Role of survivin expression in predicting biochemical recurrence after radical prostatectomy: a multi‐institutional study

Mathiéu, Romain; Lucca, Ilaria; Vartolomei, Mihai Dorin; Mbeutcha, Aurélie; Klatte, Tobias; Seitz, Christian; Karakiewicz, Pierre I.; Fajković, Harun; Sun, Maxine; Lotan, Yair; Montorsi, Francesco; Briganti, Alberto; Rouprêt, Morgan; Margulis, Vitaly; Rink, Michael; Rieken, Malte; Kenner, Lukas; Susani, Martin; Loidl, Wolgang; Shariat, Shahrokh F.

doi:10.1111/bju.13472

Cited by 16 publications

(11 citation statements)

References 29 publications

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“…Descriptive and functional data on the presence and role of survivin in prostate cancer published within the last years revealed highly contradictory results [13][14][15][16][17]19,[22][23][24] .…”

Section: Discussionmentioning

confidence: 99%

Subcellular Compartmentalization of Survivin is Associated with Biological Aggressiveness and Prognosis in Prostate Cancer

Hennigs

Minner

Tennstedt

et al. 2020

Sci Rep

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the role of subcellular survivin compartmentalization in the biology and prognosis of prostate cancer is unclear. We therefore investigated subcellular localization of survivin in more than 3000 prostate cancer patients by quantitative immunohistochemistry and performed transcriptomics of 250 prostate cancer patients and healthy donors using publicly available datasets. Survivin (BIRC5) gene expression was increased in primary prostate cancers and metastases, but did not differ in recurrent vs non-recurrent prostate cancers. Survivin immunohistochemistry (IHC) staining was limited exclusively to the nucleus in 900 prostate cancers (40.0%), and accompanied by various levels of cytoplasmic positivity in 1338 tumors (59.4%). 0.5% of prostate cancers did not express survivin. Nuclear and cytoplasmic survivin staining intensities were strongly associated with each other, pT category, and higher Gleason scores. cytoplasmic but not nuclear survivin staining correlated with high tumor cell proliferation in prostate cancers. Strong cytoplasmic survivin staining, but not nuclear staining predicted an unfavorable outcome in univariate analyses. Multivariate Cox regression analysis showed that survivin is not an independent prognostic marker. In conclusion, we provide evidence that survivin expression is increased in prostate cancers, especially in metastatic disease, resulting in higher aggressiveness and tumor progression. In addition, subcellular compartmentalization is an important aspect of survivin cancer biology, as only cytoplasmic, but not nuclear survivin accumulation is linked to biological aggressiveness and prognosis of prostate cancers.

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Section: Discussionmentioning

confidence: 99%

Subcellular Compartmentalization of Survivin is Associated with Biological Aggressiveness and Prognosis in Prostate Cancer

Hennigs

Minner

Tennstedt

et al. 2020

Sci Rep

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show abstract

“…Further analysis of fascin levels is needed in order to establish the correlation between elevated fascin serum levels and biopsy proven PCa or in associations with other tissue and serum markers [ 15 , 16 ]. Furthermore, the sensitivity and the specificity of the determination can be evaluated.…”

Section: Discussionmentioning

confidence: 99%

Fascin is Secreted in Male’s Serum: Results of a Pilot Study

et al. 2018

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Aim:Fascin is a 55 kDa globular protein with an important role in cell migration. Aim of study was to investigate serum fascin in healthy males.Materials & methods:From 1 July 2016 to 31 December 2016, we collected serum from 46 males. Serum fascin level was performed using ELISA kit from USBiological (Salem, MA, USA).Results:Median age was 64 years. Mean fascin serum level was 9.84 ng/ml, mean prostate-specific antigen (PSA) was 2.74 ng/ml and mean prostate volume was 37.64 cc. The 51–60 years group had a mean of 10.53 ng/ml, the 61–70 group a mean of 9.7 ng/ml and the 71–80 group had a mean of 9.41 ng/ml fascin serum level.Conclusion:Fascin serum level did not differ according to age in males.

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“…Data showing that the silencing of BIRC5 causes a decrease of cell proliferation both in vitro and in vivo have been also reported 33 , 34 . Moreover, high BIRC5 expression is related to a higher Gleason score 35 as well as with clinic-pathological features of aggressive PCa 36 , 37 .…”

Section: Discussionmentioning

confidence: 99%

Discovering the miR-26a-5p Targetome in Prostate Cancer Cells

et al. 2017

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Purpose. miR-26a-5p is a tumor suppressor (TS) miRNA often downregulated in several tumor tissues and tumor cell lines. In this work, we performed the re-expression of the miR-26a-5p in DU-145 prostate cancer cells to collect genes interacting with miR-26a-5p and analyzed their integration in the tumorigenesis related pathways.Methods. The transfection of DU-145 prostate cancer cells with miR-26a-5p was done using nucleofection. The biological effects induced by miR-26a-5p re-expression were detected with routine assays for cell proliferation, cell cycle, survival, apoptosis and cell migration. The miRNA pull out technique was used to collect and next generation sequencing to identify the complete repertoire of the miR-26a-5p targets (miR-26a-5p/targetome). TargetScan 7, PITA and RNA22 were used to find the predicted miR-26a-5p targets in the miR-26a-5p/targetome. Gene set enrichment analysis were used to integrate target genes in KEGG pathways and Protein-Protein Interaction networks (PPINs) and modules were built.Results. miR-26a-5p exerted an anti-proliferative effect acting at several levels, by decreasing survival and migration and inducing both cell cycle block and apoptosis. The analysis of the miR-26a-5p/targetome showed that 1423 (1352 coding and 71 non-coding) transcripts interacted with miR-26a-5p. Filtering the miR-26a-5p/targetome with prediction algorithms, 628 out of 1353 transcripts were miR-26a-5p predicted targets and 73 of them were already validated miR-26a-5p targets. Finally, miR-26a-5p targets were involved in 22 KEGG pathways and 20 significant protein-protein interaction modulesConclusion. The TS-miR-26a-5p/targetome is a platform that shows both unknown and known miRNA/target interactions thus offering the possibility to validate genes and discover pathways in which these genes could be involved.

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Role of survivin expression in predicting biochemical recurrence after radical prostatectomy: a multi‐institutional study

Cited by 16 publications

References 29 publications

Subcellular Compartmentalization of Survivin is Associated with Biological Aggressiveness and Prognosis in Prostate Cancer

Subcellular Compartmentalization of Survivin is Associated with Biological Aggressiveness and Prognosis in Prostate Cancer

Fascin is Secreted in Male’s Serum: Results of a Pilot Study

Discovering the miR-26a-5p Targetome in Prostate Cancer Cells

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