Role of the C Terminus of FtsK in Escherichia coli Chromosome Segregation

Yu, Xuan-Chuan; Weihe, Elizabeth; Margolin, William

doi:10.1128/.180.23.6424-6428.1998

Cited by 50 publications

(65 citation statements)

References 15 publications

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“…A second indication of partitioning difficulties in ripX cells is the presence of small anucleate cells adjoining larger cell units containing a chromosome located close to the interfacing septum. A comparable situation has been described for E. coli parC (19,33) and ftsK (16,38) mutants. In addition, the ripX mutant cultures contained rare nucleated minicells and examples of what appeared to be nucleoids guillotined by a cell division.…”

Section: Disruption Of Ripx Alters Cell and Nucleoid Morphologysupporting

confidence: 54%

The ripX Locus of Bacillus subtilis Encodes a Site-Specific Recombinase Involved in Proper Chromosome Partitioning

et al. 1999

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The Bacillus subtilis ripX gene encodes a protein that has 37 and 44% identity with the XerC and XerD site-specific recombinases of Escherichia coli. XerC and XerD are hypothesized to act in concert at the dif site to resolve dimeric chromosomes formed by recombination during replication. Cultures of ripX mutants contained a subpopulation of unequal-size cells held together in long chains. The chains included anucleate cells and cells with aberrantly dense or diffuse nucleoids, indicating a chromosome partitioning failure. This result is consistent with RipX having a role in the resolution of chromosome dimers inB. subtilis. Spores contain a single uninitiated chromosome, and analysis of germinated, outgrowing spores showed that the placement of FtsZ rings and septa is affected in ripXstrains by the first division after the initiation of germination. The introduction of a recA mutation into ripXstrains resulted in only slight modifications of the ripXphenotype, suggesting that chromosome dimers can form in a RecA-independent manner in B. subtilis. In addition to RipX, the CodV protein of B. subtilis shows extensive similarity to XerC and XerD. The RipX and CodV proteins were shown to bind in vitro to DNA containing the E. coli dif site. Together they functioned efficiently in vitro to catalyze site-specific cleavage of an artificial Holliday junction containing adif site. Inactivation of codV alone did not cause a discernible change in phenotype, and it is speculated that RipX can substitute for CodV in vivo.

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Section: Disruption Of Ripx Alters Cell and Nucleoid Morphologysupporting

confidence: 54%

The ripX Locus of Bacillus subtilis Encodes a Site-Specific Recombinase Involved in Proper Chromosome Partitioning

et al. 1999

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“…A mukB null mutation also cannot be combined with an ftsK allele that expresses only the amino terminus (cell division domain) of the protein in E. coli (165,166). In B. subtilis, an smc spoIIIE double-deletion mutant grows poorly and accumulates suppressor mutations (13).…”

Section: Mukb Smc and Chromosome Segregationmentioning

confidence: 99%

“…It was subsequently discovered that it was not the lack of cell division that resulted in the loss of dimer resolution but that the carboxyl terminus of the cell division protein FtsK was required for resolution of dimers (120,135). FtsK is a member of the SpoIIIE family of DNA translocases that localize to the septum during cell division (8,149,159,161,166). The aminoterminal domain of FtsK contains a membrane-spanning region that is essential for cell division (8,30,149,166), and the carboxyl terminus contains an ATPbinding site and is required for efficient chromosome partitioning (8,86,165).…”

Section: Integration Of Chromosome Partitioning With the Cell Cycle Amentioning

confidence: 99%

Bacterial Chromosome Segregation

Draper

Gober

2002

Annu. Rev. Microbiol.

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Recent studies have made great strides toward our understanding of the mechanisms of microbial chromosome segregation and partitioning. This review first describes the mechanisms that function to segregate newly replicated chromosomes, generating daughter molecules that are viable substrates for partitioning. Then experiments that address the mechanisms of bulk chromosome movement are summarized. Recent evidence indicates that a stationary DNA replication factory may be responsible for supplying the force necessary to move newly duplicated DNA toward the cell poles. Some factors contributing to the directionality of chromosome movement probably include centromere-like-binding proteins, DNA condensation proteins, and DNA translocation proteins.

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“…Mutations in B. subtilis smc and E. coli mukB cause strikingly similar phenotypes (1,20,21). Interestingly, it was recently reported that an E. coli mukB and ftsK double mutant, which has a postseptational partitioning function, could not be isolated (31). This further suggests that SMC and MukB play similar roles in nucleoid structure and chromosome partition- RB68 (6) Pspac-smc spoIIIE ϩ 10.6 (34/320) RB69…”

Section: Depletion Of Smc From Cells Containing Spoiiie Mutationsmentioning

confidence: 99%

Synthetic Lethal Phenotypes Caused by Mutations Affecting Chromosome Partitioning in Bacillus subtilis

Britton

Grossman

1999

J Bacteriol

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We investigated the genetic interactions between mutations affecting chromosome structure and partitioning in Bacillus subtilis. Loss-of-function mutations in spoIIIE(encoding a putative DNA translocase) and smc (involved in chromosome structure and partitioning) caused a synthetic lethal phenotype. We constructed a conditional mutation in smc and found that many of the spoIIIE smc double-mutant cells had a chromosome bisected by a division septum. The growth defect of the double mutant was exacerbated by a null mutation in the chromosome partitioning gene spo0J. These results suggest that mutants defective in nucleoid structure are unable to move chromosomes out of the way of the invaginating septum and that SpoIIIE is involved in repositioning these bisected chromosomes during vegetative growth.

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Role of the C Terminus of FtsK in Escherichia coli Chromosome Segregation

Cited by 50 publications

References 15 publications

The ripX Locus of Bacillus subtilis Encodes a Site-Specific Recombinase Involved in Proper Chromosome Partitioning

The ripX Locus of Bacillus subtilis Encodes a Site-Specific Recombinase Involved in Proper Chromosome Partitioning

Bacterial Chromosome Segregation

Synthetic Lethal Phenotypes Caused by Mutations Affecting Chromosome Partitioning in Bacillus subtilis

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