Role of Oxidative DNA Damage and Antioxidative Enzymatic Defence Systems in Human Aging~!2008-08-08~!2008-10-29~!2008-12-05~!

Dittmar, Manuela; Knuth, Melanie; Beineke, Marc; Epe, Bernd

doi:10.2174/1874912700801010038

Cited by 14 publications

(9 citation statements)

References 47 publications

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“…Since ADMA competes with L-arginine for binding to nitric oxide synthase (Böger 2007), it may lead to electrons being diverted to molecular oxygen, with consequent increased production of superoxide rather than NO (Vásquez-Vivar et al 1998). The increased superoxide production may in turn call for an increase in SOD activity to facilitate the conversion of superoxide to hydrogen peroxide (Dittmar et al 2008). High SOD activity along with low GPx activity may lead to the accumulation of hydrogen peroxide and hydrogen peroxide-derived reactive oxygen species such as hydroxyl radicals.…”

Section: Discussionmentioning

confidence: 99%

The relationship of nitric oxide synthesis capacity, oxidative stress, and albumin-to-creatinine ratio in black and white men: the SABPA study

Mels

Huisman

Smith

et al. 2016

AGE

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Inadequate substrate availability and increased nitric oxide synthase inhibitor levels attenuate nitric oxide (NO) synthesis, whereas increased vascular oxidative stress may lead to inactivation of NO. We compared markers of NO synthesis capacity and oxidative stress in a bi-ethnic male population. Inter-relationships of ambulatory blood pressure and urinary albumin-to-creatinine ratio with NO synthesis capacity and oxidative stress markers were investigated. NO synthesis capacity markers (L-arginine, asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA)) and oxidative stress markers (serum peroxides, total glutathione, glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), and catalase) were measured. Black men displayed higher blood pressure and albumin-to-creatinine ratio (all p < 0.001), while NO synthesis capacity was more favorable (higher L-arginine and lower ADMA (p ≤ 0.003)). Antioxidant enzyme activities were similar except for the redox status markers (GR activity and GR/GPx ratio), which were upregulated in black men (p < 0.001). In black men, ADMA was inversely related to GPx activity (R (2) = 0.15; β = -0.20; p = 0.050) and GPx/SOD ratio (R (2) = 0.24; β = -0.37; p < 0.001), but none of these markers related to blood pressure or albumin-to-creatinine ratio. In white men, albumin-to-creatinine ratio was positively associated with ADMA (R (2) = 0.18; β = 0.39; p < 0.001) while ADMA was inversely related to GR activity (R (2) = 0.26; β = -0.29; p = 0.002) and GR/GPx ratio (R (2) = 0.25; β = -0.28; p = 0.003). Black men with elevated blood pressure and albumin-to-creatinine ratio displayed a favorable NO synthesis capacity. This may be counteracted by increased inactivation of NO, although it was not linked to vascular or renal phenotypes. In white men, reduced NO synthesis capacity may lower NO bio-availability, thereby influencing the albumin-to-creatinine ratio.

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Section: Discussionmentioning

confidence: 99%

The relationship of nitric oxide synthesis capacity, oxidative stress, and albumin-to-creatinine ratio in black and white men: the SABPA study

Mels

Huisman

Smith

et al. 2016

AGE

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“…This result can be due to the higher catalase activity and SOD/GPx activity ratio. Previous reports showed that SOD/GPx activity ratio is considered a better enzymatic antioxidant indicator than the absolute enzyme activity (de Haan et al, 1995;Dittmar et al, 2008), which can contribute to protect the nicotine group against lipid peroxidation. Moreover, the N offspring showed an increase of plasma albumin and total bilirubin, reinforcing the protection system against the lipid peroxidation.…”

Section: Tablementioning

confidence: 99%

Maternal nicotine exposure leads to higher liver oxidative stress and steatosis in adult rat offspring

Conceição

Peixoto-Silva

Pinheiro

et al. 2015

Food and Chemical Toxicology

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“…The level of SOD was increased in HepG2 cells only at concentration 0.1 mM while in V79 cells it was not increased at all. Dittmar et al [3], who investigated the role of oxidative DNA damage in human aging, revealed that in blood of older people (that contains a higher amount of oxidative DNA lesions) the SOD/GPx ratio is lowered. This means that the activity of GPx is elevated and the amount of DNA lesions is inversely related to SOD activity.…”

Section: Discussionmentioning

confidence: 99%

“…Oxidative stress occurs in a cell or in a tissue if the concentration of the reactive oxygen species (ROS) and free radicals exceeds their antioxidant capability [1]. As the highly reactive forms of oxygen from both exogenous and endogenous sources may be involved in the pathogenesis of many diseases [2] as well as in the process of aging [3], the acquisition of new knowledge about antioxidant defense mechanisms of cells is very important. Research of molecular bases of antioxidant responses may benefit from the use of established cultures of mammalian cells in vitro.…”

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confidence: 99%

Influence of different chemical agents (H2O2, t-BHP and MMS) on the activity of antioxidant enzymes in human HepG2 and hamster V79 cells; relationship to cytotoxicity and genotoxicity

Slameñová¹,

Kozics²,

Melušová³

et al. 2015

neo

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We investigated activities of antioxidant enzymes (AEs), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in human HepG2 and hamster V79 cells treated with a scale of concentrations of hydrogen peroxide (H2O2), tert-butyl hydroperoxide (t-BHP) and methyl methanesulfonate (MMS). Cytotoxicity and genotoxicity of these substances were evaluated simultaneously. We have found out that H2O2, t-BHP and MMS predictably induce significant concentration-dependent increase of DNA lesions in both cell lines. Cytotoxicity detected in V79 cells with help of PE test was in a good conformity with the level of DNA damage. MTT test has proved unsuitable, except for MMS-treated V79 cells. Compared with human cells HepG2, hamster cells V79 manifested approximately similar levels of SOD and CAT but ten times higher activity of GPx. Across all concentrations tested the most significant increase of activity of the enzyme CAT was found in H2O2- and t-BHP-treated HepG2 cells, of the enzyme SOD in t-BHP- and MMS-treated V79 cells, and of the enzyme GPx in H2O2-treated V79 cells. We suggest that stimulation of enzyme activity by the relevant chemical compounds may result from transcriptional or post-transcriptional regulation of the expression of the genes CAT, SOD and GPx. Several authors suggest that moderate levels of toxic reactants can induce increase of AEs activities, while very high levels of reactants can induce their decrease, as a consequence of damage of the molecular machinery required to induce AEs. Based on a great amount of experiments, which were done and described within this paper, we can say that the above mentioned principle does not apply in general. Only the reactions of t-BHP affected HepG2 cells were consistent with this idea.

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Role of Oxidative DNA Damage and Antioxidative Enzymatic Defence Systems in Human Aging~!2008-08-08~!2008-10-29~!2008-12-05~!

Cited by 14 publications

References 47 publications

The relationship of nitric oxide synthesis capacity, oxidative stress, and albumin-to-creatinine ratio in black and white men: the SABPA study

The relationship of nitric oxide synthesis capacity, oxidative stress, and albumin-to-creatinine ratio in black and white men: the SABPA study

Maternal nicotine exposure leads to higher liver oxidative stress and steatosis in adult rat offspring

Influence of different chemical agents (H2O2, t-BHP and MMS) on the activity of antioxidant enzymes in human HepG2 and hamster V79 cells; relationship to cytotoxicity and genotoxicity

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