Role of N- and C-terminal domains and non-homologous region in co-refolding of Thermotoga maritima β-glucosidase

Kim, Bong-Jo; Mangala, Selanere L.; Muralidhara, B. K.; Hayashi, Kiyoshi

doi:10.1016/j.molcatb.2005.10.002

Journal of Molecular Catalysis B: Enzymatic

2005

DOI: 10.1016/j.molcatb.2005.10.002

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Role of N- and C-terminal domains and non-homologous region in co-refolding of Thermotoga maritima β-glucosidase

Bong-Jo Kim

Selanere L. Mangala²,

B. K. Muralidhara

et al.

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Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Epoxide hydrolase (EH) is such a nature-given tool for asymmetric hydrolysis of epoxides . In recent decades, much attention has been paid to microbial epoxide hydrolases.…”

mentioning

confidence: 99%

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Enantioconvergent Hydrolysis of Styrene Epoxides by Newly Discovered Epoxide Hydrolases in Mung Bean

Pan

et al. 2006

Org. Lett.

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[reaction: see text] Two novel epoxide hydrolases were discovered in mung bean (Phaseolus radiatus L.) for the first time, either of which can catalyze enantioconvergent hydrolysis of styrene epoxides. Their regioselectivity coefficients are more than 90% for the p-nitrostyrene oxide. Furthermore, the crude mung bean powder was also shown to be a cheap and practical biocatalyst, allowing a one-step asymmetric synthesis of chiral (R)-diols from racemic epoxides, in up to >99% ee and 68.7% overall yield (after recrystallization).

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“…Epoxide hydrolase (EH) is such a nature-given tool for asymmetric hydrolysis of epoxides . In recent decades, much attention has been paid to microbial epoxide hydrolases.…”

mentioning

confidence: 99%

“…In recent decades, much attention has been paid to microbial epoxide hydrolases. The two pioneer teams in this field have been the Faber and Furstoss groups . However, EHs from plant origins, discovered much earlier, have had scarce further practical application in asymmetric catalysis until 2004 in work by the Furstoss group …”

mentioning

confidence: 99%

Enantioconvergent Hydrolysis of Styrene Epoxides by Newly Discovered Epoxide Hydrolases in Mung Bean

Pan

et al. 2006

Org. Lett.

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Biotechnological production of enantiopure epoxides by enzymatic kinetic resolution

Choi

2009

Appl Microbiol Biotechnol

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Enantiopure epoxides are high value-added synthons for the production of pharmaceuticals, agrochemicals, as well as versatile fine chemicals and have broad scope of market demand for their applications. A major challenge in conventional organic synthesis is to generate such compounds in high enantiopurity with reasonable yield. Among possible chemical and biological technologies for enantiopure epoxide preparation, enzymatic kinetic resolution has been paid much attention with respect to its high enantioselectivity. Epoxide hydrolase (EH) has shown promising characteristics for the preparation of enantiopure epoxides and vicinal diols during enantioselective hydrolysis of racemic epoxides. EH is readily available from microbial resources thus it is being employed for biohydrolysis of a variety of epoxides. Recent technical progress in EH-catalyzed enantioselective hydrolysis is summarized in terms of exploration of novel EH, its functional improvement, high throughput assay, and preparative scale resolution process.

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Cloning, expression and enantioselective hydrolytic catalysis of a microsomal epoxide hydrolase from a marine fish, Mugil cephalus

Lee

Kim

et al. 2006

Biotechnol Lett

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The cDNA of a marine fish microsomal epoxide hydrolase (mEH) gene from Mugil cephalus was cloned by rapid amplification of cDNA ends (RACE) techniques. The homology model for the mEH of M. cephalus showed a characteristic structure of alpha/beta-hydrolase-fold main domain with a lid domain over the active site. The characteristic catalytic triad, consisting of Asp(238), His(444), and Glu(417), was highly conserved. The cloned mEH gene was expressed in Escherichia coli and the recombinant mEH exhibited (R)-preferred hydrolysis activity toward racemic styrene oxide. We obtained enantiopure (S)-styrene oxide with a high enantiopurity of more than 99% enantiomeric excess and yield of 15.4% by batch kinetic resolution of 20 mM racemic styrene oxide.

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Role of N- and C-terminal domains and non-homologous region in co-refolding of Thermotoga maritima β-glucosidase

Cited by 6 publications

References 28 publications

Enantioconvergent Hydrolysis of Styrene Epoxides by Newly Discovered Epoxide Hydrolases in Mung Bean

Enantioconvergent Hydrolysis of Styrene Epoxides by Newly Discovered Epoxide Hydrolases in Mung Bean

Biotechnological production of enantiopure epoxides by enzymatic kinetic resolution

Cloning, expression and enantioselective hydrolytic catalysis of a microsomal epoxide hydrolase from a marine fish, Mugil cephalus

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