Role of protein and fatty acid adsorption on platelet adhesion and aggregation at the blood–polymer interface

Kim, S. W.; Wisniewski, Stephen J.; Lee, E. S.; Winn, Maci

doi:10.1002/jbm.820110104

Cited by 37 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies reported in the literature on adsorption from plasma are those of Kim et al (19), Kochwa The majority of these studies used an approach similar to that taken in the present work, i. e. they focrrsed on the behavior of a few major proteins usually by trace h6sting. Kim et al (19) studied the adsorption of albu-in, fibrinogen and y-gtobulin on a group of hydrophobic surfac€s. Kinetics over a 3-hr period were "conventional" for all systerrrs.…”

Section: Discussionmentioning

confidence: 92%

“…However, comprehensive studies using plasma or blood ircelf as the adsorbate are less common. From data so far published using plasma as the bulk medium (18)(19)(20)(21) it appears that different behavior is to be expected in the more complex media. For saample the studies of Vroman et al (2L) suggest that the adsorbed layer composition will change with time in a plasma medium.…”

Section: !Ntroductionmentioning

confidence: 99%

See 1 more Smart Citation

Patterns of Adsorption of Proteins From Human Plasma Onto Foreign Surfaces

1982

View full text Add to dashboard Cite

SummaryThe deposition of proteins on blood-contacting surfaces is known to be a determining factor in subsequent thromboembolic events. The composition of the protein layers and how they change with time are unknown. To generate information relevant to these questions, the quantities of albumin, fibrinogen and IgG adsorbed on seven surfaces from human plasma as a function of time were measured using a tracelabeling method. Materials studied include several segmented polyether-urethanes, glass, siliconized glass (SG), polystyrene (PS) and polyethylene (PE).Fibrinogen, surprisingly, was not adsorbed from plasma to any of the hydrophilic surfaces. On PE and SG adsorption passed through an early maximum (before 2 min) then declined to near zero. Only on PS was adsorption substantial and constant with time. Albumin was also not detected on the hydrophilic materials, but was adsorbed substantially on the hydrophobic surfaces. IgG was detected on all surfaces, although in relatively low surface concentrations.These results suggest: 1. that the plasma itself interacts with initially adsorbed proteins, 2. that the role of fibrinogen adsorption in foreign-surface initiated thrombosis may need to be reevaluated and 3. that since the major plasma proteins are only minimally adsorbed, trace proteins may be important in blood-material interactions.

show abstract

Section: Discussionmentioning

confidence: 92%

Section: !Ntroductionmentioning

confidence: 99%

Patterns of Adsorption of Proteins From Human Plasma Onto Foreign Surfaces

1982

View full text Add to dashboard Cite

show abstract

“…Clearly, interactions between the implant surface and the host must be of predominant importance, and these events are likely initiated by surface--protein adsorption . Implantable biomaterials are spontaneously covered by a layer of host proteins within seconds after contact with body fluids (55)(56)(57)(58)(59). These adsorbed proteins gradually change conformation and become irreversibly adsorbed (24,31,32,(60)(61)(62).…”

Section: Discussionmentioning

confidence: 99%

Fibrin(ogen) mediates acute inflammatory responses to biomaterials.

Tang

Eaton

1993

The Journal of Experimental Medicine

424

360

View full text Add to dashboard Cite

SummaryAlthough "biocompatible" polymeric elastomers are generally nontoxic, nonimmunogenic, and chemically inert, implants made of these materials may trigger acute and chronic inflammatory responses. Early interactions between implants and inflammatory cells are probably mediated by a layer of host proteins on the material surface. To evaluate the importance of this protein layer, we studied acute inflammatory responses of mice to samples of polyester terephthalate film (PET) that were implanted intraperitoneally for short periods. Material preincubated with albumin is "passivated;' accumulating very few adherent neutrophils or macrophages, whereas uncoated or plasma-coated PET attracts large numbers ofphagocytes. Neither IgG adsorption nor surface complement activation is necessary for this acute inflammation; phagocyte accumulation on uncoated implants is normal in hypogammaglobulinemic mice and in severely hypocomplementemic mice. Rather, spontaneous adsorption of fibrinogen appears to be critical : (a) PET coated with serum or hypofibrinogenemic plasma attracts as few phagocytes as does albumin-coated material; (b) in contrast, PET preincubated with serum or hypofibrinogenemic plasma containing physiologic amounts of fibrinogen elicits "normal" phagocyte recruitment; (c) most importantly, hypofibrinogenemic mice do not mount an inflammatory response to implanted PET unless the material is coated with fibrinogen or the animals are injected with fibrinogen before implantation. Thus, spontaneous adsorption of fibrinogen appears to initiate the acute inflammatory response to an implanted polymer, suggesting an interesting nexus between two major iatrogenic effects of biomaterials : clotting and inflammation .

