Role Of Toll Like Receptors In Various Diseases

Güven, Berrak; Çan, Murat

doi:10.5505/sakaryamj.2012.47550

Cited by 3 publications

(1 citation statement)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Toll was rst identi ed as the gene encoding the transmembrane protein in the species Droshopilla melanogaster [6]. With later studies, the homolog of the protein encoded by the toll gene was detected in mammals and named as toll-like receptor (TLR) [7]. TLR4, a member of the TLR family, is known as a cell membrane receptor that activates with both LPS (Lipopolysaccarides) and DAMPs (Damage-associated molecular pattern) and causes proin ammatory cytokine synthesis.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the effects of the toll-like receptor 4 pathway on immune checkpoint vista in pancreatic cancer

et al. 2022

View full text Add to dashboard Cite

Pancreatic ductal adenocarcinoma (PDAC) is one of the most common malignant tumors of the pancreas. Preclinical studies show that it evades the immune system with immune checkpoints and promotes tumor development. V-domain Ig suppressor of T cell activation (VISTA) is a new immunecheck point from the B7 family and is highly expressed in cancer cells. Overexpression of toll like receptor 4 (TLR4) in pancreatic adenocarcinoma is associated with inducing tumorigenesis, tumor growth and resistance to chemotherapy. Naloxone is an opioid and inhibits TLR4-ligand association. In this study, we investigated the connection of TLR4 and downstream pathway with immune-check point VISTA in pancreatic cancer proliferation. We rst collected pancreatic cancer-related datasets using the GEPIA2 and UALCAN databases. Based on the data obtained the effect of different concentrations and incubation times of Naloxone were used on PANC-1 cells proliferation. Combination of Naloxone and VISTA-siRNA were applied, and effect of both Naloxone and combined reatment on TLR4, Interleukin 1 receptor associated kinase 4 (IRAK4) and VISTA gene expression were analysed in pancreatic cancer cells. As a result of analysis with Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), gene expression levels of TLR4, IRAK4 and VISTA were signi cantly suppressed and cell proliferation was signi cantly reduced. We found that administration of Naloxone and VISTA-siRNA in combination to PDAC cells suppressed signaling. Therefore, we considered that the relationship between VISTA and TLR4 signaling pathways and the other possible associated signal molecules may be an important marker in determining the response of immune checkpoint inhibitors in cancer treatment.

show abstract

Section: Introductionmentioning

confidence: 99%

Investigation of the effects of the toll-like receptor 4 pathway on immune checkpoint vista in pancreatic cancer

et al. 2022

View full text Add to dashboard Cite

show abstract

Glycoside oleandrin downregulates toll-like receptor pathway genes and associated miRNAs in human melanoma cells

Güneş

Çelik

Seçme

et al. 2022

Gene

View full text Add to dashboard Cite

Investigation of The Effects of The Toll-Like Receptor 4 Pathway on Immune Checkpoint Vista in Pancreatic Cancer

Korucu

Menevşe

Koçak

et al. 2021

Preprint

View full text Add to dashboard Cite

Pancreatic ductal adenocarcinoma (PDAC) is one of the most common malignant tumors of the pancreas. Preclinical studies show that it evades the immune system with immune checkpoints and promotes tumor development. V-domain Ig suppressor of T cell activation (VISTA) is a new immune-check point from the B7 family and is highly expressed in cancer cells. Overexpression of toll like receptor 4 (TLR4) in pancreatic adenocarcinoma is associated with inducing tumorigenesis, tumor growth and resistance to chemotherapy. Naloxone is an opioid and inhibits TLR4-ligand association. In this study, we investigated the connection of TLR4 and downstream pathway with immune-check point VISTA in pancreatic cancer proliferation. We first collected pancreatic cancer-related datasets using the GEPIA2 and UALCAN databases. Based on the data obtained the effect of different concentrations and incubation times of Naloxone were used on PANC-1 cells proliferation. Combination of Naloxone and VISTA-siRNA were applied, and effect of both Naloxone and combined reatment on TLR4, Interleukin 1 receptor associated kinase 4 (IRAK4) and VISTA gene expression were analysed in pancreatic cancer cells. As a result of analysis with Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), gene expression levels of TLR4, IRAK4 and VISTA were significantly suppressed and cell proliferation was significantly reduced. We found that administration of Naloxone and VISTA-siRNA in combination to PDAC cells suppressed signaling. Therefore, we considered that the relationship between VISTA and TLR4 signaling pathways and the other possible associated signal molecules may be an important marker in determining the response of immune checkpoint inhibitors in cancer treatment.

show abstract

Role Of Toll Like Receptors In Various Diseases

Cited by 3 publications

References 103 publications

Investigation of the effects of the toll-like receptor 4 pathway on immune checkpoint vista in pancreatic cancer

Investigation of the effects of the toll-like receptor 4 pathway on immune checkpoint vista in pancreatic cancer

Glycoside oleandrin downregulates toll-like receptor pathway genes and associated miRNAs in human melanoma cells

Investigation of The Effects of The Toll-Like Receptor 4 Pathway on Immune Checkpoint Vista in Pancreatic Cancer

Contact Info

Product

Resources

About