Role of plant volatiles in resistance of selected rice varieties to brown planthopper,Nilaparvata lugens (St�l) (Homoptera: Delphacidae)

An urgent need exists to identify more brown planthopper (Nilaparvata lugens Stål, BPH) resistance genes, which will allow the development of rice varieties with resistance to BPH to counteract the increased incidence of this pest species. Here, using bioinformatics and DNA sequencing approaches, we identified a novel BPH resistance gene, LOC_Os06g03240 (MSU LOCUS ID), from the rice variety Ptb33 in the interval between the markers RM19291 and RM8072 on the short arm of chromosome 6, where a gene for resistance to BPH was mapped by Jirapong Jairin et al. and renamed as “Bph32”. This gene encodes a unique short consensus repeat (SCR) domain protein. Sequence comparison revealed that the Bph32 gene shares 100% sequence identity with its allele in Oryza latifolia. The transgenic introgression of Bph32 into a susceptible rice variety significantly improved resistance to BPH. Expression analysis revealed that Bph32 was highly expressed in the leaf sheaths, where BPH primarily settles and feeds, at 2 and 24 h after BPH infestation, suggesting that Bph32 may inhibit feeding in BPH. Western blotting revealed the presence of Pph (Ptb33) and Tph (TN1) proteins using a Penta-His antibody, and both proteins were insoluble. This study provides information regarding a valuable gene for rice defence against insect pests.

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“…The rice variety Ptb33 showed a higher degree and a broader spectrum of BPH resistance than has been described previously24293358. E.R.…”

Section: Discussionmentioning

confidence: 60%

Bph32, a novel gene encoding an unknown SCR domain-containing protein, confers resistance against the brown planthopper in rice

Ren

Gao

et al. 2016

Sci Rep

133

131

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“…The candidate gene should be further confirmed A previous study reported that BPH resistance in rice cultivars carrying Bph3 exhibited antixenosis (Saxena and Okech 1985). This means, BPH had a tendency to move away from resistant plants after some time.…”

Section: Bph Resistance In Rht Is Associated With a Series Of Genesmentioning

confidence: 94%

Microarray analysis of broad-spectrum resistance derived from an indica cultivar Rathu Heenati

Wang

et al. 2011

Planta

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Rathu Heenati (RHT) is a Sri Lankan rice cultivar that carries a brown planthopper (BPH) resistance gene, Bph3, and shows broad-spectrum resistance to all four biotypes of BPH. The BPH-resistance loci in RHT has been studied extensively and assigned to four different rice chromosomes (3, 4, 6, and 10) by different research groups, but the gene has not been cloned previously. An Affymetrix rice genome array containing 48,564 japonica and 1,260 indica sequences was used to analyze the potential resistance-related genes on the four chromosomes by comparative analysis of the differentially expressed genes between resistant and susceptible rice cultivars exposed to BPH attack. The microarray results showed that at least 17 genes related to induced resistance and at least 193 genes related to constitutive resistance in RHT. On chromosome 3, the AOC4 was hypothesized to be the most important candidate gene. On chromosome 6, no valuable candidate resistance gene was identified in the Bph3 localization region. In the three Quantitative trait locus regions of chromosomes 3, 4, and 10, the numbers of constitutive and induced resistance-related genes found were 17, 26, and 12, respectively. The major probe on chromosome 10 represents a constitutive expression gene with a very high absolute fold-change of 2,588.82. The microarray analysis indicated that BPH resistance in RHT is probably controlled by a series of resistance-related genes. This study provides valuable information for cloning, functional analysis and marker-assisted breeding of these BPH resistance genes.

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“…BEHAVIORAL STUDIES INDICATE that chemoreception plays a part in host plant selection and acceptance and in the control of feeding in the rice brown plant hopper, Nilaparvata lugens (Stål) (Obata et al 1983, Saxena andOkech 1985). The Þne structure of putative chemoreceptors on the antennae (Aljunid and Anderson 1983) and proboscis (Foster et al 1983a(Foster et al , 1983b has been described together with the external features of sensilla on the tarsi (Lö sel and Goodman 1987).…”

mentioning

confidence: 97%

Electroantennogram Responses of <I>Nilaparvata lugens</I> (Homoptera: Delphacidae) to Plant Volatile Compounds

Youn

2002

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Electroantennogram (EAG) recordings were made from the plaque organ receptors of the rice brown plant hopper, Nilaparvata lugens (Stål). The receptors of the distal plaque organ of both male and female hoppers responded to the air from above chopped rice plants, and those of females gave a larger response. Female hoppers, tested with 27 plant volatiles, gave dose-related responses to 16 of the compounds, including a range of green leaf volatiles. The aliphatic aldehydes hexanal and (E)-2-hexenal elicited a larger response than a range of green leaf alcohols. Of the remaining compounds, acetophenone, benzaldehyde, ethyl and methyl benzoate, and amyl and isoamyl acetate were among the most effective in eliciting responses. Receptor recovery times showed some variation between compounds. A comparison of the dose-related response of 16 compounds in paraffin oil and in pentane showed that considerably larger responses were recorded when the highly volatile solvent pentane was used.

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Role of plant volatiles in resistance of selected rice varieties to brown planthopper,Nilaparvata lugens (St�l) (Homoptera: Delphacidae)

Cited by 53 publications

References 17 publications

Bph32, a novel gene encoding an unknown SCR domain-containing protein, confers resistance against the brown planthopper in rice

Bph32, a novel gene encoding an unknown SCR domain-containing protein, confers resistance against the brown planthopper in rice

Microarray analysis of broad-spectrum resistance derived from an indica cultivar Rathu Heenati

Electroantennogram Responses of <I>Nilaparvata lugens</I> (Homoptera: Delphacidae) to Plant Volatile Compounds

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