Role of puerarin in the signalling of neuropathic pain mediated by P2X3 receptor of dorsal root ganglion neurons

Xu, Changshui; Xu, Wenyuan; Xu, Hong; Xiong, Wei; Gao, Yun; Li, Guilin; Liu, Shuangmei; Xie, Jinyan; Tu, Guihua; Peng, Haiying; Qiu, Shuyi; Liang, Shangdong

doi:10.1016/j.brainresbull.2011.10.007

Cited by 55 publications

(26 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mechanical nociceptive threshold was evaluated by observing withdrawal responses to mechanical stimulation using von Frey filaments (Stoelting, Wood Dale, IL, USA) [22][23][24]. The von Frey filament was used to apply a linearly increasing pressure, starting at 0.13 g and increasing until a withdrawal response occurred.…”

Section: Measurement Of the Mwtmentioning

confidence: 99%

“…The latency to hind paw withdrawal from a thermal stimulus was determined by exposing the plantar surface of the hind paw to radiant heat using the Thermal Paw Stimulation System (BME-410C, Tianjin, China) [22][23][24]. Rats were placed in a transparent, square, bottomless acrylic box (22 cm × 12 cm × 22 cm) on a glass plate under which a light was located.…”

Section: Measurement Of the Twlmentioning

confidence: 99%

“…The DRG were isolated immediately and rinsed in ice-cold phosphate-buffered saline (PBS) [24]. After dilution with sample buffer (250 mmol/L Tris-Cl, 200 mmol/L dithiothreitol, 10% sodium dodecyl sulfate (SDS), 0.5% bromophenol blue, and 50% glycerol) and heating to 95°C for 10 min, 20-μg samples of total protein were separated for P2X3 receptor analysis using 10% SDSpolyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membrane.…”

Section: Western Blottingmentioning

confidence: 99%

See 2 more Smart Citations

Nanoparticle-encapsulated emodin decreases diabetic neuropathic pain probably via a mechanism involving P2X3 receptor in the dorsal root ganglia

Xuan

Zhao

et al. 2017

Purinergic Signalling

Self Cite

View full text Add to dashboard Cite

Diabetic peripheral neuropathy (DPN) is the most common complication of diabetes mellitus (DM). More than 90% of all cases of DM belong to type 2 diabetes mellitus (T2DM). Emodin is the main active component of Radix et rhizoma rhei and has anti-bacterial, anti-viral, anti-ulcerogenic, anti-inflammatory, and anti-cancer effects. Nanoparticle encapsulation of drugs is beneficial for drug targeting and bioavailability as well as for lowering drug toxicity side effects. The aim of this study was to investigate the effects of nanoparticleencapsulated emodin (nano emodin) on diabetic neuropathic pain (DNP) mediated by the Purin 2X3 (P2X3) receptor in the dorsal root ganglia (DRG). Mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) values in T2DM rats were lower than those of control rats. MWT and TWL in T2DM rats treated with nano emodin were higher compared with those in T2DM rats. Expression levels of P2X3 protein and messenger RNA (mRNA) in the DRG of T2DM rats were higher than those of controls, while levels in T2DM rats treated with nano emodin were significantly lower than those of the T2DM rats. Phosphorylation and activation of ERK1/2 in the T2DM DRG were decreased by nano emodin treatment. Nano emodin significantly inhibited currents activated by the P2X3 agonist α,β-meATP in HEK293 cells transfected with the P2X3 receptor. Therefore, nano emodin treatment may relieve DNP by decreasing excitatory transmission mediated by the DRG P2X3 receptor in T2DM rats.

show abstract

Section: Measurement Of the Mwtmentioning

confidence: 99%

Section: Measurement Of the Twlmentioning

confidence: 99%

Section: Western Blottingmentioning

confidence: 99%

See 1 more Smart Citation

Nanoparticle-encapsulated emodin decreases diabetic neuropathic pain probably via a mechanism involving P2X3 receptor in the dorsal root ganglia

Xuan

Zhao

et al. 2017

Purinergic Signalling

Self Cite

View full text Add to dashboard Cite

show abstract

“…The latency to hind paw withdrawal from a thermal stimulus was determined by exposing the plantar surface of the hind paw to radiant heat using the Thermal Paw Stimulation System (BME-410C, Tianjin) [44,47,48]. Rats were placed in a transparent, square, bottomless acrylic box (22 cm × 12 cm × 22 cm) on a glass plate under which a light was located.…”

Section: Thermal Hyperalgesiamentioning

confidence: 99%

“…The mechanical nociceptive threshold was evaluated by observing the withdrawal responses to mechanical stimulation using von Frey filaments (Stoelting, Wood Dale, IL, USA) [44,47,48]. The von Frey filament was used to apply a linearly increasing pressure once, starting with 0.13 g and continuing until a withdrawal response occurred or the force reached 20.1 g (the cutoff value).…”

Section: Mechanical Hyperalgesiamentioning

confidence: 99%

LncRNA uc.48+ is involved in diabetic neuropathic pain mediated by the P2X3 receptor in the dorsal root ganglia

