Role of Mmp-2 and Its Inhibitor Timp-2 As Biomarkers for Susceptibility to Systemic Lupus Erythematosus

Vira, Hemant J; Pradhan, Vandana; Umare, Vinod; Chaudhary, Ajay Kumar; Rajadhyksha, A; Nadkar, Milind; Ghosh, Kanjaksha; Nadkarni, Anita

doi:10.2217/bmm-2020-0180

Cited by 6 publications

(6 citation statements)

References 20 publications

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“…Furthermore, more evidence showed that TIMP-2 and MMP-2 expressions could play an important role in the development of other diseases. MMP-2, TIMP-2, and MMP-2/TIMP-2 ratios may act as biomarkers for susceptibility to systemic lupus erythematosus (SLE) [41]. Pathogenesis of chronic rhinosinusitis might also be related to the regulation of MMP-2 and TIMP-2 expressions.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dendrobium Polysaccharides on the Functions of Human Skin Fibroblasts and Expression of Matrix Metalloproteinase-2 under High-Glucose Conditions

Cao

et al. 2021

International Journal of Endocrinology

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The effects of Dendrobium polysaccharides (PDC) on the functions of human skin fibroblasts (HSFs) and expression of matrix metalloproteinase-2 under high-glucose conditions and exploration of the underlying mechanism remain unclear. We used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis and flow cytometry to evaluate the cell viability and apoptosis. The collagen levels were determined by the Sircol™ Collagen Assay. Real-time quantitative polymerase chain reaction (RT-PCR) was used to detect the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase inhibitor (TIMP-2) mRNA. We found the following: (1) under the high-glucose condition, the HSF cell viability, the expression of TIMP-2 mRNA, and the collagen levels were reduced, while the apoptosis rate and the expression of MMP-2 mRNA increased ( P < 0.05 ). (2) In the high-glucose + PDC group, the PDC reversed the changes in the collagen level, viability, and apoptosis rate of the HSF cells caused by high glucose, with the expression of protein and TIMP-2 mRNA increased and the level of MMP-2 mRNA decreased ( P < 0.05 ). This is the first time attempting to reveal that PDC can exhibit protective effects on HSF under high-glucose conditions, which may be related to the upregulation of the TIMP-2 expression and inhibition of the MMP-2 expression.

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Section: Discussionmentioning

confidence: 99%

Effects of Dendrobium Polysaccharides on the Functions of Human Skin Fibroblasts and Expression of Matrix Metalloproteinase-2 under High-Glucose Conditions

Cao

et al. 2021

International Journal of Endocrinology

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“…The most commonly found polymorphism is TNFα rs1800629, which is also associated with SLE, 128 IBD, 129 PD, 130 AR, 131 and IBS 132 . Other variants were found in more than one disease, namely, the IL1β rs16944 with UC 133 and AR, 131 the IL1RN rs2234663 with UC 129 and PD, 134 the IL6 rs1800795 with IBD 135 and IBS, 132 the TLR4 rs4986790 with IBD 136 and PD, 137 and finally, the TIMP2 rs8179090 with SLE 138 and PD 139 …”

Section: Autoimmune and Inflammatory Diseases Related To Ptbmentioning

confidence: 99%

Inflammatory factors, genetic variants, and predisposition for preterm birth

et al. 2021

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Preterm birth is a major clinical and public health challenge, with a prevalence of 11% worldwide. It is the leading cause of death in children younger than 5 years old and represents 70% of neonatal deaths and 75% of neonatal morbidity. Despite the clinical and public health significance, this condition's etiology is still unclear, and most of the cases are spontaneous. There are several known preterm birth risk factors, including inflammatory diseases and the genetic background, although the underlying molecular mechanisms are far from understood. The present review highlights the research advances on the association between inflammatory‐related genes and the increased risk for preterm delivery. The most associated genetic variants are the TNFα rs1800629, the IL1α rs17561, and the IL1RN rs2234663. Moreover, many of the genes discussed in this review are also implicated in pathologies involving inflammatory or autoimmune systems, such as periodontal disease, bowel inflammatory disease, and autoimmune rheumatic diseases. This review presents evidence suggesting a common genetic background to preterm birth, autoimmune and inflammatory diseases susceptibility.

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“…In addition, cellular overexpression and elevated serum levels of these four endopeptidases were observed in a wide variety of pathologies in which CD95/CD95L signalling has also been shown to contribute. For instance, MMP-2/MMP-3/MMP-7 serum levels are significantly increased in SLE and associated with disease activity, while elevated serum levels of MMP-12 have been observed in other systemic autoimmune diseases such as rheumatoid arthritis and systemic scleroderma [59][60][61][62]. MMP-2, -3, -7 and -12 have been reported to be overexpressed and associated with tumour propagation and poor cancer prognosis [59][60][61][62][63][64][65][66][67][68][69][70][71].…”

Section: Discussionmentioning

confidence: 99%

Metalloprotease‐mediated cleavage of CD95 ligand

et al. 2023

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CD95 is a member of the TNF receptor superfamily that is ubiquitously expressed in healthy and pathological tissues. Stimulation of CD95 by its physiological ligand CD95L induces its oligomerization leading in turn to the transduction of either apoptotic or nonapoptotic signals. CD95L can exist as both membrane‐anchored and soluble forms (sCD95L), the latter resulting from the proteolytic cleavage of the former. Candidate proteases able to achieve CD95L cleavage were identified as matrix metalloproteases (MMP) due to their demonstrated ability to cleave other TNF superfamily ligands. The main goal of this study was to systematically identify the MMP family members capable of cleaving CD95L and subsequently determine the corresponding cleavage sites. By using different orthogonal biochemical approaches and combining them with molecular modelling, we confirmed data from the literature regarding CD95L cleavage by MMP‐3 and MMP‐7. Moreover, we found that MMP‐2 and MMP‐12 can cleave CD95L and characterized their resulting cleavage sites. This study provides a systematic approach to analyse the cleavage of CD95L, which until now had only been poorly described.

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Role of Mmp-2 and Its Inhibitor Timp-2 As Biomarkers for Susceptibility to Systemic Lupus Erythematosus

Cited by 6 publications

References 20 publications

Effects of Dendrobium Polysaccharides on the Functions of Human Skin Fibroblasts and Expression of Matrix Metalloproteinase-2 under High-Glucose Conditions

Effects of Dendrobium Polysaccharides on the Functions of Human Skin Fibroblasts and Expression of Matrix Metalloproteinase-2 under High-Glucose Conditions

Inflammatory factors, genetic variants, and predisposition for preterm birth

Metalloprotease‐mediated cleavage of CD95 ligand

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