Role of Platelets in Tumor Cell Metastases

Karpatkin, Simon; Pearlstein, Edward; Salk, Peter L.; Yogeeswaran, Ganesa

doi:10.1111/j.1749-6632.1981.tb29726.x

Cited by 48 publications

(20 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of special importance, in this regard, are tumour cell-platelet interactions. It has been recognized for over 30 years that tumour metastasis is facilitated by blood platelets that form circulating coaggregates with tumour cells and contribute to the arrest of such emboli to the vasculature [11,12]. The most convincing evidence for a platelet role is the reduction of the number of experimental metastases formed in thrombocytopenic mice relative to normal animals [13].…”

Section: Introductionmentioning

confidence: 98%

Breast adenocarcinoma cell adhesion to the vascular subendothelium in whole blood and under flow conditions: Effects of α v β 3 and α IIb β 3 antagonists

Gomes

Vassy

Lebos

et al. 2004

Clin Exp Metastasis

View full text Add to dashboard Cite

Tumour cell adhesion to vascular extracellular matrix (ECM), an important step of metastatic progression, is promoted by platelets. The aim of our study was to investigate, in whole blood under venous and arterial shear conditions, the respective role of tumour cell a v b 3 and platelet a IIb b 3 integrins in MDA-MB-231 breast adenocarcinoma cell adhesion to human umbilical vein endothelial cell ECM. For that purpose, blood containing MDA-MB-231 cells was incubated with non-peptide antagonists specific for platelet a IIb b 3 (lamifiban) or tumour cell a v b 3 (SB-273005). At 300 s )1 , each antagonist used alone did not modify tumour cell adhesion, whereas, at 1500 s )1 , tumour cell adhesion was decreased by 25% in presence of lamifiban indicating a role of platelet a IIb b 3 at higher shear rate. However, a combination of SB-273005 and lamifiban, or c7E3 Fab (a potent inhibitor of both a IIb b 3 and a v b 3 ) inhibited tumour cell adhesion by 40-45%, at either shear rate applied, indicating a cooperation between these two integrins in MDA-MB-231 cell adhesion to ECM, as well as the participation of other adhesive receptors on tumour cells and/or platelets. Thus, efficient anti-metastatic therapy should target multiple receptors on tumour cells and platelets. Abbreviations: BSA -bovine serum albumin; DMEM -dulbecco modified eagle medium; ECM -extracellular matrix; EDTA -ethylenediaminetetraacetic acid; FITC -fluorescein isothiocyanate; HEPES -N-[2-hydroxyethyl]piperazine-N¢-[2-ethanesulfonic acid]; HUVEC -human umbilical vein endothelial cells; IgG -immunoglobulin;

show abstract

Section: Introductionmentioning

confidence: 98%

Breast adenocarcinoma cell adhesion to the vascular subendothelium in whole blood and under flow conditions: Effects of α v β 3 and α IIb β 3 antagonists

Gomes

Vassy

Lebos

et al. 2004

Clin Exp Metastasis

View full text Add to dashboard Cite

show abstract

“…In addition to its anticoagulant activity, heparin has other beneficial effects in cancer via different mechanisms. Of them, interference in tumor cell-platelet association by antiplatelet agents targeted to P-selectin-mediated interaction potentially inhibited both spontaneous and experimental metastasis in vivo (Karpatkin and Pearlstein 1981;Nash et al 2002;Varki and Varki 2002). However, the administration of heparin has a risk of bleeding from systemic anticoagulation.…”

Section: Introductionmentioning

confidence: 99%

Chemically modified heparin inhibits the in vitro adhesion of nonsmall cell lung cancer cells to P-selectin

Gao

Wei

Zheng

et al. 2005

J Cancer Res Clin Oncol

View full text Add to dashboard Cite

show abstract

“…One reason could be that most oral cancer metastasis occurs by lymphatic spread instead of through hematologic spread, and platelets appear to contribute to metastasis haematologically spread by their adhesive interaction with tumor cells via the adhesive proteins fibronectin and von Willebrand factor [15]. The ability of some tumor cells to aggregate platelets in vitro and their metastatic potential in vivo is well known [16]. It would be of interest to investigate whether oral SCC cell lines do have this ability.…”

Section: Discussionmentioning

confidence: 99%