Role of regeneration in tissue repairing and therapies

Upadhyay, Ravi Kant

doi:10.7243/2050-1218-4-1

Cited by 11 publications

(8 citation statements)

References 156 publications

(218 reference statements)

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“…Stem cells are present in various organs and tissues of the body. They possess regenerative potential and play a major role in repairing damaged tissues in the body throughout the span of the human life 1 2 . This has generated a great deal of interest in adult stem cells (ASCs), particularly since, unlike embryonic stem cells (ESCs), the use of ASCs does not pose moral and ethical dilemmas.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Beeravolu

McKee

Alamri

et al. 2017

JoVE

109

106

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The human umbilical cord (UC) and placenta are non-invasive, primitive and abundant sources of mesenchymal stromal cells (MSCs) that have increasingly gained attention because they do not pose any ethical or moral concerns. Current methods to isolate MSCs from UC yield low amounts of cells with variable proliferation potentials. Since UC is an anatomically-complex organ, differences in MSC properties may be due to the differences in the anatomical regions of their isolation. In this study, we first dissected the cord/placenta samples into three discrete anatomical regions: UC, cord-placenta junction (CPJ), and fetal placenta (FP). Second, two distinct zones, cord lining (CL) and Wharton's jelly (WJ), were separated. The explant culture technique was then used to isolate cells from the four sources. The time required for the primary culture of cells from the explants varied depending on the source of the tissue. Outgrowth of the cells occurred within 3 - 4 days of the CPJ explants, whereas growth was observed after 7 - 10 days and 11 - 14 days from CL/WJ and FP explants, respectively. The isolated cells were adherent to plastic and displayed fibroblastoid morphology and surface markers, such as CD29, CD44, CD73, CD90, and CD105, similarly to bone marrow (BM)-derived MSCs. However, the colony-forming efficiency of the cells varied, with CPJ-MSCs and WJ-MSCs showing higher efficiency than BM-MSCs. MSCs from all four sources differentiated into adipogenic, chondrogenic, and osteogenic lineages, indicating that they were multipotent. CPJ-MSCs differentiated more efficiently in comparison to other MSC sources. These results suggest that the CPJ is the most potent anatomical region and yields a higher number of cells, with greater proliferation and self-renewal capacities in vitro. In conclusion, the comparative analysis of the MSCs from the four sources indicated that CPJ is a more promising source of MSCs for cell therapy, regenerative medicine, and tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Beeravolu

McKee

Alamri

et al. 2017

JoVE

109

106

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“…ASCs have been proven to efficiently allow stimulation of cell-differentiation, immune-modulation, peripheral nerve repair and angiogenesis (4). In a recent study, ASCs have become powerful tools to improve wound healing and attenuate scar formation by promoting cell migration, angiogenesis and a possible regenerative rather than fibrotic microenvironment at the wound site (5).…”

Section: Introductionmentioning

confidence: 99%

Adipose-derived stem cell conditioned medium attenuates cisplatin-triggered apoptosis in tongue squamous cell carcinoma

Chiu

Yang

Hsu³

et al. 2017

Oncol Rep

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Autologous fat grafting procedures have noted a markedly increased frequency, not only for cosmetic purposes, but also for deformities after head and neck cancer and breast cancer surgery. Carcinogenesis is always a major concern in cell therapy-related issues. However, there is no literature discussing this issue in head and neck squamous cell carcinoma patients. To evaluate the interaction of tongue cancer cells and adipose-derived stem cells, we performed a series of in vitro experiments. Our results demonstrated that cisplatin significantly reduced the viabilities of SCC‑25 and CAL‑27 cells in a concentration-dependent manner, but it had low cytotoxicity in cisplatin-resistant CAL‑27 (CAR) cells. There was no significant difference in terms of viability among the SCC‑25, CAL‑27, and CAR cells in the adipose-derived stem cell conditioned medium and control groups. There was also no significant difference in terms of cell migration as determined by wound healing assay of SCC‑25, CAL‑27, and CAR cells between the adipose-derived stem cell conditioned medium treatment and control treatment. Importantly, the adipose-derived stem cell conditioned medium attenuated cisplatin-triggered cell death in the SCC‑25 and CAL‑27 cells. Moreover, adipose-derived stem cell conditioned medium markedly inhibited cisplatin-induced apoptotic cell death (sub‑G1 phase) in the CAL‑27 cells. Western blot analyses indicated that cisplatin-induced reductions in pro‑caspase‑3, pro‑caspase‑9, phospho-BAD, phospho-IGF-1R, phospho-AKT, and phospho-ERK in CAL‑27 cells were reversed by adipose-derived stem cell conditioned medium supplement. Taken together, we provide evidence that adipose-derived stem cell conditioned medium protects CAL‑27 cells from cisplatin-induced cell death, possibly through upregulation of the IGF-1R/AKT/ERK signaling pathway.

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“…The use of polysaccharide‐based hydrogels in drug delivery and tissue engineering was recently reviewed . The articles provide an excellent source for further information on biological and medical applications.…”

Section: Properties and Applicationsmentioning

confidence: 99%

Furfuryl‐ and Maleimido Polysaccharides: Synthetic Strategies Toward Functional Biomaterials

Elschner

Obst

Heinze

2018

Macromolecular Bioscience

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In context with facile and efficient syntheses of functional polymeric materials, the combination of polysaccharides and functional moieties based on renewable resources is a sustainable and valuable approach. This review presents alternatives to prominent click reactions utilizing biopolymer derivatives with furfuryl and maleimide groups. On the one hand, the cross‐linking by Diels–Alder reaction of these polymers enables the synthesis of novel materials in the fields of self‐healing polymers, tissue engineering, and drug delivery. On the other hand, thiol‐ene click reactions allow their conjugation to complex (bio)molecules. Different synthetic strategies are reviewed and the applicability of functional materials is evaluated.

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Role of regeneration in tissue repairing and therapies

Cited by 11 publications

References 156 publications

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Adipose-derived stem cell conditioned medium attenuates cisplatin-triggered apoptosis in tongue squamous cell carcinoma

Furfuryl‐ and Maleimido Polysaccharides: Synthetic Strategies Toward Functional Biomaterials

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