Role of Paris PM2.5 components in the pro-inflammatory response induced in airway epithelial cells

Baulig, Augustin; Singh, Seema; Marchand, Alexandre; Schins, Roel P. F.; Barouki, Robert; Garlatti, Michèle; Marano, Francelyne; Baeza‐Squiban, Armelle

doi:10.1016/j.tox.2009.05.007

Cited by 60 publications

(49 citation statements)

References 37 publications

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“…It is possible that exposure to PM disrupts the integrity of pulmonary epithelial cells and thereby releases inflammatory cytokines that activate DC and drive Th2-mediated allergic immunity as suggested by others [57,58] . An attractive mechanism responsible for DC activation by epithelial cells suggests release of granulocyte-macrophage colony-stimulating factor and thymic stromal lymphopoietin (TSLP) on exposure to particulate pollutants that may also involve oxidative stress and the generation of reactive oxygen species in the activation of pulmonary DC [11,42,54,57,59] .…”

Section: Discussionmentioning

confidence: 99%

“…Environmental PM is a complex mixture of particles with varying properties. How the properties of exposure to PM contribute to altered early pulmonary immune responses and dendritic cell (DC) activation in vivo are unclear but may be attributable to cellular contact-dependent particle size and surface area of the PM species, elemental and hydrocarbon constitution and an inherent ability to differentially promote oxidative stress and acute inflammation [10][11][12][13][14] .…”

mentioning

confidence: 99%

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Activation of Pulmonary Dendritic Cells and Th2-Type Inflammatory Responses on Instillation of Engineered, Environmental Diesel Emission Source or Ambient Air Pollutant Particles in vivo

et al. 2010

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The biological effects of acute particulate air pollution exposure in host innate immunity remain obscure and have relied largely on in vitro models. We hypothesized that single acute exposure to ambient or engineered particulate matter (PM) in the absence of other secondary stimuli would activate lung dendritic cells (DC) in vivo and provide information on the early immunological events of PM exposure and DC activation in a mouse model naïve to prior PM exposure. Activation of purified lung DC was studied following oropharyngeal instillation of ambient particulate matter (APM). We compared the effects of APM exposure with that of diesel-enriched PM (DEP), carbon black particles (CBP) and silver nanoparticles (AgP). We found that PM species induced variable cellular infiltration in the lungs and only APM exposure induced eosinophilic infiltration. Both APM and DEP activated pulmonary DC and promoted a Th2-type cytokine response from naïve CD4+ T cells ex vivo. Cultures of primary peribronchial lymph node cells from mice exposed to APM and DEP also displayed a Th2-type immune response ex vivo. We conclude that exposure of the lower airway to various PM species induces differential immunological responses and immunomodulation of DC subsets. Environmental APM and DEP activated DC in vivo and provoked a Th2 response ex vivo. By contrast, CBP and AgP induced altered lung tissue barrier integrity but failed to stimulate CD4+ T cells as effectively. Our work suggests that respirable pollutants activate the innate immune response with enhanced DC activation, pulmonary inflammation and Th2-immune responsiveness.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Activation of Pulmonary Dendritic Cells and Th2-Type Inflammatory Responses on Instillation of Engineered, Environmental Diesel Emission Source or Ambient Air Pollutant Particles in vivo

et al. 2010

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“…The PM-induced human health effects are still not completely understood, but oxidative stress and inflammatory reaction seem to be of fundamental importance (Baulig et al 2009. Lodovici and Bigagli 2011.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic response in human lung epithelial cells and ion characteristics of urban-air particles from Torino, a northern Italian city

Alessandria¹,

Schilirò²,

Degan³

et al. 2014

Environ Sci Pollut Res

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“…Epidemiological and toxicological studies have shown associations between adverse health effects and airborne particulate matter (Dockery et al, 1993;Monn and Becker 1999;Oberdörster et al, 2000;Schaumann et al, 2004;Baulig et al, 2009;Peters et al, 2009). Recent improvements in measurement techniques allowed the study and demonstration of correlations between ultrafine particles (UFP, particle diameter d < 100 nm) and adverse health effects (Peters et al, 1997a, b;Donaldson et al, 1998, Oberdörster et al, 2000, 2001Stölzel et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Size Resolved Particle Number Emission Factors of Motorway Traffic Differentiated between Heavy and Light Duty Vehicles

Nickel

Kaminski

Hellack

et al. 2013

Aerosol Air Qual. Res.

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It is well accepted that airborne particles can induce adverse health effects dependent on the source, composition, morphology and size. Studies indicate that ultrafine particles (diameter < 100 nm) are of specific importance. Therefore, upwind and downwind field measurements of particle number size distributions (14-750 nm), nitrogen oxides, PM 10 and PM 1 mass concentrations were performed to derive information on sources of those types of particles from motorways. The measurement stations were located at a motorway in a rural area with flat terrain and unhindered air flow situation. The mean particle number concentration was 20,900 #/cm 3 downwind and 3,400 #/cm 3 upwind of the motorway. The highest total particle number concentration at the downwind station was 141,000 #/cm 3 . About 90% of these particles were < 100 nm. The measured data were used to derive size-dependent emission factors (EF) using the NO x tracer method. This method is based on listed NO x EF (HBEFA, 2010). The average total particle number EF per vehicle was determined to be 3.5 × 10 14 particles/km. The average particle EF was 2.1 × 10 14 particles/km and 11.8 × 10 14 particles/km for light duty vehicles (LDV) and heavy duty vehicles (HDV). The higher EF for HDV is mainly caused by particles with diameters below 50 nm. The comparison of EF from the literature show the importance of the particle size range investigated. Especially particles at the lower size detection limit contribute to total particle number concentrations and hence determine the EF significantly. In the EURO V directive, particle number emission limits of 6 × 10 11 particles/km were set for diesel passenger cars. This value is defined for non-volatile particles > 23 nm. The EF for the given size range (> 23 nm) determined in this study were significantly higher with 1.0 × 10 14 for LDV.

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Role of Paris PM2.5 components in the pro-inflammatory response induced in airway epithelial cells

Cited by 60 publications

References 37 publications

Activation of Pulmonary Dendritic Cells and Th2-Type Inflammatory Responses on Instillation of Engineered, Environmental Diesel Emission Source or Ambient Air Pollutant Particles in vivo

Activation of Pulmonary Dendritic Cells and Th2-Type Inflammatory Responses on Instillation of Engineered, Environmental Diesel Emission Source or Ambient Air Pollutant Particles in vivo

Cytotoxic response in human lung epithelial cells and ion characteristics of urban-air particles from Torino, a northern Italian city

Size Resolved Particle Number Emission Factors of Motorway Traffic Differentiated between Heavy and Light Duty Vehicles

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