Role of SulP, a nuclear-encoded chloroplast sulfate permease, in sulfate transport and H2evolution in Chlamydomonas reinhardtii

Chen, Hsu-Ching; Newton, A. Jamila; Melis, Anastasios

doi:10.1007/s11120-004-7157-y

Cited by 81 publications

(37 citation statements)

References 25 publications

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“…Four genes code for chloroplast envelopetargeted TM proteins (SulP and SulP2), a chloroplast stroma-targeted ATP-binding protein (Sabc), and a substrate (sulfate)-binding protein (Sbp) that is localized on the cytosolic side of the chloroplast envelope (309). Antisense mutagenesis of the SulP gene demonstrated a role in chloroplast sulfate uptake (67). FIG.…”

Section: Comparative Genomics Of Abc Systemsmentioning

confidence: 99%

Structure, Function, and Evolution of Bacterial ATP-Binding Cassette Systems

Davidson

Dassa

Orelle

et al. 2008

Microbiol Mol Biol Rev

1,187

1,137

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SUMMARY ATP-binding cassette (ABC) systems are universally distributed among living organisms and function in many different aspects of bacterial physiology. ABC transporters are best known for their role in the import of essential nutrients and the export of toxic molecules, but they can also mediate the transport of many other physiological substrates. In a classical transport reaction, two highly conserved ATP-binding domains or subunits couple the binding/hydrolysis of ATP to the translocation of particular substrates across the membrane, through interactions with membrane-spanning domains of the transporter. Variations on this basic theme involve soluble ABC ATP-binding proteins that couple ATP hydrolysis to nontransport processes, such as DNA repair and gene expression regulation. Insights into the structure, function, and mechanism of action of bacterial ABC proteins are reported, based on phylogenetic comparisons as well as classic biochemical and genetic approaches. The availability of an increasing number of high-resolution structures has provided a valuable framework for interpretation of recent studies, and realistic models have been proposed to explain how these fascinating molecular machines use complex dynamic processes to fulfill their numerous biological functions. These advances are also important for elucidating the mechanism of action of eukaryotic ABC proteins, because functional defects in many of them are responsible for severe human inherited diseases.

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Section: Comparative Genomics Of Abc Systemsmentioning

confidence: 99%

Structure, Function, and Evolution of Bacterial ATP-Binding Cassette Systems

Davidson

Dassa

Orelle

et al. 2008

Microbiol Mol Biol Rev

1,187

1,137

View full text Add to dashboard Cite

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“…The link between SNRK2.1 kinase and repression of genes regulated by anoxic conditions is intriguing but could reflect an indirect impact of S deprivation on the physiological state of the cells. For example, a decrease in photosynthetic activity and an increase in HYD transcript accumulation and H 2 production was observed in Chlamydomonas transformants harboring antisense constructs of the chloroplast SO 4 22 permease (SULP1) grown in nutrient-replete medium (Chen et al, 2005). The authors suggested that the reduced permease activity in the transformants limited the supply of S to Phosphorous deprivation (ÀP) was used as internal control.…”

Section: Putrescine Biosynthesismentioning

confidence: 99%

RNA-Seq Analysis of Sulfur-Deprived Chlamydomonas Cells Reveals Aspects of Acclimation Critical for Cell Survival

