Role of placenta-specific protein 1 in trophoblast invasion and migration

Chang, Wen-Lin; Yang, Qing; Zhang, Hui; Lin, Haiyan; Zhou, Zhi; Lu, Xiaoyin; Zhu, Cheng; Li-qun, Xue; Wang, Hongmei

doi:10.1530/rep-14-0052

Cited by 47 publications

(40 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA interference (RNAi) knockdown of PLAC1 in trophoblast-derived HTR8 cells resulted in significant ablation of both the invasive and migratory abilities of these cells, thus potentially implicating PLAC1 dysfunction in a variety of disorders including unexplained infertility, pre-term birth and preeclampsia [12].…”

Section: Plac1 Functionmentioning

confidence: 99%

Placenta-specific protein 1 (PLAC1) is a unique onco-fetal-placental protein and an underappreciated therapeutic target in cancer

Devor¹

2016

Integr Cancer Sci Therap

View full text Add to dashboard Cite

Placenta-specific protein 1 (PLAC1) is an X-linked gene whose expression is almost exclusively limited to placental trophoblasts. Its expression profile and evolutionary history suggest that it is important in the proper establishment and maintenance of a healthy placenta and a successful gestation throughout the Eutheria through promoting invasiveness, migration and proliferation of placental tissue. For the same reasons, PLAC1 expression has been found to be co-opted in a variety of cancers. For reasons less understood, it is also expressed in developing embryos. Thus, PLAC1 is the only known member of a class of proteins termed "onco-fetal-placental."

show abstract

Section: Plac1 Functionmentioning

confidence: 99%

Placenta-specific protein 1 (PLAC1) is a unique onco-fetal-placental protein and an underappreciated therapeutic target in cancer

Devor¹

2016

Integr Cancer Sci Therap

View full text Add to dashboard Cite

show abstract

“…Another study focusing on human trophoblasts have shown that PLAC1 is very important for the invasion of extravillous trophoblasts [12] and syncytialization [25]. Plac1 is abundantly expressed in mice, and is widely distributed in different trophoblast subpopulations.…”

Section: Discussionmentioning

confidence: 99%

“…The sections were fixed by neutral paraformaldehyde (PFA; pH 7.4) for 1 h at 4°C. ISH was performed as previously described [12]. Briefly, 2 µg/mL of DIG-labeled cRNA probe of Plac1 was used for hybridization at 60°C for 16 h. The sections were then counterstained by nuclear fast red solution (N3020, Sigma).…”

Section: Methodsmentioning

confidence: 99%

Plac1 Expression Pattern at the Mouse Fetomaternal Interface and Involvement in Trophoblast Differentiation

Wan

et al. 2017

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background/Aims: It is well known that Plac1 is a placenta-specific gene; however, its spatiotemporal expression pattern and exact role at t h e mouse fetomaternal interface r e m a i n s unclear. Methods: In situ hybridization (ISH) was used to localize the Plac1 mRNA at the mouse fetomaternal interface. A trophoblast stem cell (TS) differentiation model with Plac1 shRNA-overexpressing lentivirus was employed to investigate the possible role of Plac1 in placentation. Real-time RT-PCR was used to detect changes in gene expression. Results: Plac1 was exclusively expressed in the ectoplacental cone (EPC) as well as in 8.5 and 9.5 days post-coitum (dpc) embryos. Subsequently, Plac1 expression was abundant in the spongiotrophoblast layer and moderately in the labyrinth layer until 13.5 dpc, and declined thereafter. Interestingly, Plac1 was also expressed by secondary trophoblast giant cells and glycogen trophoblast cells, but not in primary trophoblast giant cells. Plac1 transcription was increased during the TS differentiation (P < 0.01), and knockdown of Plac1 significantly impaired TS differentiation. Conclusion: Plac1 is abundantly expressed at the fetomaternal interface and in all trophoblast subtypes except in primary trophoblast giant cells. Plac1 knockdown retarded the progress of TS differentiation, indicating that Plac1 is necessary for normal trophoblast differentiation into various trophoblast subpopulations.

show abstract

“…Frozen sections of 6 mm were sectioned by Leica CM1950 (Leica Microsystems, Wetzlar, Germany). ISH was performed as previously described with small modifications (Chang et al, 2014). DIG-labeled RNA probes of PAPPA2 with a concentration of 2 mg/mL were used for hybridization at 56°C for 16 h. The sections were directly photographed for antisense probe incubation.…”

Section: In Situ Hybridization (Ish)mentioning

confidence: 99%

“…Matrigel cell invasion and transwell cell migration assays were performed as previously described (Chang et al, 2014). In summary, HTR8/SVneo cells were transfected with PAPPA2-specific siRNA or the universal negative siRNA (NC).…”

Section: Matrigel Cell Invasion and Transwell Cell Migration Assaysmentioning

confidence: 99%

Expression of PAPPA2 in human fetomaternal interface and involvement in trophoblast invasion and migration

2016

View full text Add to dashboard Cite

ABSTRACT. Pregnancy-associated plasma protein-A 2 (PAPPA2) is a placental-enriched gene that is important for normal human placentation and defects in the gene can cause complications in pregnancy. Yet the exact expression pattern and role of PAPPA2 in the human fetomaternal interface are not clear. In this study, in situ hybridization (ISH) and immunohistochemistry (IHC) were employed to examine the spatial and temporal expression of PAPPA2 in the human fetomaternal interface. IHC results exhibited wide expression of PAPPA2 in the fetomaternal interface, with placental syncytiatrophoblast (STB) and extravillous trophoblast (EVT) showing strong expression and the cytotrophoblast (CTB) showing weak expression of PAPPA2. These results were confirmed by ISH. Quantitative reverse transcription- polymerase chain reaction and western blot showed the elevation of PAPPA2 in first trimester EVT differentiation and term CTB spontaneous syncytialization. PAPPA2-siRNA transfection significantly depressed the invasion and migration ability of a trophoblast cell line (HTR8/SVneo) in a transwell migration and Matrigel invasion model compared to a negative control siRNA (P < 0.05), also revealing that matrix metalloproteinase 9 (MMP9) secretion is downregulated. This was confirmed using a human first trimester placental villi explant culture model. Our results reveal the spatial and temporal expression of PAPPA2 in the human fetomaternal interface and show the positive regulatory role of PAPPA2 in human trophoblast invasion and migration through the secretion of MMP9.

show abstract

Role of placenta-specific protein 1 in trophoblast invasion and migration

Cited by 47 publications

References 41 publications

Placenta-specific protein 1 (PLAC1) is a unique onco-fetal-placental protein and an underappreciated therapeutic target in cancer

Placenta-specific protein 1 (PLAC1) is a unique onco-fetal-placental protein and an underappreciated therapeutic target in cancer

Plac1 Expression Pattern at the Mouse Fetomaternal Interface and Involvement in Trophoblast Differentiation

Expression of PAPPA2 in human fetomaternal interface and involvement in trophoblast invasion and migration

Contact Info

Product

Resources

About