Role of secretome mesenchymal stem cells as immunomodulator in periodontal diseases

Sari, Desi Sandra; Maduratna, Ernie; Bumi, Candra; Sudiana, I Ketut; Rantam, Fedik Abdul

doi:10.15562/jdmfs.v6i3.989

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…Furthermore, the presence of growth factors in the DPMSCS can accelerate wound healing by inducing inflammatory mediators such as interleukin-1 (IL-1), Interleukin-6 (IL-6), and prostaglandin E2 (PGE2) in vitro, reducing the initial inflammatory reaction in vivo [25,26].…”

Section: Discussionmentioning

confidence: 99%

“…the induction of T cells and macrophages. The paracrine effects of MSCs are critical for maintaining epithelial cell integrity and promoting angiogenesis through their ability to regulate cell proliferation and differentiation [25,[36][37][38]. This study's results were only limited to traumatic ulcer animal model using Wistar rats (R. novergicus).…”

Section: Discussionmentioning

confidence: 99%

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Accelerating Wound Healing of Traumatic Ulcer with Topical Application of Dental Pulp Mesenchymal Stem Cell Secretome and Robusta Green Coffee Bean Extract Combination in vivo

Ichi,

Triwahyuni,

Sari

2023

Pesqui. Bras. Odontopediatria Clín. Integr.

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Objective: To investigate the ability of a combination dental pulp mesenchymal stem cell secretome (DPMSCS), robusta green coffee bean extract (RGCBE), and Carboxymethylcellulose-Natrium (CMC-Na) in a Wistar rats model of traumatic ulcers. Material and Methods: Twenty-eight young, male, healthy Wistar rats (Rattus norvegicus) were divided into seven groups randomly: Group K0, group K1-3 (traumatic ulcer rats that received CMC-Na gel for three days), group K1-7 (traumatic ulcer rats that received CMC-Na gel for seven days), group K2-3 (traumatic ulcer rats that received RGCBE for three days), group K2-7 (traumatic ulcer rats that received RGCBE for seven days), group K3-3 (traumatic ulcer rats that received DPMSCS for three days), and group K3-7 (traumatic ulcer rats that received DPMSCS for seven days). An ulcer was made with an amalgam stopper on the right buccal mucosa of the rats. DPMSCS 50% gel was applied to the ulcer on the left buccal mucosa. The ulcer diameter was measured on day 3 and day 7. Results: There was a significant difference in the diameter of the ulcer, the number of neutrophils, and fibroblasts in the treatment group compared to the control group on day 7. Conclusion: A combination of DPMSCS and RGCBE 50% accelerates traumatic ulcer wound healing by lowering ulcer diameter, decreasing neutrophil counts, and increasing fibroblast proliferation in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Accelerating Wound Healing of Traumatic Ulcer with Topical Application of Dental Pulp Mesenchymal Stem Cell Secretome and Robusta Green Coffee Bean Extract Combination in vivo

Ichi,

Triwahyuni,

Sari

2023

Pesqui. Bras. Odontopediatria Clín. Integr.

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“…6 Studies have shown the immunomodulatory effect of MSCs. 7 However, there is a lack of information regarding the effect of secretomes derived from human umbilical cord MSCs (UCMSCs) in vivo. Therefore, this study aimed to investigate the immunomodulatory activity of secretomes.…”

Section: Introductionmentioning

confidence: 99%

In vivo immunomodulatory effect and safety of MSC-derived secretome

et al. 2023

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Background: Secretomes have been gaining interest in treating several diseases due to their pharmaceutical effects, such as the immunomodulatory effect. This study aimed to determine the immunomodulatory effect of secretomes derived from human umbilical cord mesenchymal stem cells (MSCs) and their safety. Methods: We conducted an in vivo immunomodulatory study using a carbon clearance assay. The safety of single-dose administration of secretome was done using fixed-dose methods of acute toxicity test. Results: The phagocytic index was higher in mice treated with secretome than in untreated mice. The acute toxicity study also showed that the administration of secretome derived from human umbilical cord MSCs did not change the mice’s body weight, physical examination results, organ weight, and gross anatomy examination. Conclusions: This study presents the potential of secretome derived from MSCs as a safe immunomodulatory agent.

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Characteristic Differences between Freeze-dried Tenogenic Secretomes and Freeze-dried Crude Secretomes of Mesenchymal Stem Cells under Hypoxic Conditions

Kresnadi,

Edward,

Khang

et al. 2024

Biomolecular and Health Science Journal

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Introduction: Tendons that are crucial for movement are struggling with self-healing. Regenerative medicine has been using mesenchymal stem cells (MSCs) and their secretomes, and it offers promise. These secretomes, rich in growth factors, aid tissue healing. Tenogenic secretomes target tendon-specific healing, while crude ones provide a broader view of MSC signaling. The study aims to enhance targeted interventions in tendon regeneration and improve the quality of life for those with tendon-related issues. Methods: This laboratory study compares freeze-dried tenogenic and crude secretomes of MSCs under hypoxic conditions for regenerative potential. Stem cells from adipose tissue undergo isolation, culture, and multiplication then treated in hypoxic conditions. Characterization, Interleukin-6 (IL-6) and Interleukin-10 (IL-10) analysis with enzyme-linked immunosorbent assay (ELISA) follow. The results, including organoleptic, pH, and scanning electron microscope assessments, were presented descriptively. Statistical analysis will enhance understanding. Results: Physical characteristics (odor, color, and pH) are similar in both groups. Tenogenic secretome has a more concave surface, potentially aiding cell migration compared to the crude secretome. There were significant differences in cytokine levels (P = 0.001). IL-6 levels expressed from tenogenic secretome were 10.2 ± 2.432 pg/mL compared to the crude one which 18.3 ± 3.175 pg/mL. Levels of IL-10 expressed from tenogenic and crude secretomes were 309.2 ± 50.973 pg/mL and 200.1 ± 32.030, respectively. Conclusion: The tenogenic secretome and crude secretome exhibit no differences in physical characteristics but differ in surface morphology. The concave surface of the tenogenic secretome facilitates cell migration more than the crude secretome. Under hypoxic conditions, the tenogenic secretome shows lower pro-inflammatory (IL-6) and higher anti-inflammatory (IL-10) effects than the crude secretome.

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Role of secretome mesenchymal stem cells as immunomodulator in periodontal diseases

Cited by 3 publications

References 30 publications

Accelerating Wound Healing of Traumatic Ulcer with Topical Application of Dental Pulp Mesenchymal Stem Cell Secretome and Robusta Green Coffee Bean Extract Combination in vivo

Accelerating Wound Healing of Traumatic Ulcer with Topical Application of Dental Pulp Mesenchymal Stem Cell Secretome and Robusta Green Coffee Bean Extract Combination in vivo

In vivo immunomodulatory effect and safety of MSC-derived secretome

Characteristic Differences between Freeze-dried Tenogenic Secretomes and Freeze-dried Crude Secretomes of Mesenchymal Stem Cells under Hypoxic Conditions

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