Role of Oxidative Stress in C. jejuni Inactivation During Freeze-Thaw Treatment

Garénaux, Amélie; Ritz, M.; Jugiau, F.; Rama, F.; Fédérighi, Michel; Jonge, R. de

doi:10.1007/s00284-008-9289-3

Cited by 28 publications

(21 citation statements)

References 12 publications

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“…In contrast, at higher temperatures (at 42 or 52°C), no viable C. coli cells were detected after 24 h using either bacterial culture or RT-qPCR method. These findings are in agreement with data from previous study reported by Garénaux et al (2009) who revealed that a cross protection between the cold shock response and oxidative stress response might explain the increased resistance of bacteria at low temperature. In addition, it has been shown that superoxide dismutase, as well as other oxidized stress related proteins were over-expressed at 4°C (Stintzi, 2003).…”

Section: Discussionsupporting

confidence: 94%

Fate and Survival of Campylobacter coli in Swine Manure at Various Temperatures

Bui¹,

Wolff²,

Madsen³

et al. 2011

Front. Microbio.

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Campylobacter coli is the most common Campylobacter species found in pig (95%), but the ability of this bacterium to survive in swine manure as well as the potential for causing human illness are poorly understood. We present here laboratory-scale experiments to investigate the effect of temperature on the survival of C. coli in spiked swine manure samples at temperatures from 4 to 52°C. The survival of C. coli during storage for 30 days was studied by three different methods: bacterial culture (plate counting), DNA qPCR, and mRNA RT-qPCR. The results indicate that C. coli could survive in swine manure up to 24 days at 4°C. At higher temperatures, this bacterium survived only 7 days (15°C) or 6 days (22°C) of storage. The survival of C. coli was extremely short (few hours) in samples incubated at 42 and 52°C. The results from the RT-qPCR method were consistent with the data from the bacterial culture method, indicating that it detected only viable C. coli cells, thus eliminating false-positive resulting from DNA from dead C. coli cells.

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Section: Discussionsupporting

confidence: 94%

Fate and Survival of Campylobacter coli in Swine Manure at Various Temperatures

Bui¹,

Wolff²,

Madsen³

et al. 2011

Front. Microbio.

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“…For instance, in Campylobacter superoxide dismutase (SOD) was found to play an important role in cryotolerance (18,35), as well as in the survival in macrophages (29) and in the colonization of chickens (31). In L. monocytogenes, SOD is required for virulence in the murine model and is controlled posttranslationally by phosphorylation (1).…”

Section: Discussionmentioning

confidence: 99%

Role of Growth Temperature in Freeze-Thaw Tolerance of Listeria spp

Azizoglu

Osborne

Wilson

et al. 2009

Appl Environ Microbiol

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The food-borne pathogen Listeria monocytogenes can grow in a wide range of temperatures, and several key virulence determinants of the organism are expressed at 37°C but are strongly repressed below 30°C. However, the impact of growth temperature on the ability of the bacteria to tolerate environmental stresses remains poorly understood. In other microorganisms, cold acclimation resulted in enhanced tolerance against freezing and thawing (cryotolerance). In this study, we investigated the impact of growth temperature (4, 25, and 37°C) on the cryotolerance of 14 strains of L. monocytogenes from outbreaks and from food processing plant environments and four strains of nonpathogenic Listeria spp. (L. welshimeri and L. innocua). After growth at different temperatures, cells were frozen at ؊20°C, and repeated freeze-thaw cycles were applied every 24 h. Pronounced cryotolerance was exhibited by cells grown at 37°C, with a <1-log decrease after 18 cycles of freezing and thawing. In contrast, freeze-thaw tolerance was significantly reduced (P < 0.05) when bacteria were grown at either 4 or 25°C, with log decreases after 18 freeze-thaw cycles ranging from 2 to >4, depending on the strain. These findings suggest that growth at 37°C, a temperature required for expression of virulence determinants of L. monocytogenes, is also required for protection against freeze-thaw stress. The negative impact of growth at low temperature on freeze-thaw stress was unexpected and has not been reported before with this or other psychrotrophic microorganisms.

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“…Campylobacter is probably also inactivated by an oxidative burst when high pressure treatment is applied [16]. Moreover, oxidative stress and redox-related proteins were found to be over-expressed in C. jejuni stressed with paraquat, a strong oxidizing agent [17].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Adhesion of Campylobacter jejuni to Abiotic Surfaces Is Mediated by Membrane Proteins in Oxygen-Enriched Conditions

et al. 2012

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Campylobacter jejuni is responsible for the major foodborne bacterial enteritis in humans. In contradiction with its fastidious growth requirements, this microaerobic pathogen can survive in aerobic food environments, suggesting that it must employ a variety of protection mechanisms to resist oxidative stress. For the first time, C. jejuni 81–176 inner and outer membrane subproteomes were analyzed separately using two-dimensional protein electrophoresis (2-DE) of oxygen-acclimated cells and microaerobically grown cells. LC-MS/MS analyses successfully identified 42 and 25 spots which exhibited a significantly altered abundance in the IMP-enriched fraction and in the OMP-enriched fraction, respectively, in response to oxidative conditions. These spots corresponded to 38 membrane proteins that could be grouped into different functional classes: (i) transporters, (ii) chaperones, (iii) fatty acid metabolism, (iv) adhesion/virulence and (v) other metabolisms. Some of these proteins were up-regulated at the transcriptional level in oxygen-acclimated cells as confirmed by qRT-PCR. Downstream analyses revealed that adhesion of C. jejuni to inert surfaces and swarming motility were enhanced in oxygen-acclimated cells or paraquat-stressed cells, which could be explained by the higher abundance of membrane proteins involved in adhesion and biofilm formation. The virulence factor CadF, over-expressed in the outer membrane of oxygen-acclimated cells, contributes to the complex process of C. jejuni adhesion to inert surfaces as revealed by a reduction in the capability of C. jejuni 81–176 ΔCadF cells compared to the isogenic strain.Taken together, these data demonstrate that oxygen-enriched conditions promote the over-expression of membrane proteins involved in both the biofilm initiation and virulence of C. jejuni.

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Role of Oxidative Stress in C. jejuni Inactivation During Freeze-Thaw Treatment

Cited by 28 publications

References 12 publications

Fate and Survival of Campylobacter coli in Swine Manure at Various Temperatures

Fate and Survival of Campylobacter coli in Swine Manure at Various Temperatures

Role of Growth Temperature in Freeze-Thaw Tolerance of Listeria spp

Enhanced Adhesion of Campylobacter jejuni to Abiotic Surfaces Is Mediated by Membrane Proteins in Oxygen-Enriched Conditions

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