Role of PhoU in phosphate transport and alkaline phosphatase regulation

Muda, Marco; Rao, Narayana N.; Torriani, Annamaria

doi:10.1128/jb.174.24.8057-8064.1992

Cited by 68 publications

(64 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although phoU has not been observed in viruses, phosphate stress and acquisition genes (for example, pstS, phoA, phoH) are common in T4-like cyanophages isolated from low-phosphate waters (Sullivan et al, 2010). Here, phoU may enable rapid uptake of free phosphate for use in DNA synthesis (Muda et al, 1992), and adds to the paradigm that phosphate scavenging is critical to phosphate-intensive marine viral reproduction (Bratbak, 1993;Clasen and Elser, 2007).…”

Section: Co-localized With Viral Genes On Contigs (Includes Genes Idementioning

confidence: 99%

Depth-stratified functional and taxonomic niche specialization in the ‘core’ and ‘flexible’ Pacific Ocean Virome

2014

View full text Add to dashboard Cite

Microbes drive myriad ecosystem processes, and their viruses modulate microbial-driven processes through mortality, horizontal gene transfer, and metabolic reprogramming by viral-encoded auxiliary metabolic genes (AMGs). However, our knowledge of viral roles in the oceans is primarily limited to surface waters. Here we assess the depth distribution of protein clusters (PCs) in the first large-scale quantitative viral metagenomic data set that spans much of the pelagic depth continuum (the Pacific Ocean Virome; POV). This established ‘core’ (180 PCs; one-third new to science) and ‘flexible’ (423K PCs) community gene sets, including niche-defining genes in the latter (385 and 170 PCs are exclusive and core to the photic and aphotic zones, respectively). Taxonomic annotation suggested that tailed phages are ubiquitous, but not abundant (<5% of PCs) and revealed depth-related taxonomic patterns. Functional annotation, coupled with extensive analyses to document non-viral DNA contamination, uncovered 32 new AMGs (9 core, 20 photic and 3 aphotic) that introduce ways in which viruses manipulate infected host metabolism, and parallel depth-stratified host adaptations (for example, photic zone genes for iron–sulphur cluster modulation for phage production, and aphotic zone genes for high-pressure deep-sea survival). Finally, significant vertical flux of photic zone viruses to the deep sea was detected, which is critical for interpreting depth-related patterns in nature. Beyond the ecological advances outlined here, this catalog of viral core, flexible and niche-defining genes provides a resource for future investigation into the organization, function and evolution of microbial molecular networks to mechanistically understand and model viral roles in the biosphere.

show abstract

Section: Co-localized With Viral Genes On Contigs (Includes Genes Idementioning

confidence: 99%

Depth-stratified functional and taxonomic niche specialization in the ‘core’ and ‘flexible’ Pacific Ocean Virome

2014

View full text Add to dashboard Cite

show abstract

“…It is usually constitutively expressed and is dependent on the proton motive force. The high-affinity phosphate specific transport system is induced during phosphate starvation by the Pho regulon and is an ABC transporter (Muda et al, 1992;Nikata et al, 1996;Aguena et al, 2002;Gebhard et al, 2006). Therefore, in most environments with low phosphate availability, the high-affinity phosphate transport system is expected to be the primary pathway for phosphate introduction into cells.…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of phosphate limitation in Geobacteraceae during the bioremediation of a uranium-contaminated aquifer

et al. 2009

View full text Add to dashboard Cite

Nutrient limitation is an environmental stress that may reduce the effectiveness of bioremediation strategies, especially when the contaminants are organic compounds or when organic compounds are added to promote microbial activities such as metal reduction. Genes indicative of phosphatelimitation were identified by microarray analysis of chemostat cultures of Geobacter sulfureducens. This analysis revealed that genes in the pst-pho operon, which is associated with a high-affinity phosphate uptake system in other microorganisms, had significantly higher transcript abundance under phosphate-limiting conditions, with the genes pstB and phoU upregulated the most. Quantitative PCR analysis of pstB and phoU transcript levels in G. sulfurreducens grown in chemostats demonstrated that the expression of these genes increased when phosphate was removed from the culture medium. Transcripts of pstB and phoU within the subsurface Geobacter species predominating during an in situ uranium-bioremediation field experiment were more abundant than in chemostat cultures of G. sulfurreducens that were not limited for phosphate. Addition of phosphate to incubations of subsurface sediments did not stimulate dissimilatory metal reduction. The added phosphate was rapidly adsorbed onto the sediments. The results demonstrate that Geobacter species can effectively reduce U(VI) even when experiencing suboptimal phosphate concentrations and that increasing phosphate availability with phosphate additions is difficult to achieve because of the high reactivity of this compound. This transcript-based approach developed for diagnosing phosphate limitation should be applicable to assessing the potential need for additional phosphate in other bioremediation processes.

show abstract

“…These proteins share considerable identity with the products of the functionally related pstSCABU operon of Escherichia coli: BsuPstS (PstS of B. subtilis, 309 amino acids in length) exhibits 25 % identity with EcoPstS (PstS of E. coli); BsuPstC (300 amino acids) has 28 % identity with EcoPstC; BsuPstA (294 amino acids) has 28 % identity with EcoPstA; BsuPstBA (269 amino acids) and BsuPstBB (260 amino acids) both have 57 % identity with EcoPstB. The E. coli pst operon additionally includes phoU, encoding a regulatory component of the transporter that is not present in B. subtilis (Muda et al, 1992;Steed & Wanner, 1993).…”

Section: Introductionmentioning

confidence: 99%

Post-transcriptional regulation of the Bacillus subtilis pst operon encoding a phosphate-specific ABC transporter

et al. 2004

View full text Add to dashboard Cite

During phosphate starvation, Bacillus subtilis regulates genes in the PhoP regulon to reduce the cell's requirement for this essential substrate and to facilitate the recovery of inorganic phosphate from organic sources such as teichoic and nucleic acids. Among the proteins that are highly induced under these conditions is PstS, the phosphate-binding lipoprotein component of a high-affinity ABC-type phosphate transporter. PstS is encoded by the first gene in the pst operon, the other four members of which encode the integral membrane and cytoplasmic components of the transporter. The transcription of the pst operon was analysed using a combination of methods, including transcriptional reporter gene technology, Northern blotting and DNA arrays. It is shown that the primary transcript of the pst operon is processed differentially to maintain higher concentrations of PstS relative to other components of the transporter. The comparative studies have revealed limitations in the use of reporter gene technology for analysing the transcription of operons in which the messenger RNA transcript is differentially processed.

show abstract

Role of PhoU in phosphate transport and alkaline phosphatase regulation

Cited by 68 publications

References 36 publications

Depth-stratified functional and taxonomic niche specialization in the ‘core’ and ‘flexible’ Pacific Ocean Virome

Depth-stratified functional and taxonomic niche specialization in the ‘core’ and ‘flexible’ Pacific Ocean Virome

Molecular analysis of phosphate limitation in Geobacteraceae during the bioremediation of a uranium-contaminated aquifer

Post-transcriptional regulation of the Bacillus subtilis pst operon encoding a phosphate-specific ABC transporter

Contact Info

Product

Resources

About