Role of photobleaching process of indocyanine green for killing neuroblastoma cells

Clutter, Elwin D.; Chen, Liaohai L.; Wang, Rong R.

doi:10.1016/j.bbrc.2021.12.033

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…The next iterations could use the minor decrease in SNR with comparative CNR at 1 versus 10 ms (Figs. 2 and 3), with gated laser emission to reduce exposure of the agent at no cost to tumor delineation, thus reducing photobleaching (40)(41)(42)(43). The mean necropsy-based muscle-to-tumor ratio of 22.6 at 96 h provided binary and unambiguous tumor delineation, with tumor values that were statistically significant compared with liver values (Fig.…”

Section: Discussionmentioning

confidence: 99%

Ambient Light Resistant Shortwave Infrared Fluorescence Imaging for Preclinical Tumor Delineation via the pH Low-Insertion Peptide Conjugated to Indocyanine Green

Mc Larney,

Kim,

Roberts

et al. 2023

J Nucl Med

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Shortwave infrared (900-1,700 nm) fluorescence imaging (SWIRFI) has shown significant advantages over visible (400-650 nm) and nearinfrared (700-900 nm) fluorescence imaging (reduced autofluorescence, improved contrast, tissue resolution, and depth sensitivity). However, there is a major lag in the clinical translation of preclinical SWIRFI systems and targeted SWIRFI probes. Methods: We preclinically show that the pH low-insertion peptide conjugated to indocyanine green (pHLIP ICG), currently in clinical trials, is an excellent candidate for cancer-targeted SWIRFI. Results: pHLIP ICG SWIRFI achieved picomolar sensitivity (0.4 nM) with binary and unambiguous tumor screening and resection up to 96 h after injection in an orthotopic breast cancer mouse model. SWIRFI tumor screening and resection had ambient light resistance (possible without gating or filtering) with outstanding signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) values at exposures from 10 to 0.1 ms. These SNR and CNR values were also found for the extended emission of pHLIP ICG in vivo (.1,100 nm, 300 ms). Conclusion: SWIRFI sensitivity and ambient light resistance enabled continued tracer clearance tracking with unparalleled SNR and CNR values at video rates for tumor delineation (achieving a tumor-to-muscle ratio above 20). In total, we provide a direct precedent for the democratic translation of an ambient light resistant SWIRFI and pHLIP ICG ecosystem, which can instantly improve tumor resection.

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Section: Discussionmentioning

confidence: 99%

Ambient Light Resistant Shortwave Infrared Fluorescence Imaging for Preclinical Tumor Delineation via the pH Low-Insertion Peptide Conjugated to Indocyanine Green

Mc Larney,

Kim,

Roberts

et al. 2023

J Nucl Med

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“…Upon irradiation with 808 nm light, the local heat in tumor site only finally rises to ∼41.1 °C in the PBS-treated group (Figure B,C). Due to photobleaching of ICG, which frequently limits its observation time of NIR II fluorescent imaging and the exact photothermal conversion efficiency, the above findings may inevitably result in some errors. Due to the rough surface, even though the nanoplatform presents efficient tumor targeting, it is also heavily captured by the reticuloendothelial system, which ineluctably induces concerns about the potential systematic toxicity.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Photothermal and Chemodynamic Tumor Therapy by a Cu–Ca-Based Nanoplatform

Huang,

Wang,

et al. 2024

ACS Appl. Nano Mater.

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In this study, a fascinating nanoplatform, possessing fluorescent imaging, hyperthermia-amplified reactive oxygen species (ROS) generation, and calcium-overloaded comediated multimodal therapeutic effects toward breast malignancy, was innovatively constructed. Accordingly, engineering this accurate targeting theranostic scheme can be used for efficacious ablation of breast carcinoma. Because Cu and Ca ions can induce ROS generation and cytoplasmic overaccumulation, respectively, here, a Cu−Ca-based nanoplatform was designed by adsorbing indocyanine green (ICG) through the relatively high specific surface area and Arg-Gly-Asp peptide (RGD) decorating by covalent conjugation. Upon the internalization of breast tumor cells, biodegradation was triggered under an intracellular weak acid microenvironment; the released ICG molecules allowed for near-IR (NIR) II fluorescent imaging and photothermal production. Importantly, Cu ions in the nanoplatform guaranteed more efficient Fenton-like reaction-mediated chemodynamic therapy under NIR laser exposure. Meanwhile, the virus-like hollowed Cu−Ca (VhCu-Ca) nanoplatform created an artificial calcium-overloading stress in the cytoplasm. In vitro and in vivo studies have demonstrated that our proposed Cu−Ca-based nanoplatform integrated the advantages of breast tumor targeting, NIR II fluorescent imaging, and efficient ablation via synergistic treatments. Therefore, this multimode nanoplatform developed a facile and versatile strategy for the diagnosis and suppression of breast tumors.

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“…Ensuring laser triggering in combination with these shortened exposure times would reduce the overall exposure of the agent and thus reduce photobleaching effects. ( 57-60 ) A knock-on of this would be an extended surgical window and surgical imaging time.…”

Section: Discussionmentioning

confidence: 99%

Preclinical shortwave infrared tumor screening and resection via pHLIP ICG under ambient lighting conditions

Larney

Kim

Roberts

et al. 2022

Preprint

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There is a critical need to improve optical imaging that will lead to its widespread acceptance for routine clinical procedures. Shortwave infrared (SWIR, 900 to 1700nm) imaging has demonstrated clear advantages over visible and near infrared imaging (reduced autofluorescence with improved contrast, resolution, and sensitivity at tissue depth). Here we show that the previously reported compound, pH low insertion peptide (pHLIP) conjugated to indocyanine green (ICG, pHLIP ICG) currently in clinical trials, serves as an excellent candidate for SWIR imaging protocols. SWIRs increased sensitivity enabled preclinical tumor screening and resection at exposure times as low as 0.1 ms with acceptable signal to noise and contrast to noise ratios. Imaging was performed under ambient lighting conditions, and SWIRs sensitivity enabled an extended surgical resection window up to 96 hrs post injection in an orthotopic breast cancer mouse model. This work provides a direct precedent for the clinical translation of SWIR pHLIP ICG imaging for cancer resection.

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Role of photobleaching process of indocyanine green for killing neuroblastoma cells

Cited by 8 publications

References 21 publications

Ambient Light Resistant Shortwave Infrared Fluorescence Imaging for Preclinical Tumor Delineation via the pH Low-Insertion Peptide Conjugated to Indocyanine Green

Ambient Light Resistant Shortwave Infrared Fluorescence Imaging for Preclinical Tumor Delineation via the pH Low-Insertion Peptide Conjugated to Indocyanine Green

Enhanced Photothermal and Chemodynamic Tumor Therapy by a Cu–Ca-Based Nanoplatform

Preclinical shortwave infrared tumor screening and resection via pHLIP ICG under ambient lighting conditions

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