Wang

Zou

et al. 2015

Purinergic Signalling

Self Cite

View full text Add to dashboard Cite

Some long non-coding RNAs (lncRNAs) participate in physiological processes that maintain cellular and tissue homeostasis, and thus, the dysregulated expression of lncRNAs is involved in the onset and progression of many pathological conditions. Research has indicated that the genetic knockout of some lncRNAs in mice resulted in peri-or postnatal lethality or developmental defects. Diabetes mellitus (DM) is a major cause of peripheral neuropathy. Our studies showed that the expression levels of lncRNA uc.48+ in the diabetic rat dorsal root ganglia (DRG) and the DM patients' serum samples were increased. It suggested that lncRNA uc.48+ was involved in the pathophysiological process of DM. The aim of this study was to investigate the effects of lncRNA uc.48+ small interfering RNA (siRNA) on diabetic neuropathic pain (DNP) mediated by the P2X 3 receptor in the DRG. The values of the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured by the von Frey test and Hargreaves' test, respectively. The levels of P2X 3 protein and messenger RNA (mRNA) in the DRG were detected by reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, and western blotting. The experiments showed that the MWT and TWL values in DM rats were lower than those in the control rats. The MWT and TWL values in DM rats treated with lncRNA uc.48+ siRNA were increased compared to those in DM rats, but there was no significant difference between the DM rat group and the DM + scramble siRNA group. The levels of P2X 3 protein and mRNA in the DM DRG were higher than those in the control, while the levels of P2X 3 protein and mRNA in the DG of DM rats treated with uc.48+ siRNA were significantly decreased compared to those in DM rats. The expression levels of TNF-α in the DRG of DM rats treated with uc.48+ siRNA were significantly decreased compared to those in the DM group. The phosphorylation and activation of ERK1/2 in the DM DRG were decreased by uc.48+ siRNA treatment. Therefore, uc.48+ siRNA treatment may alleviate the DNP by inhibiting the excitatory transmission mediated by the P2X 3 receptor in DRG.

show abstract

Pregabalin Alters Nociceptive Behavior and Expression Level of P2X3 Receptor in the Spinal Dorsal Horn in a Rat Model Induced by Chronic Compression of the Dorsal Root Ganglion

Zhang

et al. 2013

The Anatomical Record

View full text Add to dashboard Cite

P2X3 receptors are present in the spinal dorsal horn (SDH) and play an essential role in the regulation of nociception and pain. Pregabalin (PGB) has been used as a new antiepileptic drug in the treatment of neuropathic pain. However, it is unclear whether PGB-induced analgesia was associated with the P2X3 receptor in SDH. Here, rats were randomly divided into four groups (n 5 12 per group), including 2 sham operation groups, which was treated by normal saline (Sham 1 NS group) or PGB (Sham 1 PGB group), other 2 groups with chronic compression of the dorsal root ganglion, a normal saline-treated CCD group (CCD1NS group), and a PGB-treated CCD group (CCD 1 PGB group). A rat model of neuropathic pain was used by compressing the right L4 and L5 dorsal root ganglia. Each group was evaluated using the mechanical withdrawal threshold (MWT). The mRNA and protein levels of the P2X3 receptor in the ipsilateral SDH were measured by RT-PCR, western blot, and immunofluorescence on 14 day after CCD operation. CCD rats showed the highest mechanical hyperalgesia and the lowest pain threshold in the four groups. Simultaneously, CCD rats showed higher P2X3 mRNA and protein expression in ipsilateral side of the SDH than the sham operation rats. However, the MWT was increased and expression of P2X3 mRNA and protein in the ipsilateral SDH in CCD rats was decreased 3 days after PGB treatment. Thus, PGB may partially reverse mechanical hyperalgesia in CCD rats by inhibiting P2X3 receptor expression in the ipsilateral SDH. Anat Rec, 296:1907Rec, 296: -1912Rec, 296: , 2013. V C 2013 Wiley Periodicals, Inc.Abbreviations used: CCD 5 chronic compression of dorsal root ganglion; cDNA 5 complementary deoxyribonucleic acid; DRG 5 dorsal root ganglion; L 5 lumbar segment; mRNA 5 messenger ribonucleic acid; MWT 5 mechanical withdrawal threshold; NS 5 normal saline; PGB 5 pregabalin; RT-PCR 5 reverse transcription-polymerase chain reaction; S 5 sacral segment; SDH 5 spinal dorsal horn.

show abstract

Role of puerarin in the signalling of neuropathic pain mediated by P2X3 receptor of dorsal root ganglion neurons

Cited by 55 publications

References 51 publications

Nanoparticle-encapsulated emodin decreases diabetic neuropathic pain probably via a mechanism involving P2X3 receptor in the dorsal root ganglia

Nanoparticle-encapsulated emodin decreases diabetic neuropathic pain probably via a mechanism involving P2X3 receptor in the dorsal root ganglia

LncRNA uc.48+ is involved in diabetic neuropathic pain mediated by the P2X3 receptor in the dorsal root ganglia

Pregabalin Alters Nociceptive Behavior and Expression Level of P2X3 Receptor in the Spinal Dorsal Horn in a Rat Model Induced by Chronic Compression of the Dorsal Root Ganglion

Contact Info

Product

Resources

About