Role of MOG-stimulated Th1 type ‘light up’ (GFP+) CD4+T Cells for the Development of Experimental Autoimmune Encephalomyelitis (EAE)

Yura, Mamoru; Takahashi, Ichiro; Serada, Masashi; Koshio, Takehiro; Nakagami, Keiji; Yuki, Yoshikazu; Kiyono, Hiroshi

doi:10.1006/jaut.2001.0520

Cited by 48 publications

(31 citation statements)

References 33 publications

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“…This effect may block IFN-γ-mediated Th17 immune responses in EAE mice and inhibit EAE development. Moreover, IFN-γ is produced by Th1 cells, which also play an important role in EAE induction [60]. Our ELISA data demonstrated that LPS-treated DCs down-regulate the amount of IFN-γ, suggesting that LPStreated DCs may also affect the activity of Th1 cells via down-regulation of IFN-γ production.…”

Section: B220mentioning

confidence: 60%

Immunotherapy using lipopolysaccharide-stimulated bone marrow-derived dendritic cells to treat experimental autoimmune encephalomyelitis

Zhou

Ćirić

Zhang

et al. 2014

Clinical and Experimental Immunology

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SummaryLipopolysaccharide (LPS) produced by Gram-negative bacteria induces tolerance and suppresses inflammatory responses in vivo; however, the mechanisms are poorly understood. In this study we show that LPS induces apoptosis of bone marrow-derived dendritic cells (DCs) and modulates phenotypes of DCs. LPS treatment up-regulates expression of toleranceassociated molecules such as CD205 and galectin-1, but down-regulates expression of Gr-1 and B220 encephalomyelitis (EAE) development and down-regulates expression of retinoic acid-related orphan receptor gamma t (ROR-γt), interleukin (IL)-17A, IL-17F, IL-21, IL-22 and interferon (IFN)-γ in myelin oligodendrocyte glycoprotein (MOG)-primed CD4 + T cells in the peripheral environment. These results suggest that LPS-induced apoptotic DCs may lead to generation of tolerogenic DCs and suppress the activity of MOGstimulated effector CD4+ T cells, thus inhibiting the development of EAE in vivo. Our results imply a potential mechanism of LPS-induced tolerance mediated by DCs and the possible use of LPS-induced apoptotic DCs to treat autoimmune diseases such as multiple sclerosis.

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Section: B220mentioning

confidence: 60%

Immunotherapy using lipopolysaccharide-stimulated bone marrow-derived dendritic cells to treat experimental autoimmune encephalomyelitis

Zhou

Ćirić

Zhang

et al. 2014

Clinical and Experimental Immunology

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“…The first cell type we examined was the CD4 ϩ T cell, which is responsible for mediating EAE. 21,22 Since previous work had shown that TIMP-1 can be expressed by CD4 ϩ T cells, 23 we considered that the absence of TIMP-1 may have altered the development or magnitude of a myelin-specific CD4 ϩ T-cell response in MOG 35-55 -immunized mice. To test this, we isolated and pooled the cellular infiltrates from spinal cords of WT and TIMP-1 Ϫ/Ϫ mice, either acute phase (18 days) or chronic phase (52 days), after immunization with MOG and used flow cytometry to identify and quantify the antigen-specific T-cell response, using peptide-stimulated IFN-␥ production as a marker of activation.…”

Section: Cd4 ϩ T-cell Responses During Eaementioning

confidence: 99%

Persistent Macrophage/Microglial Activation and Myelin Disruption after Experimental Autoimmune Encephalomyelitis in Tissue Inhibitor of Metalloproteinase-1-Deficient Mice

Crocker

Whitmire

Frausto

et al. 2006

The American Journal of Pathology

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Increased leukocyte trafficking into the parenchyma during inflammatory responses in the central nervous system (CNS) isMultiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS). The etiology of MS is not known but inflammation and autoimmunity are central components of this disease. Many of the key features of MS can be modeled in rodents or nonhuman primates by immunization with myelin or myelin peptides that produce a condition called experimental autoimmune encephalomyelitis (EAE). In EAE, the development of myelin-specific T-cell responses and the infiltration of activated lymphocytes into the CNS parenchyma result in inflammation-mediated myelin injury. The infiltration of activated leukocytes into the CNS is required for immune-mediated myelin injury in both MS and EAE.1 Extravasation of immune cells into the CNS is facilitated by matrix metalloproteinases (MMPs). This large and diverse family of extracellular proteases are important regulators of tissue homeostasis and also are associated with cellular injury and pathology in a wide variety of CNS diseases, including MS. [2][3][4] In EAE, increased expression and activity of MMPs has been reported. 5 MMPs are produced predominantly by activated immune cells, 6,7 and MMPs facilitate the transmigration of these cells across the blood brain barrier by the proteolytic cleavage of substrates within the extracellular matrix.8 Deletion of MMP genes and pharmacological inhibition of MMP proteins lead to reduced immune cell trafficking into the CNS and attenuated demyelination during EAE.2,9 These findings indicate that elevated MMP expression and activity reported in the cerebrospinal fluid of MS patients likely reflects inadequate endogenous regulation

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“…Among CD4 + T cells, the T helper 1 cell subpopulation has been distinctively attributed a major role in the promotion of EAE (Yura et al 2001). Pathogenic properties of Th1 are mediated by highly pro-inflammatory and cytotoxic cytokines, such as interferongamma (IFN-g) (Bradley et al 1996;Xiao et al 2008) and tumor necrosis factor-alpha (TNF-a) (Selmaj and Raine 1988).…”

Section: Article In Pressmentioning

confidence: 99%

“…EAE is primarily mediated by CD4 + T cells specific for autoantigens in the CNS (Brostoff and Mason 1984;Yura et al 2001). During the induction phase of EAE, activated proinflammatory CD4 + T cells proliferate in the periphery and infiltrate the central nervous system (CNS) through interaction with endothelial cells, which facilitate the blood-brain barrier crossing (Cassan and Liblau 2007).…”

Section: Article In Pressmentioning

confidence: 99%

Cannabinoids and experimental models of multiple sclerosis

Kubajewska

Constantinescu

2010

Immunobiology

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Role of MOG-stimulated Th1 type ‘light up’ (GFP+) CD4+T Cells for the Development of Experimental Autoimmune Encephalomyelitis (EAE)

Cited by 48 publications

References 33 publications

Immunotherapy using lipopolysaccharide-stimulated bone marrow-derived dendritic cells to treat experimental autoimmune encephalomyelitis

Immunotherapy using lipopolysaccharide-stimulated bone marrow-derived dendritic cells to treat experimental autoimmune encephalomyelitis

Persistent Macrophage/Microglial Activation and Myelin Disruption after Experimental Autoimmune Encephalomyelitis in Tissue Inhibitor of Metalloproteinase-1-Deficient Mice

Cannabinoids and experimental models of multiple sclerosis

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