Role of phage-antibiotic combination in reducing antibiotic resistance in Staphylococcus aureus

Jo, Ara; Kim, Jeongjin; Ding, Tian; Ahn, Juhee

doi:10.1007/s10068-016-0192-6

Cited by 33 publications

(19 citation statements)

References 22 publications

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“…This strategy exerts a heavy selective pressure on the targeted bacteria which induces a heavier fitness cost to them. This cost would drive resistant mutants eventually appearing towards being fitter to resist to the phage and antibiotic and less fit to reproduce and infect their hosts (Torres-Barceló et al, 2014;Jo et al, 2016).…”

Section: Giving a Second Chance To Antibiotics With Phage-antibioticmentioning

confidence: 99%

Overcoming the challenges of phage therapy for industrial aquaculture: A review

2019

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Aquaculture is the fastest-growing sector in food industry. Its development is powered by the intensification of the production which increased bacterial disease occurrence and spreading. As aquaculture deeply relies on a massive prophylactic and therapeutic use of antibiotics, it is threatened by the emergence of multi drug resistant bacteria. The stalled development of new antibiotics makes finding new therapeutic solutions a burning issue. Thanks to their specific host range, their ability to treat both the farmed species and the environment, their limited ecological impact and their abundance in the environment, bacteriophages represent a promising sustainable solution to control pathogenic aquaculture bacteria. In this review we discuss the interest of phage biocontrol for aquaculture and how can bacterial resistance, ecological, pharmacological and production related issues be solved.

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Section: Giving a Second Chance To Antibiotics With Phage-antibioticmentioning

confidence: 99%

Overcoming the challenges of phage therapy for industrial aquaculture: A review

2019

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“…For a Siphoviridae phage used in the study, the effect which can be defined as completely synergistic was reported to be observed only after a 300 min incubation ‐ (Knezevic, Curcin, Aleksic, Petrusic, & Vlaski, ). Synergistic antimicrobial efficacy of phages with antibiotics against S. aureus was evaluated in another study where the obtained results showed that the synergism between phage and antibiotic was more prominent in S. aureus KACC 13236 strains treated with SA11 phage in the presence of cefoxitin, chloramphenicol, and polymyxin (Jo, Kim, Ding, & Ahn, ). It was also stated that the cells were all damaged or killed as a result of applying the combined treatment.…”

Section: Discussionmentioning

confidence: 99%

“…In the last few years, studies involving the interaction of bacteriophages with various components have been frequently found in the literature. The majority of these studies are based on phage-antibiotic synergism and the elimination of biofilms (Chaudhry et al, 2017;Nouraldin et al, 2016) or bacteria such as Acinetobacter baumannii (Jansen et al, 2018), Money, 2017), Pseudomonas aeruginosa (Lin et al, 2018;Uchiyama et al, 2018), S. aureus (Jo, Kim, Ding, & Ahn, 2016). There are also studies examining the interaction of phages with different agents such as honey (Oliveira et al, 2017) or disinfectants (Agún et al, 2018).…”

Section: S Aureusmentioning

confidence: 99%

Investigation of different interactions between Staphylococcus aureus phages and pomegranate peel, grape seed, and black cumin extracts

Tayyarcan

Soykut

Yilmaz

et al. 2019

Journal of Food Safety

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Today, antibiotic resistance is one of the most crucial problems so that researchers have begun to search for alternatives to antibiotics. Among these alternatives, phage therapy and phytotherapy became prominent. The present study aims to put forward a different approach by combining these two methods. For this purpose, Staphylococcus aureus phages were isolated from commercial mixtures. After that, extraction of phenolic compounds from pomegranate peels (Ps), grape seeds (GSs), and black cumin (NS) was carried out and antimicrobial activities were determined. Phage‐phenolic interactions were investigated by examining the effect of phenolic extracts on phage plaque sizes and then on phage titer and bacteria count. As a result, it was found that only the NS extract had a positive effect on phage activity and increased the size of phage plaques. However, no synergistic effect was observed in experiments performed in liquid media. It was also found that P and GS extracts inhibited phage activity. Practical Applications The present study was focused on effects of extracts obtained from pomegranate peel, grape seed, and black cumin on Staphylococcus aureus phage activity. It is known that antibiotic resistance constitutes a major obstacle to the pathogen elimination. In this study, phage therapy and phytotherapy, which are prominent methods in pathogen elimination, have been combined and the effect of phenolic compounds isolated from natural sources on phage activity and bacteria count have been examined. Given the results of the current study, it has been demonstrated that the combined use of phages and antimicrobial compounds could be an important alternative to combating pathogens. It is thought that this combined method should be taken into consideration both in the practice of phage therapy on humans and animals, and in applications in the food industry.

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“…Disks of erythromycin (15 μg) and vancomycin (30 μg) were placed on the Mueller-Hinton agar plates containing S. aureus (105CFU/mL) alone and with phage (106 PFU/mL). After 18-h incubation at 37° C, the diameter of the clear inhibition zone was measured (Jo et al, 2016) 3. Results…”

Section: Disk Diffusion Susceptibility Testmentioning

confidence: 99%

Isolation and characterization of six gamma-irradiated bacteriophages specific for MRSA and VRSA isolated from skin infections

Mahmoud

Ahmed

Abo-Senna

et al. 2021

Journal of Radiation Research and Applied Sciences

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Although skin infections are usually uncomplicated, it may indicate systemic disease or lead to life-threatening systemic infections. Staphylococcus aureus is the most common cause of skin infections. Treatment is complicated by continuous emergence of resistant S. aureus especially methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant S. aureus (VRSA). Therefore, searching for new alternatives is essential to combat this escalating antibiotic resistance. Bacteriophage therapy is a promising solution in the post antibiotic area. Bacteriophage therapy confers safety and higher rate of success. In the present study, 10 bacteriophages (belonging to the family Siphoviridae and Myoviridae) were isolated with lytic activity against MRSA &VRSA isolated from skin infections. The strongest six of ten phages were characterized morphologically by a JOEL_JEM_1010 electron microscope, physically through evaluating their lytic activity against wide ranges of temperature (35-95° C), PH (3-10) and radiation doses (0.5-2.5kGy) and biologically by evaluating their host ranges and phage longevity (1-120 day) in vitro. Finally, the anti-Staphylococcal effect of the isolated bacteriophages was tested and revealed that they have alone activity (10, 12) and synergistic activity with vancomycin and erythromycin by increasing their inhibition zone diameters (16, 22) mm, respectively.

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Role of phage-antibiotic combination in reducing antibiotic resistance in Staphylococcus aureus

Cited by 33 publications

References 22 publications

Overcoming the challenges of phage therapy for industrial aquaculture: A review

Overcoming the challenges of phage therapy for industrial aquaculture: A review

Investigation of different interactions between Staphylococcus aureus phages and pomegranate peel, grape seed, and black cumin extracts

Isolation and characterization of six gamma-irradiated bacteriophages specific for MRSA and VRSA isolated from skin infections

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