show abstract

“…The ratio of the plasma concentrations of albumin, y-globulin, and fibrinogen was 50:10:3; however, higher amounts of y-globulin and fibrinogen were used in the mixture as reported elsewhere (19). Albumin, y-globulin, and fibrinogen are the major plasma proteins and had been chosen for investigation of their role in the calcification process.…”

Section: In Vitro Calcification Experimentsmentioning

confidence: 99%

Glutaraldehyde Treated Bovine Pericardium: Changes in Calcification Due to Vitamins and Platelet Inhibitors

1997

View full text Add to dashboard Cite

Cardiovascular calcification, the formation of calcium phosphate deposits in cardiovascular tissue, is a common endstage phenomenon affecting a wide variety of bioprostheses. The purpose of the present paper is to study the possibility that some antiplatelet drugs (aspirin and persantine) and certain vitamins (vitamin C, vitamin B,, and vitamin E) and their combinations might prevent the mineralization of glutaraldehyde treated bovine pericardium (GABP) by modifying the pericardial surface. In this experimental protocol, we used Golomb and Wagner's (1991) in vitro model for studying GABP calcification and a diffusion cell with 2 compartments for evaluating the diffusion of calcium across the GABP. The results showed that a combination of aspirin and vitamins (0.5 mg% aspirin, 1.5 mg% vitamin C, 4 mg% vitamin B,, and 2 mg% vitamin E) in a metastable calcium phosphate solution not only reduced the transport of calcium ions through GABP, but along with the combinations of 0.5 mg% aspirin and 5 mg% persantine also produced significant reductions in GABP calcification. The exact mechanism of these changes in the calcification of GABP are still unknown. From these in vitro findings, it appears that a combined vitamin therapy with low doses of aspirin may be beneficial for platelet suppression and thereby prevent thrombosis. In addition, the vitamins may modify calcium transport and interfere with the adsorption at the surface, thus reducing GABP calcification. However, an important question that remains unanswered is whether this inhibitory effect would continue if the antiplatelet drugs and vitamins were discontinued. For the answer, more in vivo studies are needed to develop applications. Key Words: Calcification-Fibrinogen-Vitamins-Cellular involvement.Cardiac valve disease is frequently treated by prosthetic heart valve replacement. Calcification has limited the durability of bioprosthetic heart valves fabricated from glutaraldehyde pretreated porcine aortic valves or bovine pericardiums (1-3). The determinants of mineralization include factors related to both the host metabolism and the implant structure and chemistry. Schoen et al.(3) postulate that calcification in bioprosthetic heart valves probably result from the inability of valvular cells to compartmentalize their low calcium content relative to the extracellular environment. Unfortunately, the exact mechanism of induction and propagation of this drawback is not well defined, and thus the method to prevent the calcification is not yet clear.Basically 2 types of approaches have been tried to ~~~ reduce pericardial calcification. Implant modification to prevent calcification includes the use of pretreatment of implant surfaces with various agents like diphosphonate, sodium dodecyl sulfate (SDS), protamine sulfate, aluminum, ferric ions, 2-amino oleic acid, etc. (43) and the local polymeric delivery of some of these agents (6). Recently, we reported the in vitro and in vivo efficacy of prolonged release chitosan matrices containing protamine sulfate and fe...

show abstract

Role of protein and fatty acid adsorption on platelet adhesion and aggregation at the blood–polymer interface

Cited by 37 publications

References 15 publications

Patterns of Adsorption of Proteins From Human Plasma Onto Foreign Surfaces

Patterns of Adsorption of Proteins From Human Plasma Onto Foreign Surfaces

Fibrin(ogen) mediates acute inflammatory responses to biomaterials.

Glutaraldehyde Treated Bovine Pericardium: Changes in Calcification Due to Vitamins and Platelet Inhibitors

Contact Info

Product

Resources

About