Role of roscovitine and IBMX on kinetics of nuclear and cytoplasmic maturation of bovine oocytes in vitro

Barretto, L. S. S.; Castro, V. S. D. Caiado; Garcia, J. M.; Mingoti, Gisele Zoccal

doi:10.1016/j.anireprosci.2006.06.001

Cited by 28 publications

(23 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…IBMX is a nonspecific inhibitor of phosphodiesterases that prevents meiotic resumption by promoting the persistence of the perinuclear microtubule network (Alexandre et al 1989) and by delaying the progression of nuclear maturation, without affecting cytoplasmic maturation (Barretto et al 2007). Following the addition of IBMX to the medium, we observed a significant increase in the frequencies of central and intermediate GVs and a significant decrease in peripheral GVs (P!0.05; (Zuccotti et al 1998, Inoue et al 2008, in vitro fertilized MII NSN oocytes cease their development at the two-cell stage.…”

Section: Resultsmentioning

confidence: 99%

The position of the germinal vesicle and the chromatin organization together provide a marker of the developmental competence of mouse antral oocytes

Bellone¹,

Zuccotti²,

Redi³

et al. 2009

Reproduction

View full text Add to dashboard Cite

Based on their chromatin organization, antral oocytes can be classified into two classes, namely surrounded nucleolus (SN, chromatin forms a ring around the nucleolus), and not surrounded nucleolus (NSN, chromatin has a diffuse pattern). Oocytes of both classes are capable of meiotic resumption, but while SN oocytes, following fertilization, develop to term, NSN oocytes never develop beyond the two-cell stage. A recent study has shown that the position of the germinal vesicle (GV) can be used as a morphological marker predictive of oocyte meiotic competence, i.e. oocytes with a central GV have a higher meiotic competence than oocytes with an eccentric GV. In the present study, we have associated both markers with the aim of identifying, with more accuracy, the oocytes' developmental competence. Following their isolation, antral oocytes were classified on the basis of both SN and NSN chromatin configuration and their GV position, matured to metaphase II and fertilized in vitro. We demonstrated that the position of the GV is a good marker to predict the oocytes' developmental competence, but only when associated with the observation of the chromatin organization.

show abstract

Section: Resultsmentioning

confidence: 99%

The position of the germinal vesicle and the chromatin organization together provide a marker of the developmental competence of mouse antral oocytes

Bellone¹,

Zuccotti²,

Redi³

et al. 2009

Reproduction

View full text Add to dashboard Cite

show abstract

“…However, several studies have recently demonstrated that prematuration of bovine oocytes in simple medium did not improve oocyte competence, but also did not cause major structural alterations in oocytes and embryos (Barretto et al, 2007;Adona et al, 2008). Then, it can be supposed that the addition of stimulatory components to the prematuration culture medium, such as hormones, could create a more suitable environment for promoting the developmental potential of the oocytes (Ponderato et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, previous results from our laboratory have demonstrated that prematuration of bovine oocytes in similar culture conditions did not impair oocyte cytoplasmic maturation, assessed by the analysis of CG repositioning (Barretto et al, 2007). Then, acquisition of developmental competence was assessed.…”

Section: Discussionmentioning

confidence: 99%

Meiotic inhibition of bovine oocytes in medium supplemented with a serum replacer and hormones: effects on meiosis progression and developmental capacity

Barretto

Castro

Garcia

et al. 2010

Zygote

View full text Add to dashboard Cite

SummaryAiming to improve the developmental competence of bovine oocytes during meiotic block, this study evaluated the effects of a serum replacer (Knockout SR R ) and hormones (gonadotropins and estradiol) supplementation of prematuration medium (TCM119 with 0.5 mM IBMX [IBMX group] or 25 μM roscovitine [ROSC group]) on the kinetics of oocyte nuclear maturation and embryo development. Most IBMX and ROSC oocytes prematured for 8 h culture remained in the GV stage (70.3% and 73.1%, respectively; p > 0.05) similar to Control 8 h (63.5%) and to control immature oocytes (Control 0 h, 92.5%). After prematuration for 16 h, no oocytes remained in the GV stage at similar rates to those recently aspirated (p < 0.05); GV rates in ROSC (32.4%) were higher (p < 0.05) than in the Control 16 h group (8.6%), but similar (p > 0.05) to IBMX (9.7%). After in vitro maturation (IMV) for 24 h, metaphase II (MII) rates for oocytes prematured during 8 h were similar (p > 0.05) between control and treatments (65.0-71.7%). Similarly, MII rates oocytes prematured during 16 h were similar (p > 0.05) between all groups (45.9-60.4%). Cleavage rates (67.8-78.2%), embryonic development in day-7 (25.0-35.6%) and hatching rates in day-8 (2.5-11.3%) oocytes blocked during 8 h were similar for all groups (p > 0.05).Results indicate that addition of Knockout SR R and hormones to meiotic block culture with IBMX and roscovitine negatively affected meiotic arrest, but did not impair oocyte nuclear maturation and acquisition of developmental competence.

show abstract

“…Germinal vesicle (GV) oocytes were isolated from ovaries of untreated mice into M2 medium [36] supplemented with 1 μM milrinone (Sigma) to prevent resumption of meiosis and maintain the oocytes at the GV stage [37]. Oocytes arrested at metaphase of the second meiotic division (MII): Female mice were injected with 7 IU of hCG, 48 h after administration of 5 IU PMSG.…”

Section: Oocytesmentioning

confidence: 99%

De novo synthesis of protein phosphatase 1A, magnesium dependent, alpha isoform (PPM1A) during oocyte maturation

Chuderland

Dvashi

Kaplan-Kraicer

et al. 2012

Cellular and Molecular Biology Letters

View full text Add to dashboard Cite

Oocyte maturation in mammals is a multiple-stage process that generates fertilizable oocytes. Ovarian oocytes are arrested at prophase of the first meiotic division characterized by the presence of a germinal vesicle. Towards ovulation, the oocytes resume meiosis and proceed to the second metaphase in a process known as maturation; they undergo nuclear and cytoplasmic changes that are accompanied by translation and degradation of mRNA. Protein phosphatase 1A, magnesium dependent, alpha isoform (PPM1A), which belongs to the metal-dependent serine/threonine protein phosphatase family, is highly conserved during evolution. PPM1A plays a significant role in many cellular functions such as cell cycle progression, apoptosis and cellular differentiation. It works through diverse signaling pathways, including p38 MAP kinase JNK and transforming growth factor beta (TGF-β). Herein we report that PPM1A is expressed in mouse oocytes and that its mRNA level rises during oocyte maturation. Using quantitative real-time polymerase chain reaction (qPCR) and western blot analysis, we found that PPM1A mRNA is synthesized at the beginning of the maturation process and remains elevated in the mature oocytes, promoting the accumulation of PPM1A

show abstract

Role of roscovitine and IBMX on kinetics of nuclear and cytoplasmic maturation of bovine oocytes in vitro

Cited by 28 publications

References 10 publications

The position of the germinal vesicle and the chromatin organization together provide a marker of the developmental competence of mouse antral oocytes

The position of the germinal vesicle and the chromatin organization together provide a marker of the developmental competence of mouse antral oocytes

Meiotic inhibition of bovine oocytes in medium supplemented with a serum replacer and hormones: effects on meiosis progression and developmental capacity

De novo synthesis of protein phosphatase 1A, magnesium dependent, alpha isoform (PPM1A) during oocyte maturation

Contact Info

Product

Resources

About