et al. 2010

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The Chlamydomonas reinhardtii transcriptome was characterized from nutrient-replete and sulfur-depleted wild-type and snrk2.1 mutant cells. This mutant is null for the regulatory Ser-Thr kinase SNRK2.1, which is required for acclimation of the alga to sulfur deprivation. The transcriptome analyses used microarray hybridization and RNA-seq technology. Quantitative RT-PCR evaluation of the results obtained by these techniques showed that RNA-seq reports a larger dynamic range of expression levels than do microarray hybridizations. Transcripts responsive to sulfur deprivation included those encoding proteins involved in sulfur acquisition and assimilation, synthesis of sulfur-containing metabolites, Cys degradation, and sulfur recycling. Furthermore, we noted potential modifications of cellular structures during sulfur deprivation, including the cell wall and complexes associated with the photosynthetic apparatus. Moreover, the data suggest that sulfur-deprived cells accumulate proteins with fewer sulfur-containing amino acids. Most of the sulfur deprivation responses are controlled by the SNRK2.1 protein kinase. The snrk2.1 mutant exhibits a set of unique responses during both sulfur-replete and sulfurdepleted conditions that are not observed in wild-type cells; the inability of this mutant to acclimate to S deprivation probably leads to elevated levels of singlet oxygen and severe oxidative stress, which ultimately causes cell death. The transcriptome results for wild-type and mutant cells strongly suggest the occurrence of massive changes in cellular physiology and metabolism as cells become depleted for sulfur and reveal aspects of acclimation that are likely critical for cell survival.

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“…In flooded crops, especially rice, the positive effect on production attributed to the presence of the freshwater fern Azolla and the cyanobacteria Anabaena azollae that develop a symbiotic relationship is well known. The annual fixation capacity of atmospheric nitrogen of this system is 350 -670 kg N fixed / ha, about 12 to 20 times higher than the fixing capacity presented by A. azollae isolated [89,90]. Wuang et al (2016) used the biomass of Spirulina platensis as an agricultural biofertilizer in different types of crops.…”

Section: Fertilizersmentioning

confidence: 88%

Microalgae biorefineries: applications and emerging technologies

Giraldo

Romo-Buchelly

Arbeláez-Pérez

et al. 2018

DYNA

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Microalgae transform CO2 into a broad portfolio of biomolecules, and thus should be considered as a valuable biotechnological platform. Despite several research programs and global efforts to establish a sustainable microalgae-based industry, most applications have not transcended academic boundaries. This limitation raises from the high transformation costs when target products are biofuels or fertilizers. Microalgae biorefinery emerges as an alternative to improve economic competitiveness, as Under such a model scope, the process inputs come from industrial waste, while biomass exploitation prioritizes the highest value molecules followed by extraction of less valuable compounds. This review describes a wide range of microalgae exploitation schemes focused on new uses of its constituents, while discussing emerging technologies designed to improve production and efficiencies as well.Keywords: microalgae; biorefinery; biofuels; fertilizers; active biomolecules. Biorefinerías de microalgas: aplicaciones actuales y nuevas tecnologías en desarrolloResumen Las microalgas transforman el CO2 en un amplio portafolio de biomoléculas, por lo cual, son consideradas una valiosa plataforma biotecnológica. A pesar de múltiples programas de investigación y esfuerzos globales para establecer una industria sostenible basada en microalgas, la mayoría de las aplicaciones potenciales no han trascendido las fronteras académicas. Esta limitación se debe a los altos costos en la transformación del producto principalmente cuando se obtiene compuestos económicos como biocombustibles y fertilizantes. La biorefinería de microalgas surge como alternativa para incrementar la competitividad económica. En este modelo, los insumos del proceso provienen de residuos industriales, mientras que la explotación de la biomasa inicia con las moléculas de alto valor y finaliza con los compuestos menos valiosos. En esta revisión se describe un amplio abanico de esquemas de explotación de microalgas enfocado en nuevos usos de sus constituyentes. Además, se exploran las tecnologías emergentes destinadas a aprovechar esta biomasa de una manera más versátil y eficiente.

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Role of SulP, a nuclear-encoded chloroplast sulfate permease, in sulfate transport and H2evolution in Chlamydomonas reinhardtii

Cited by 81 publications

References 25 publications

Structure, Function, and Evolution of Bacterial ATP-Binding Cassette Systems

Structure, Function, and Evolution of Bacterial ATP-Binding Cassette Systems

RNA-Seq Analysis of Sulfur-Deprived Chlamydomonas Cells Reveals Aspects of Acclimation Critical for Cell Survival

Microalgae biorefineries: applications and emerging technologies

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