Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of ‘Candidatus Phytoplasma solani’-inflicted bois noir disease of grapevine in Serbia

Kosovac, Andrea; Jakovljević, Miljana; Krstić, Oliver; Cvrković, Tatjana; Mitrović, Milana; Toševski, Ivo; Jović, Jelena

doi:10.1007/s10658-018-1553-1

Cited by 34 publications

(71 citation statements)

References 39 publications

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“…In the last few years, numerous studies revealed that the BN epidemiology is more complex than previously postulated, reporting new relevant plant reservoirs (Vitex agnus-castus L. and Crepis foetida L.) for the known CaPsol vectors, and identifying some of the unknown alternative vectors [7,8,12]. Here, a new BN epidemiological pattern (grapevine-Rq-weeds) related to St10 CaPsol strains and milder symptoms is proposed.…”

Section: Discussionmentioning

confidence: 87%

“…The cixiid Hyalesthes obsoletus Signoret, the main vector of BN [4], acquires CaPsol from its preferred host plants (Convolvulus arvensis L. and Urtica dioica L.), and occasionally transmits it to grapevine, a phytoplasma dead-end host [5,6]. Recently, typing of CaPsol strains using different molecular markers improved the knowledge of BN spreading, highlighting the crucial role of Vitex agnus-castus L. and Crepis foetida L. as the main CaPsol source plants for H. obsoletus [7,8], and proposing the involvement of other weeds and insects in BN epidemiology [9,10]. Moreover, combining field surveys, phytoplasma molecular characterization and transmission trials, Reptalus panzeri (Löw) was reported as a natural vector of CaPsol in Serbian vineyards [11], and another eight alternative vectors of CaPsol to grapevine (Aphrodes makarovi Zachvatkin, Dicranotropis hamata (Boheman), Dictyophara europaea (L.), Euscelis incisus (Kirschbaum), Euscelidius variegatus (Kirschbaum), Laodelphax striatella (Fallen), Philaenus spumarius (L.), and Psammotettix alienus/confinis (Dahlbom)) were identified in northern Italy [12].…”

Section: Introductionmentioning

confidence: 99%

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Proposal of A New Bois Noir Epidemiological Pattern Related to ‘Candidatus Phytoplasma Solani’ Strains Characterized by A Possible Moderate Virulence in Tuscany

et al. 2020

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Bois noir (BN), associated with ‘Candidatus Phytoplasma solani’ (CaPsol), is the most widespread disease of the grapevine yellows complex worldwide. In this work, BN epidemiology was investigated in a case study vineyard where an unusual CaPsol strain, previously detected only in other host plants, was found to be prevalent in grapevine. Experimental activities included: symptom observation; sampling of symptomatic vines, Auchenorrhyncha specimens, and weeds; molecular detection and typing of CaPsol strains; statistical analyses for determining possible relationships between CaPsol relative concentration, strain type, and symptom severity. Among insects, Reptalus quinquecostatus was the most abundant and was found to be highly infected by CaPsol, while Hyalesthes obsoletus, the main CaPsol vector, was not caught. Moreover, R. quinquecostatus harbored CaPsol strains carrying uniquely the stamp sequence variant St10, also identified as prevalent in vines and in the majority of weeds, and all the secY variants identified in the vineyard. Statistical analyses revealed that CaPsol strains carrying the St10 variant are not associated with severe symptoms, suggesting their possible moderate virulence. Based on such evidence, a new BN epidemiological pattern related to these CaPsol strains and involving grapevine, R. quinquecostatus, and/or weeds is proposed. Furthermore, the possible presence of other players (vectors and weeds) involved in CaPsol transmission to grapevines was highlighted.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Proposal of A New Bois Noir Epidemiological Pattern Related to ‘Candidatus Phytoplasma Solani’ Strains Characterized by A Possible Moderate Virulence in Tuscany

et al. 2020

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“…BN is associated with strains of ‘ Candidatus Phytoplasma solani’ (CaPsol) (subgroup 16SrXII-A), a phloem-limited cell wall-less bacterium of the Mollicutes class 1–5 . Hyalesthes obsoletus Signoret (Hemiptera, Cixiidae), a polyphagous insect feeding mainly on bindweed ( Convolvulus arvensis L.), nettle ( Urtica dioica L.), chaste tree ( Vitex agnus-castus L.), and stinking hawk’s beard ( Crepis foetida L.), is the principal vector of CaPsol strains to grapevine 6–8 . In the last years, the spreading of CaPsol in vineyards where H. obsoletus was absent suggested the existence of additional vectors.…”

