Role of ocular albinism type 1 (OA1) GPCR in Asian gypsy moth development and transcriptional expression of heat-shock protein genes

Sun, Lili; Wang, Zhiying; Wu, Hongqu; Liu, Peng; Zou, Chuanshan; Xue, Xuting; Cao, Chuanwang

doi:10.1016/j.pestbp.2015.07.004

Cited by 10 publications

(10 citation statements)

References 36 publications

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“…Another example, in the nematode C. elegans, a neural GPCR gtr-1 is critically required for the induction of HSPs upon exposure to heat (Maman et al, 2013). Most recently, we explored the function of an Asian gypsy moth GPCR LdOA1 in modulating the expression of HSPs under insecticide stresses (Sun et al, 2016). In the present study, we observed the decreased expression of all eight HSPs tested in L. dispar larvae once knocking down the Ldmthl1 (Fig.…”

Section: Discussionsupporting

confidence: 56%

Physiological functions of a methuselah-like G protein coupled receptor in Lymantria dispar Linnaeus

Cao

Sun

et al. 2019

Pesticide Biochemistry and Physiology

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Insect G protein coupled receptors (GPCRs) have been identified as a highly attractive target for new generation insecticides discovery due to their critical physiological functions. However, few insect GPCRs have been functionally characterized. Here, we cloned the full length of a methuselah-like GPCR gene (Ldmthl1) from the Asian gypsy moth, Lymantria dispar. We then characterized the secondary and tertiary structures of Ldmthl1. We also predicted the global structure of this insect GPCR protein which is composed of three major domains. RNA interference of Ldmthl1 resulted in a reduction of gypsy moths' resistance to deltamethrin and suppressed expression of downstream stress-associated genes, such as P450s, glutathione S transferases, and heat shock proteins. The function of Ldmthl1 was further investigated using transgenic lines of Drosophila melanogaster. Drosophila with overexpression of Ldmthl1 showed significantly longer lifespan than control flies. Taken together, our studies revealed that the physiological functions of Ldmthl1 in L. dispar are associated with longevity and resistance to insecticide stresses. Potentially, Ldmthl1 can be used as a target for new insecticide discovery in order to manage this notorious forest pest.

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Section: Discussionsupporting

confidence: 56%

Physiological functions of a methuselah-like G protein coupled receptor in Lymantria dispar Linnaeus

Cao

Sun

et al. 2019

Pesticide Biochemistry and Physiology

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“…Recently, Cao et al (2014) reported that the expression of LdOA1 in 3 rd instar L. dispar larvae was significantly inhibited by deltamethrin, carbaryl and omethoate stresses during the 72 h period of exposure. Sun et al (2016) had provided additional confirmation that knockdown of the LdOA1 gene not only increased the mortality of 3 rd instar L. dispar larvae but also decreased their body weight, relative consumption rate (RCR), and approximate digestibility (AD) based on the nutrition utilization index. In the present study, we investigated the roles of LdOA1 in the regulation of deltamethrin tolerance and cytochrome P450 expression in both L. dispar and D. melanogaster .…”

Section: Discussionmentioning

confidence: 96%

“…To study the effects of deltamethrin on cytochrome P450 genes in L. dispar larvae microinjected with dsRNAs of LdOA1 or GFP (ds LdOA1 or ds GFP ), LC 20 value after 24 h of deltamethrin (15 mg/L) exposure was used as the treatment concentration (Sun et al, 2016). 15 mg/L of deltamethrin was added to the artificial diet and fed to the 3 rd instar larvae at 120 h after microinjection with ds LdOA1 and ds GFP .…”

Section: Methodsmentioning

confidence: 99%

Ocular Albinism Type 1 Regulates Deltamethrin Tolerance in Lymantria dispar and Drosophila melanogaster

et al. 2019

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The ocular albinism type 1 (OA1), a pigment cell-specific integral membrane glycoprotein, is a member of the G-protein-coupled receptor (GPCR) superfamily that binds to heterotrimeric G proteins in mammalian cells. We aimed to characterize the physiological functions an insect OA1 from Lymantria dispar ( LdOA1 ) employs in the regulation of insecticide tolerance. In the present study, we investigated the roles of LdOA1 in response to deltamethrin exposure in both L. dispar and Drosophila melanogaster . LdOA1 was expressed at the lowest level during the 4 th instar stage, while LdOA1 was significantly upregulated in the 5 th instar and male stages. Knockdown of LdOA1 by injecting dsRNA of LdOA1 into gypsy moth larvae caused a 4.80-fold higher mortality than in control larvae microinjected with dsRNA of GFP under deltamethrin stress. Nine out of 11 L. dispar CYP genes were significantly downregulated under deltamethrin stress in LdOA1 silenced larvae as compared to control larvae. Moreover, the LdOA1 gene was successfully overexpressed in D. melanogaster using transgenic technique. The deltamethrin contact assay showed that the LdOA1 overexpression in flies significantly enhanced the tolerance to deltamethrin compared to the control flies. Furthermore, the downstream Drosophila CYP genes were upregulated in the LdOA1 overexpression flies, suggesting LdOA1 may play a master switch role in P450-mediated metabolic detoxification. This study is the first report of an insect OA1 gene regulating insecticide tolerance and potentially playing a role in the regulation of downstream cytochrome P450 expression. These results contribute to the future development of novel insecticides targeting insect GPCRs.

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“…To reveal the role of CYP6EV11 in pollutant metabolism, RNAi technology was used in this study. RNAi has been successfully used in most insects like Lepidoptera and Coleoptera, but rarely used in Chironomidae [ 35 , 36 , 37 ]. Lu et al (2012) had successfully revealed two acetylcholinesterase genes ( TcAce1 and TcAce2 ) in Tribolium castaneum by the method of gene silencing [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 CYP6EV11 in Chironomus kiiensis Larvae Involved in Phenol Stress

Zhang

Chu

Sun

et al. 2018

IJMS

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Phenol is one of the organic pollutants which can cause water environment pollution. It is not only enriched in aquatic organisms but is also a serious threat to human health. Chironomus kiiensis is very sensitive to the contaminants in water and its cytochrome P450s are usually chosen as biomarkers for water pollution. To examine whether CYP6EV11 plays a role in the oxidative metabolism of phenol, we measured the silencing efficiency of CYP6EV11 and evaluated larval susceptibility to sublethal phenol levels by RNA interference (RNAi) technology. The results showed that the transcription of CYP6EV11 was found significantly up-regulated when the 4th instar C. kiiensis larvae were exposed to three doses of phenol. However, the transcriptional levels of CYP6EV11 were significantly suppressed by 92.7% in the 4th instar C. kiiensis larvae soaked in dsCYP6EV11 compared with those soaked in dsGFP for 6 h. The CYP6EV11 expression and mortality of the 4th instar C. kiiensis larvae with CYP6EV11 silencing were mostly decreased under phenol stress. Therefore, the CYP6EV11 gene may be used as a molecular biomarker for earlier warning and monitoring for water pollution.

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Role of ocular albinism type 1 (OA1) GPCR in Asian gypsy moth development and transcriptional expression of heat-shock protein genes

Cited by 10 publications

References 36 publications

Physiological functions of a methuselah-like G protein coupled receptor in Lymantria dispar Linnaeus

Physiological functions of a methuselah-like G protein coupled receptor in Lymantria dispar Linnaeus

Ocular Albinism Type 1 Regulates Deltamethrin Tolerance in Lymantria dispar and Drosophila melanogaster

Cytochrome P450 CYP6EV11 in Chironomus kiiensis Larvae Involved in Phenol Stress

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