of microRNAs in osteogenesis of stem cells

Cited by 30 publications

References 206 publications

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

<p>Effects of miR-26a on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by a Mesoporous Silica Nanoparticle - PEI - Peptide System</p>

Long Non-coding RNAs and MicroRNAs Interplay in Osteogenic Differentiation of Mesenchymal Stem Cells

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