Role of the p38 MAP kinase pathway in <em>C. elegans</em> surface antigen switching

Politz, Samuel M.

doi:10.17912/micropub.biology.000130

Cited by 1 publication

(1 citation statement)

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“…Surprisingly, srf-6 has recently been found to correspond to nsy-1 , a neuronal gene encoding a p38 MAP kinase pathway component ( Van Sciver et al . 2019 ), which suggests that neuronal signal transduction pathways can modulate the expression of surface antigens ( Politz 2019 ). Similarly, chemotaxis mutants such as tax-4 exhibit altered surface properties ( Yook and Hodgkin 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Isolation and molecular identification of nematode surface mutants with resistance to bacterial pathogens

O’Rourke

Gravato-Nobre

Stroud

et al. 2023

G3: Genes, Genomes, Genetics

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Numerous mutants of the nematode Caenorhabditis elegans with surface abnormalities have been isolated by utilizing their resistance to a variety of bacterial pathogens (Microbacterium nematophilum, Yersinia pseudotuberculosis and two Leucobacter strains), all of which are able to cause disease or death when worms are grown on bacterial lawns containing these pathogens. Previous work led to the identification of nine srf or bus genes; here we report molecular identification and characterization of a further ten surface-affecting genes. Three of these were found to encode factors implicated in glycosylation (srf-2, bus-5, bus-22), like several of those previously reported; srf-2 belongs to the GT92 family of putative galactosyltransferases, and bus-5 is homologous to human TGDS, which is implicated in Catel-Manzke syndrome. Other genes encoded proteins with sequence similarity to phosphatidylinositol phosphatases (bus-6), Patched-related receptors (ptr-15/bus-13), steroid dehydrogenases (dhs-5/bus-21) or glypiation factors (bus-24). Three genes appeared to be nematode-specific (srf-5, bus-10, bus-28). Many mutants exhibited cuticle fragility as revealed by bleach and detergent sensitivity; this fragility was correlated with increased drug sensitivity, as well as with abnormal skiddy locomotion. Most of the genes examined were found to be expressed in epidermal seam cells, which appear to be important for synthesizing nematode surface coat. The results reveal the genetic and biochemical complexity of this critical surface layer, and provide new tools for its analysis.

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Section: Discussionmentioning

confidence: 99%