The ultrasonic bone scalpel is a cutting-edge surgical instrument that leverages ultrasonic mechanical effects to remove bone tissue with precision, safety, and efficacy. While the adoption of the instrument has surged, the intricate relationship between its mechanical effects and the efficiency of bone removal remains ambiguous. This study aimed to elucidate the quantitative interplay between the mechanical parameters of the instrument and its bone removal performance. To achieve this, we engineered a testing apparatus capable of consistently regulating the scalpel’s cutting force, traversal speed, and depth of cut. The bone removal proficiency of the instrument was then assessed under varying conditions: cutting forces, ultrasonic power outputs, tip traversal speeds, and bone densities. Comprehensive data analysis unveiled a distinct quantitative correlation between the aforementioned parameters and bone removal rates. Furthermore, we identified optimal parameter combinations that maximize cutting efficiency. These insights are pivotal for the design, fabrication, evaluation, and clinical application of ultrasonic bone scalpels, offering a robust reference for future advancements in the field.