2009
DOI: 10.1128/jb.01626-08
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Role of the Extracytoplasmic Function Sigma Factor RpoE4 in Oxidative and Osmotic Stress Responses in Rhizobium etli

Abstract: The aims of this study were to functionally characterize and analyze the transcriptional regulation and transcriptome of the Rhizobium etli rpoE4 gene. An R. etli rpoE4 mutant was sensitive to oxidative, saline, and osmotic stresses. Using transcriptional fusions, we determined that RpoE4 controls its own transcription and that it is negatively regulated by rseF (regulator of sigma rpoE4; CH03274), which is cotranscribed with rpoE4. rpoE4 expression was induced not only after oxidative, saline, and osmotic sho… Show more

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Cited by 50 publications
(64 citation statements)
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“…S. meliloti rpoE2 mutants were recently described as more sensitive than the wild-type strain to desiccation (23) as well as to high H 2 O 2 concentrations in stationary phase (10), confirming the involvement of RpoE2 in stress responses. Interestingly, this sigma factor is largely conserved among alphaproteobacteria (33), and RpoE2 orthologues have been involved in stress resistance and/or host colonization in several species, including Brucella melitensis (7), Caulobacter crescentus (1), and more recently Rhizobium etli (28) and Bradyrhizobium japonicum (17). RpoE2-like sigma factors can therefore be viewed as the long-searched regulators of the general stress response in alphaproteobacteria (33).…”
mentioning
confidence: 99%
“…S. meliloti rpoE2 mutants were recently described as more sensitive than the wild-type strain to desiccation (23) as well as to high H 2 O 2 concentrations in stationary phase (10), confirming the involvement of RpoE2 in stress responses. Interestingly, this sigma factor is largely conserved among alphaproteobacteria (33), and RpoE2 orthologues have been involved in stress resistance and/or host colonization in several species, including Brucella melitensis (7), Caulobacter crescentus (1), and more recently Rhizobium etli (28) and Bradyrhizobium japonicum (17). RpoE2-like sigma factors can therefore be viewed as the long-searched regulators of the general stress response in alphaproteobacteria (33).…”
mentioning
confidence: 99%
“…In Alphaproteobacteria, the GSR is controlled by an alternative extracytoplasmic function (ECF) sigma factor, usually called σ EcfG (1) or ECF15 sigma factor (2), the activity of which is regulated by a conserved partner-switching mechanism (1,(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). In unstressed conditions, σ EcfG is sequestered by its anti-sigma factor NepR.…”
mentioning
confidence: 99%
“…This model is supported by the work recently done on Bradyrhizobium japonicum USDA110, S. meliloti and C. crescentus systems (69)(70)(71) (Figure 4). The first alphaproteobacterial PhyR was identified in Methylobacterium extorquens, and PhyR homologs were subsequently characterized also in rhizobial species and C. crescentus (70,(72)(73)(74)(75)(76)(77). EcfG sigma factors, NepR and PhyR are conserved in all free-living alpha-proteobacteria, as the only species that do not possess this system are obligate symbionts (78,79).…”
Section: Phyr An Anti-anti-sigma Factormentioning
confidence: 99%