Section: Introductionmentioning

confidence: 99%

“…A study conducted in Eastern Europe reported the direct epidemiological role of chaste trees as CaPsol source in the H. obsoletus -mediated transmission to grapevine 7 . Moreover, in the Balkan region, it was recently highlighted that H. obsoletus population related to stinking hawk’s beard can acquire CaPsol tuf -type b from this source plant and transmit it to grapevine 8 . A larger genetic diversity among CaPsol strains was described by molecular characterization of less conserved genes ( i.e ., secY , vmp1 , and stamp ) 17,18 .…”

Section: Introductionmentioning

confidence: 99%

Identification and ecology of alternative insect vectors of ‘Candidatus Phytoplasma solani’ to grapevine

Quaglino

Sanna

Moussa

et al. 2019

Sci Rep

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Bois noir, a disease of the grapevine yellows complex, is associated with ‘Candidatus Phytoplasma solani’ and transmitted to grapevines in open fields by the cixiids Hyalesthes obsoletus and Reptalus panzeri. In vine-growing areas where the population density of these vectors is low within the vineyard, the occurrence of bois noir implies the existence of alternative vectors. The aim of this study was to identify alternative vectors through screening of the Auchenorrhyncha community, phytoplasma typing by stamp gene sequence analyses, and transmission trials. During field activities, conducted in Northern Italy in a vineyard where the bois noir incidence was extremely high, nine potential alternative insect vectors were identified according to high abundance in the vineyard agro-ecosystem, high infection rate, and harbouring phytoplasma strains characterized by stamp gene sequence variants found also in symptomatic grapevines. Transmission trials coupled with molecular analyses showed that at least eight species (Aphrodes makarovi, Dicranotropis hamata, Dictyophara europaea, Euscelis incisus, Euscelidius variegatus, Laodelphax striatella, Philaenus spumarius, and Psammotettix alienus/confinis) are alternative vectors of ‘Candidatus Phytoplasma solani’ to grapevines. These novel findings highlight that bois noir epidemiology in vineyard agro-ecosystems is more complex than previously known, opening up new perspectives in the disease management.

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“…Recent studies have also reported on a further independent epidemiological cycle for the tuf b strain of ‘ Ca . P. solani’, detected in Crepis foetida and with transmission by H. obsoletus [ 25 ]. However, this study detected tuf b1 and two new genotypes that have not been reported previously that differ from tuf b1 in mutations.…”

Section: Discussionmentioning

confidence: 99%

Sequence Analysis of New Tuf Molecular Types of ‘Candidatus Phytoplasma Solani’ in Iranian Vineyards

et al. 2020

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Grapevine Bois noir (BN) is caused by ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’) and is one of the most important phytoplasma diseases in the Euro-Mediterranean viticultural areas. The epidemiology of BN can include grapevine as a plant host and is usually transmitted via sap-sucking insects that inhabit herbaceous host plants. Tracking the spread of ‘Ca. P. solani’ strains is of great help for the identification of plant reservoirs and insect vectors involved in local BN outbreaks. The molecular epidemiology of ‘Ca. P. solani’ is primarily based on sequence analysis of the tuf housekeeping gene (which encodes elongation factor Tu). In this study, molecular typing of tuf, through restriction fragment length polymorphism and sequencing, was carried out on grapevine samples from Iranian vineyards. According to the molecular characterization, three molecular types—tuf b1, tuf b5 and tuf b6—were found, with tuf b1 being the most prominent. These data provide further knowledge of tuf gene diversity and question the ecological role of such “minor” tuf types in Iranian vineyards, which have been detected only in grapevines.

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Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of ‘Candidatus Phytoplasma solani’-inflicted bois noir disease of grapevine in Serbia

Cited by 34 publications

References 39 publications

Proposal of A New Bois Noir Epidemiological Pattern Related to ‘Candidatus Phytoplasma Solani’ Strains Characterized by A Possible Moderate Virulence in Tuscany

Proposal of A New Bois Noir Epidemiological Pattern Related to ‘Candidatus Phytoplasma Solani’ Strains Characterized by A Possible Moderate Virulence in Tuscany

Identification and ecology of alternative insect vectors of ‘Candidatus Phytoplasma solani’ to grapevine

Sequence Analysis of New Tuf Molecular Types of ‘Candidatus Phytoplasma Solani’ in Iranian Vineyards

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