Role of the Methionine Sulfoxide Reductase MsrB3 in Cold Acclimation in Arabidopsis

Ethylene is a key signal in the regulation of plant defense responses. It is required for the expression and function of GDSL LIPASE1 (GLIP1) in Arabidopsis (Arabidopsis thaliana), which plays an important role in plant immunity. Here, we explore molecular mechanisms underlying the relationship between GLIP1 and ethylene signaling by an epistatic analysis of ethylene response mutants and GLIP1-overexpressing (35S:GLIP1) plants. We show that GLIP1 expression is regulated by ethylene signaling components and, further, that GLIP1 expression or application of petiole exudates from 35S:GLIP1 plants affects ethylene signaling both positively and negatively, leading to ETHYLENE RESPONSE FACTOR1 activation and ETHYLENE INSENSITIVE3 (EIN3) down-regulation, respectively. Additionally, 35S:GLIP1 plants or their exudates increase the expression of the salicylic acid biosynthesis gene SALICYLIC ACID INDUCTION-DEFICIENT2, known to be inhibited by EIN3 and EIN3-LIKE1. These results suggest that GLIP1 regulates plant immunity through positive and negative feedback regulation of ethylene signaling, and this is mediated by its activity to accumulate a systemic signal(s) in the phloem. We propose a model explaining how GLIP1 regulates the fine-tuning of ethylene signaling and ethylene-salicylic acid cross talk.

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“…To measure chlorophyll content, leaves were submerged in 95% ethanol and incubated for 20 min at 80°C (Kwon et al, 2007 (Lichtenthaler, 1987).…”

Section: Determination Of Chlorophyll Contentmentioning

confidence: 99%

GDSL LIPASE1 Modulates Plant Immunity through Feedback Regulation of Ethylene Signaling

Kim

Kwon²,

Jang³

et al. 2013

Plant Physiology

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“…There is ample evidence that ROS are also crucial second messengers involved in the response to diverse abiotic and biotic forms of stress. An oxidative burst takes place in response to avirulent microbial intruders (Lamb and Dixon, 1997) or to the previously mentioned abiotic stress factors including heat (Wahid et al, 2007), cold (Kwon et al, 2007), drought, salinity (Miller et al, 2010) and others. ROS production in plants by plasma membrane NADPH oxidases and apoplastic oxidases following pathogen recognition is well documented process (Allan and Fluhr, 1997;Lamb and Dixon, 1997;Bolwell et al, 2002;Torres et al, 2006;Galletti et al, 2008).…”

Section: +mentioning

confidence: 99%

Abiotic and Biotic Stress Response Crosstalk in Plants

Fraire-Velázquez¹,

Rodríguez-Guerra²,

Sánchez-Calderón³

2011

Abiotic Stress Response in Plants - Physiological, Biochemical and Genetic Perspectives

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“…The role of oxidation of methionine (Met) in methionine sulfoxide (MetO) and its repair by methionine sulfoxide reductases (MSRs) has not been documented in seeds, although several lines of evidence support the possible involvement of MSRs in maturation and germination processes: (i) MSRs belong to the minimal set of proteins required for basic functions in living cells (20); (ii) Met oxidation is a hallmark of oxidative conditions and aging in all organisms (21); (iii) MSRs play a determinant role in the tolerance of oxidative stress (22)(23)(24)(25)(26)(27)(28); and (iv) MSRs control lifespan in microorganisms, insects, and mammals, including human (29)(30)(31)(32). Moreover, one MSR gene exhibits increased expression during reinduction of desiccation tolerance in germinated seeds of Medicago truncatula (33).…”

mentioning

confidence: 99%

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

Châtelain

Satour

Laugier

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

102

103

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Seeds are in a natural oxidative context leading to protein oxidation. Although inevitable for proper progression from maturation to germination, protein oxidation at high levels is detrimental and associated with seed aging. Oxidation of methionine to methionine sulfoxide is a common form of damage observed during aging in all organisms. This damage is reversible through the action of methionine sulfoxide reductases (MSRs), which play key roles in lifespan control in yeast and animal cells. To investigate the relationship between MSR capacity and longevity in plant seeds, we first used two Medicago truncatula genotypes with contrasting seed quality. After characterizing the MSR family in this species, we analyzed gene expression and enzymatic activity in immature and mature seeds exhibiting distinct quality levels. We found a very strong correlation between the initial MSR capacities in different lots of mature seeds of the two genotypes and the time to a drop in viability to 50% after controlled deterioration. We then analyzed seed longevity in Arabidopsis thaliana lines, in which MSR gene expression has been genetically altered, and observed a positive correlation between MSR capacity and longevity in these seeds as well. Based on our data, we propose that the MSR repair system plays a decisive role in the establishment and preservation of longevity in plant seeds.seed deterioration | seed viability | redox systems | antioxidant enzymes S uccessful crop implantation is crucial for maximal plant yield and relies on seed physiological quality, which is acquired at the end of development, during maturation. Quality is expressed by the capacity of seeds to germinate in a fast and homogenous manner before and after storage (seed longevity) and to provide proper seedling establishment in various environments (seed vigor). Quality is controlled by genetic traits and varies greatly among species and cultivars. The establishment of quality also depends on the prevailing environmental conditions during seed development and storage (1, 2).During the maturation phase, mechanisms are put in place to prevent and repair desiccation damage, as well as to allow survival of seeds in the dry state (3-5). Nonetheless, drying of seeds on mother plants can occur prematurely in drought conditions, leading to low-quality seeds (6-8). Thus, in the context of a warming climate, producing good-quality seeds represents a global challenge to sustain current yield levels (9, 10).The mechanisms underlying seed quality are controlled in part by abscisic acid (ABA), the phytohormone involved in plant responses to dehydration that is produced during seed maturation. Of note, ABA triggers the accumulation of late-embryogenesis abundant proteins, heat-shock proteins (HSPs), and storage proteins (11). Late-embryogenesis abundant proteins and HSPs are thought to primarily protect cell structures when water is removed. Reserve proteins, through their sensitivity to reactive oxygen species (ROS), play a determinant role in limiting oxidative damage...

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Role of the Methionine Sulfoxide Reductase MsrB3 in Cold Acclimation in Arabidopsis

Cited by 84 publications

References 63 publications

GDSL LIPASE1 Modulates Plant Immunity through Feedback Regulation of Ethylene Signaling

GDSL LIPASE1 Modulates Plant Immunity through Feedback Regulation of Ethylene Signaling

Abiotic and Biotic Stress Response Crosstalk in Plants

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

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