operators. We note, however, that our ACE-L IOP data are comparable with Tonolab IOP measurement in unanaesthetized and unsedated C57BL/6J male mice (range 13.3 ± 0.3). 3 In summary, acepromazine provided enough sedation to allow relatively easy steady-state mouse IOP measurement over at least 1 h without concern of significant druginduced IOP variation unlike KAX-anaesthetized mice. This finding under acepromazine was replicated using two dosages having different sedation levels, with steady-state IOP measurable over a long period between 30 and 105 min after acepromazine administration. It should be noted, however, that greater sedation was associated with lower IOP. These findings are important to note when choosing anaesthesia/sedation and measurement time points to capture live mouse IOP.