Role of Streptococcus uberis adhesion molecule in the pathogenesis of Streptococcus uberis mastitis

Almeida, Ricardo Augusto Monteiro de Barros; Dego, Oudessa Kerro; Headrick, Susan I.; Lewis, Marion; Oliver, S. P.

doi:10.1016/j.vetmic.2015.07.005

Cited by 24 publications

(24 citation statements)

References 20 publications

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“…Adhesion and internalization of S. uberis in mammary epithelial cells are important early events in the establishment of mastitis in cows [ 45 ]. Adhesion of S. agalactiae to host cells is a relevant stage of colonization [ 46 , 47 ].…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells

Barros

Perciano²,

Santos

et al. 2017

Molecules

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Mastitis is an inflammation of mammary gland parenchyma that adversely affects bovine health and dairy production worldwide despite significant efforts to eradicate it. The aim of this work was to characterize the antimicrobial activity of 7-epiclusianone (7-epi), a compound extracted from the Rheedia brasiliensis fruit, its complex with copper against Streptococcus spp. isolated from bovine mastitis, and to assess their cytotoxicity to bovine mammary alveolar cells (MAC-T). The complex 7-epiclusianone-Cu (7-epi-Cu) was an amorphous green solid with optical activity. Its vibrational spectrum in the infrared region showed absorption bands in the high-frequency region, as well as bands that can be attributed to the unconjugated and conjugated stretching of the free ligand. The complex was anhydrous. One of the tested bacterial strains was not sensitive to the compounds, while the other three had MIC values of 7.8 µg mL−1 and minimum bactericidal concentration (MBC) values between 15.6 and 31.3 µg mL−1. These two compounds are bacteriostatic, did not cause damage to the cell wall and, at sub-inhibitory concentrations, did not induce bacterial adhesion. The compounds were not cytotoxic. Based on these results, 7-epi and 7-epi-Cu exhibited desirable antimicrobial properties and could potentially be used in bovine mastitis treatment.

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Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells

Barros

Perciano²,

Santos

et al. 2017

Molecules

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“…uberis expresses several surface associated proteins such as S. uberis adhesion molecule (SUAM) and extracellular matrix binding proteins, which allow it to adhere to and internalize into mammary epithelial cells, successfully inducing IMI [144][145][146]. The S. uberis adhesion molecule (SUAM) plays a central role in the adherence of S. uberis to mammary epithelial cells [147][148][149][150]. Vaccination of dairy cows with SUAM induced strong immune resposes in vaccinated cows [151].…”

Section: Vaccine Trials Against Streptococcus Uberismentioning

confidence: 99%

“…In vivo infusion of mammary quarters of dairy cows with S. uberis pre-incubated with immuneserum from SUAM vaccinated cows reduced clinical severity [152]. The SUAM gene deletion mutant strain is less pathogenic to mammary epithelial cells [153] and to dairy cows [147]. Controlled experimental efficacy studies using SUAM as vaccine antigen to control S. uberis mastitis showed that SUAM is immunogenic but the induced immunity was not protective.…”

Section: Vaccine Trials Against Streptococcus Uberismentioning

confidence: 99%

Current Status of Antimicrobial Resistance and Prospect for New Vaccines against Major Bacterial Bovine Mastitis Pathogens

Dego¹

2021

Animal Reproduction in Veterinary Medicine

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Economic losses due to bovine mastitis is estimated to be $2 billion in the United States alone. Antimicrobials are used extensively in dairy farms for prevention and treatment of mastitis and other diseases of dairy cattle. The use of antimicrobials for treatment and prevention of diseases of dairy cattle needs to be prudent to slow down the development, persistence, and spread of antimicrobial-resistant bacteria from dairy farms to humans, animals, and farm environments. Because of public health and food safety concerns regarding antimicrobial resistance and antimicrobial residues in meat and milk, alternative approaches for disease control are required. These include vaccines, improvements in housing, management practices that reduce the likelihood and effect of infectious diseases, management systems and feed formulation, studies to gain a better understanding of animal behavior, and the development of more probiotics and competitive exclusion products. Monitoring antimicrobial resistance patterns of bacterial isolates from cases of mastitis and dairy farm environments is important for treatment decisions and proper design of antimicrobial-resistance mitigation measures. It also helps to determine emergence, persistence, and potential risk of the spread of antimicrobial-resistant bacteria and resistome from these reservoirs in dairy farms to humans, animals, and farm environments.

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“…In particular, S. uberis adhesion molecule (SUAM) uses lactoferrin as a cross-bridge with host interlectin-1 to promote adherence to and internalization of S. uberis into bovine mammary epithelial cells (Fang et al, 2000;Moshynskyy et al, 2003;Almeida et al, 2006;Patel et al, 2009;Chen et al, 2011). A mutant S. uberis strain deficient in SUAM expression was attenuated for virulence in an experimentally infected mammary gland, suggesting a key role for SUAM in the pathogenesis and niche-specificity of S. uberis (Patel et al, 2009;Almeida et al, 2015).…”

Section: Streptococcus Uberis Adhesion Moleculementioning

confidence: 99%

The Role of Gram-Positive Surface Proteins in Bacterial Niche- and Host-Specialization

Pickering

Fitzgerald

2020

Front. Microbiol.

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Gram-positive bacterial pathogens have an array of proteins on their cell surface that mediate interactions with the host environment. In particular, bacterial cell wallassociated (CWA) proteins play key roles in both colonization and pathogenesis. Furthermore, some CWA proteins promote specialization for host-species or mediate colonization of specific anatomical niches within a host. In this mini review, we provide examples of the many ways by which major pathogens, such as Staphylococci, Streptococci and Listeria monocytogenes, utilize CWA proteins for both host-and niche-specialization. We describe different biological mechanisms mediated by CWA proteins including: the acquisition of iron from hemoglobin in the bloodstream, adherence to and invasion of host cells, and innate immune evasion through binding to the plasma proteins fibrinogen, immunoglobulin G, and complement. We also discuss the limitations of using animal models for understanding the role of specific CWA proteins in host-specialization and how transformative technologies, such as CRISPR-Cas, offer tremendous potential for developing transgenic models that simulate the host environment of interest. Improved understanding of the role of CWA proteins in nicheor host-specificity will allow the design of new therapeutic approaches which target key host-pathogen interactions underpinning Gram-positive bacterial infections.

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Role of Streptococcus uberis adhesion molecule in the pathogenesis of Streptococcus uberis mastitis

Cited by 24 publications

References 20 publications

Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells

Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells

Current Status of Antimicrobial Resistance and Prospect for New Vaccines against Major Bacterial Bovine Mastitis Pathogens

The Role of Gram-Positive Surface Proteins in Bacterial Niche- and Host-Specialization

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