Role of phosphate solubilizing Burkholderia spp. for successful colonization and growth promotion of Lycopodium cernuum L. (Lycopodiaceae) in lateritic belt of Birbhum district of West Bengal, India

Abstract

Profuse growth of Lycpodium cernuum L. was found in phosphate deficient red lateritic soil of West Bengal, India. Interaction of vesicular-arbuscular mycorrhiza (VAM) with Lycopodium rhizoids were described earlier but association of PGPR with their rhizoids were not studied. Three potent phosphate solubilizing bacterial strains (P4, P9 and P10) associated with L. cernuum rhizoids were isolated and identified by 16S rDNA homologies on Ez-Taxon database as Burkholderia tropica, Burkholderia unamae and Burkholderia cepacia respectively. Day wise kinetics of phosphate solubilization against Ca3(PO4)2 suggested P4 (580.56±13.38 μg ml(-1)) as maximum mineral phosphate solubilizer followed by P9 (517.12±17.15 μg ml(-1)) and P10 (485.18±14.23 μg ml(-1)) at 28 °C. Release of bound phosphates by isolated strains from ferric phosphate (FePO4), aluminum phosphate (AlPO4) and four different complex rock phosphates indicated their very good phosphate solubilizng efficacy. Nitrogen independent solubilizition also supports their nitrogen fixing capabilities. Inhibition of P solubilization by calcium salts and induction by EDTA suggested pH dependent chelation of metal cations by all of the isolates. Rhizoidal colonization potentials of Burkholderia spp. were confirmed by in planta experiment and also using scanning electron microscope (SEM). Increases of total phosphate content in Lycopodium plants upon soil treatment with these isolates were also recorded. In addition siderophore production on CAS agar medium, tryptophan dependent IAA production and antifungal activities against pathogenic fungi by rhizospheric isolates deep-rooted that they have definite role in nutrient mobilization for successful colonization of L. cernuum in nutrient deficient lateritic soil.

Improved iron acquisition ofAstragalus sinicusunder low iron-availability conditions by soil-borne bacteriaBurkholderia cepacia

“…A large number of studies have shown that application of PGPR enhances the tolerance of plants to multiple abiotic stresses such as nutrient deficiency, water deprivation and soil alkalization (Dey et al 2004;Sherameti et al 2008;Zhou et al 2016), indicating that beneficial soil bacteria can be employed to increase plant's absorption of nutrient elements. B. cepacia is one of the more important PGPR that have been widely used to improve plant growth, stress adaption and pathogen invasion (Singh et al 2013;Zhao et al 2014;Ghosh et al 2016). In this study, a novel role of B. cepacia JFW16 in regulating plant uptake of Fe was observed, which was positively associated with the activated plant's Fe acquisition systems.…”

“…However, alkaline soils become the major constraints to the distribution and biomass of crop in agriculture Jia et al 2015). Recently, soilborne bacteria Burkholderia cepacia has been attracted considerable attentions due to its diverse roles such as enhancement of nutrient use efficiency and control of fungal infection in host plants (Singh et al 2013;Zhao et al 2014;Ghosh et al 2016). Here, we examined the activity of B. cepacia (strain JFW16) in plant Fe assimilation.…”

Bioremediation of heavy metals using an endophytic bacterium Paenibacillus sp. RM isolated from the roots of Tridax procumbens

“…The results are in agreement with previous study reporting ACC deaminase activity of endophytic bacteria in Phytolacca Americana (Zhang et al 2015). Phosphate solubilization potential is very common among endophytic bacteria (Ghosh et al 2016). In this study, a clear zone around the isolates RM2, RM3, and RM colonies in Pikovskaya medium indicated the phosphate solubilization potential of the isolates.…”

Long-Term Rock Phosphate Fertilization Impacts the Microbial Communities of Maize Rhizosphere

“…(Burkholderiaceae) and Bacillus sp. (Bacillaceae) are also reported to be effective RP solubilizers, both in vitro assays (Gomes et al, 2014; Ghosh et al, 2016) or inoculated in plants (Baig et al, 2014; Stephen et al, 2015; Wahid et al, 2016). Furthermore, Burkholderia and Herbaspirillum are both diazotrophs.…”