Role of the Laboratory in Diagnosis of Influenza during Seasonal Epidemics and Potential Pandemics

Petric, Martin; Comanor, Lorraine; Petti, Cathy A.

doi:10.1086/507554

Cited by 149 publications

(159 citation statements)

References 38 publications

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“…Our results are consistent with other reports suggesting lower sensitivity among adults (16,18,22). Stein et al conducted a study among 258 adults presenting to the emergency room with a new illness associated with cough, sinus pain, nasal congestion, rhinorrhea, sore throat, or fever.…”

Section: Vol 47 2009 Sensitivity Of a Point-of-care Test For Influesupporting

confidence: 93%

Field Performance of a Rapid Diagnostic Test for Influenza in an Ambulatory Setting

et al. 2009

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Provided test characteristics are adequate, point-of-care rapid antigen detection tests for influenza could improve the timeliness and appropriateness of clinical decisions. Our objective was to estimate the field sensitivity and specificity of the Quidel QuickVue Influenza A؉B test in an ambulatory setting. The sensitivity and specificity of the Quidel QuickVue test was evaluated against reverse-transcriptase PCR (RT-PCR) on nasopharyngeal specimens collected over two consecutive influenza seasons from ambulatory patients consulting for influenza-like illness (

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Section: Vol 47 2009 Sensitivity Of a Point-of-care Test For Influesupporting

confidence: 93%

Field Performance of a Rapid Diagnostic Test for Influenza in an Ambulatory Setting

et al. 2009

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“…Compared with clinical samples from laboratory animals, samples derived from the field could be complicated with high background in qRT-PCR due to low quantities of virus analyte, inappropriately collected/handled/transported specimens, the presence of viral inhibitor, and/or the presence of proteins from other viruses or bacterial pathogens (26). Nevertheless, for vaccine strain selection in the clinical setting, it is critical to use assays with 100% specificity and relatively high sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Detection of Antigenic Variants of Subtype H3 Swine Influenza A Viruses from Clinical Samples

Martin

Bowman

et al. 2017

J Clin Microbiol

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A large population of genetically and antigenically diverse influenza A viruses (IAVs) are circulating among the swine population, playing an important role in influenza ecology. Swine IAVs not only cause outbreaks among swine but also can be transmitted to humans, causing sporadic infections and even pandemic outbreaks. Antigenic characterizations of swine IAVs are key to understanding the natural history of these viruses in swine and to selecting strains for effective vaccines. However, influenza outbreaks generally spread rapidly among swine, and the conventional methods for antigenic characterization require virus propagation, a timeconsuming process that can significantly reduce the effectiveness of vaccination programs. We developed and validated a rapid, sensitive, and robust method, the polyclonal serum-based proximity ligation assay (polyPLA), to identify antigenic variants of subtype H3N2 swine IAVs. This method utilizes oligonucleotide-conjugated polyclonal antibodies and quantifies antibody-antigen binding affinities by quantitative reverse transcription-PCR (RT-PCR). Results showed the assay can rapidly detect H3N2 IAVs directly from nasal wash or nasal swab samples collected from laboratorychallenged animals or during influenza surveillance at county fairs. In addition, polyPLA can accurately separate the viruses at two contemporary swine IAV antigenic clusters (H3N2 swine IAV-␣ and H3N2 swine IAV-ß) with a sensitivity of 84.9% and a specificity of 100.0%. The polyPLA can be routinely used in surveillance programs to detect antigenic variants of influenza viruses and to select vaccine strains for use in controlling and preventing disease in swine.

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“…In children and patients with chronic disease, the attack rate may reach up to 40% during outbreaks (3). The agents that lead to morbidity in humans are mostly influenza A and B (4). However, influenza A is mostly responsible for seasonal epidemics and worldwide pandemics.…”

Section: Introductionmentioning

confidence: 99%

Clinical differences of influenza subspecies among hospitalized children

Acar¹,

Sütçü²,

Aktürk³

et al. 2017

Turk Pediatri Ars

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Aim: Clinical findings, mortality, and morbidity rates differ among influenza subspecies. Awareness of these differences will lead physicians to choose the proper diagnostic and therapeutic strategies and to foresee possible complications. The aim of this study was to evaluate the clinical differences of influenza subspecies among hospitalized children. Material and Methods:Hospitalized children with proven influenza infection by polymerase chain reaction on nasopharyngeal swab specimens in our clinic, between December 2013 and March 2016, were enrolled. These children were divided into 3 groups as Influenza A/H1N1 (n=42), Influenza A/H3N2 (n=23), and Influenza B (n=35). Results:The median age of the children was 51.5 months (range, 3-204 months). The most common presenting symptoms were fever (n=83), cough (n=58), and difficulty in breathing (n=25). The most common non-respiratory findings were lymphadenopathy (n=18) and gastrointestinal system involvement (n=17). Sixty-two percent of the patients (n=62) had chronic diseases. H1N1 and H3N2 were significantly more common among patients with chronic neurologic disorders and renal failure, respectively. Leukopenia (n=32) and thrombocytopenia (n=22) were the most common pathologic laboratory findings. Neutropenia, elevated CRP levels, and antibiotic use were significantly more common among patients with H1N1 infection. Seven patients were transferred to the intensive care unit with diagnoses of acute respiratory distress syndrome (n=4), encephalitis (n=2), and bronchiolitis (n=1). Two patients with chronic diseases and H1N1 infection died secondary to acute respiratory distress syndrome.Conclusions: Influenza A/H1N1 infection represented more severe clinical disease. (Turk Pediatri Ars 2017; 52: 15-22)

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Role of the Laboratory in Diagnosis of Influenza during Seasonal Epidemics and Potential Pandemics

Cited by 149 publications

References 38 publications

Field Performance of a Rapid Diagnostic Test for Influenza in an Ambulatory Setting

Field Performance of a Rapid Diagnostic Test for Influenza in an Ambulatory Setting

Detection of Antigenic Variants of Subtype H3 Swine Influenza A Viruses from Clinical Samples

Clinical differences of influenza subspecies among hospitalized children

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