2011
DOI: 10.1371/journal.pone.0016842
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Role of the Small GTPase Rho3 in Golgi/Endosome Trafficking through Functional Interaction with Adaptin in Fission Yeast

Abstract: BackgroundWe had previously identified the mutant allele of apm1+ that encodes a homolog of the mammalian µ1A subunit of the clathrin-associated adaptor protein-1 (AP-1) complex, and we demonstrated the role of Apm1 in Golgi/endosome trafficking, secretion, and vacuole fusion in fission yeast.Methodology/Principal FindingsIn the present study, we isolated rho3+, which encodes a Rho-family small GTPase, an important regulator of exocystosis, as a multicopy-suppressor of the temperature-sensitive growth of the a… Show more

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Cited by 21 publications
(37 citation statements)
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References 27 publications
(46 reference statements)
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“…Rho3 binds to different subunits of the AP-1 complex, which mediates transport from Golgi to endosomes. Thus, Rho3 interacts with the AP-1 accessory protein Sip1 in a nucleotideindependent way, but interacts with Apm1 preferentially in its active form (Kita et al, 2011;Yu et al, 2013). Taken together, these data indicate that exocyst localization might be precisely regulated in time and space and several GTPases such as Cdc42, Rho4 and Rho3 are involved in this process.…”
Section: Rho4 and Its Relationship With The Exocystmentioning
confidence: 74%
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“…Rho3 binds to different subunits of the AP-1 complex, which mediates transport from Golgi to endosomes. Thus, Rho3 interacts with the AP-1 accessory protein Sip1 in a nucleotideindependent way, but interacts with Apm1 preferentially in its active form (Kita et al, 2011;Yu et al, 2013). Taken together, these data indicate that exocyst localization might be precisely regulated in time and space and several GTPases such as Cdc42, Rho4 and Rho3 are involved in this process.…”
Section: Rho4 and Its Relationship With The Exocystmentioning
confidence: 74%
“…6c). It is known that rho3D accumulate abnormal electrodense membranes inside the cells and have secretion defects (Kita et al, 2011), so it is possible that Exo70 is retained in these internal membranes. In summary, rho3D rho4D double mutant displayed additive effects (Fig.…”
Section: Rho3 and Rho4 Display Different Roles During Cell Separationmentioning
confidence: 99%
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“…8D). Rho3 is involved in polarized cell growth by regulating proper localization of formin For3 and the exocyst (58,59) and in the control of Golgi-endosome trafficking (60). Interestingly, increased expression of Erf2/Erf4 enhances the palmitoylation state of Rho3 to promote meiotic entry (49).…”
Section: Discussionmentioning
confidence: 99%
“…In S. pombe, mutations in exocyst components show defects in cell separation (28,31,32). Rho3p restored exocyst localization in a cdc42 thermosensitive mutant with multiple membrane traffic defects (33), and it has been implicated in the regulation of Golgi/endosome trafficking through a functional interaction with adaptin (clathrin-associated adaptor protein-1) and Sip1p (34,35). Thus, it is possible that Rho3p could stimulate secretion by locally increasing the exocytic apparatus or through Golgi/endosome regulation.…”
mentioning
confidence: 99%