Role of proliferative markers in breast lesions

Abstract

Study of proliferative markers help in the proper diagnosis of different breast lesions which lies in the grey zone on routine histopathology.

Morphometric and Histological Study of Breast Lesions with Special Reference to Proliferative Activity and Invasiveness

“…Thus, both of these benign lesions were found to be common below the age of onset of menopause. This finding corroborates with the findings of Manna et al [3]. They also found fibroadenoma as the most common breast tumor in the age group of 21-40 years.…”

“…Manna et al found PCNA LI(%) in the range of 5-7 % (mean 6 %) and 12-17 %(14.5 %) for fibroadenoma and fibrocystic disease with features of adenosis, respectively. They also showed that in the invasive areas PCNA LI (%) was 45-51 % with a mean of 49.3 % [3]. Thus, their findings corroborate with the present study.…”

“…Morphometric analysis was performed in terms of mean nuclear diameter (MND), mean nuclear area (MNA), mean cell diameter (MCD), mean cell area (MCA), and nuclear to cytoplasmic ratio (N/C ratio). For calculating for PCNA labeling index (PCNA LI%) at least 1000 nuclei were counted under 1000× magnification [3]. Regarding the interpretation of the result of p53 immunostaining, a tumor was considered as positive for p53 when it showed nuclear staining in more than 1 % of tumor cells [13].…”

“…Benign or malignant lesions do not pose any problem in histopathological diagnosis. But difficulty arises in differentiating between premalignant lesions and carcinoma in situ and minimally invasive carcinoma [3]. There are great differences so far in the prognosis of the lesions concerned.…”