Role of whole bone marrow, whole bone marrow cultured cells, and mesenchymal stem cells in chronic wound healing

Rodriguez-Menocal, Luis; Shareef, Shahjahan; Salgado, Marcela; Shabbir, Arsalan; Badiavas, Evangelos V.

doi:10.1186/s13287-015-0001-9

Cited by 92 publications

(55 citation statements)

References 26 publications

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“…However, these methods provide very limited results in most cases (up to 50%) and new therapies are required to overcome the current challenges of chronic wound management (Greer et al, 2013; Dreifke et al, 2015; Rowan et al, 2015; Sessions et al, 2017). Recent studies have indicated that cell based therapies can provide a suitable alternative treatment method for multiple pathological conditions including, wound healing (Chen et al, 2012; Rodriguez-Menocal et al, 2015; Duscher et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

TGF-β1 Pretreatment Improves the Function of Mesenchymal Stem Cells in the Wound Bed

Ghosh

McGrail

Dawson

2017

Front. Cell Dev. Biol.

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The wound healing process initiates after injury to a tissue and involves a series of orchestrated events to minimize the invasion of foreign matters such as bacteria and efficiently regenerate the damaged tissue. A variety of cells must be recruited to the tissue during wound healing. However, this process is severely disrupted in patients suffering from chronic illness, including diabetes, leading to impaired healing or non-healing wounds. Current avenues of treatment include negative-pressure therapy, wound debridement, growth factor replacement, and cell-based therapies. Among these therapies, mesenchymal stem cells (MSCs) delivery to the wound holds a very high promise due to the innate abilities of MSCs that include immunogenicity, plasticity, and self-renewal. Bone marrow derived MSCs have been shown to promote more rapid wound healing by increased cytokine production in diabetic mice. However, the lack of understanding of the mechanical and chemical interaction of the transplanted MSCs with the factors present in the regenerative niches limits their efficacy in the wound bed. In this study, we sought to understand how the changes in MSC biochemical and biophysical properties can affect their function in vitro and in vivo. We demonstrate that pretreatment of MSCs with the mechano-stimulatory soluble factor transforming growth factor (TGF-β1), which is highly expressed in injury sites, improves wound closure in a syngeneic murine wound model. This improved wound closure correlated with increased invasion into the wound bed. In vitro studies demonstrated that TGF-β1 pretreatment expedited wound closure by increasing adhesion, traction force, and migration even after removal of the stimulus. Furthermore, this response was mediated by the cytoskeletal protein focal adhesion kinase. Taken together, this study suggests that defined chemical stimuli can benefit site specific adaptability of MSCs to improve their function and therapeutic usefulness.

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Section: Introductionmentioning

confidence: 99%

TGF-β1 Pretreatment Improves the Function of Mesenchymal Stem Cells in the Wound Bed

Ghosh

McGrail

Dawson

2017

Front. Cell Dev. Biol.

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“…The dysfunctional healing of the wounds in this study can be characterized by inhibition of the inflammatory response, dysfunctional macrophages leading to an inability to combat infection, impaired angiogenesis and vasculogenesis, accumulation of fibrous tissue, and aberrant extracellular matrix accumulation. 13 There is no consensus definition of a chronic wound, but most consider a wound to be chronic if it has not healed in 4-6 weeks. 14 The recorded number of NPWT treatments patients received before dressing collection for this study ranged from 1 (one patient) to 14 (one patient).…”

Section: Resultsmentioning

confidence: 99%

Peroxy Pyruvic Acid-Containing Topical Anti-Infective: A Potential Candidate for a Wound Instillation Solution

Neas

Dunn

Silva

et al. 2016

Advances in Wound Care

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Objective: Evaluate the therapeutic properties of a peroxy pyruvic acid (PPA)-containing topical anti-infective in a human ex-vivo model that replicates the natural conditions of a human chronic wound. Approach: Wound material was extracted from patients with nonhealing diabetic ulcers, venous stasis ulcers, and arterial wounds. Microbial species were identified, and wound colonization was quantified. Extracted samples were then exposed to a PPA-containing topical anti-infective as an instillation solution with negative pressure wound therapy NPWT at concentrations of 1,000, 1,500, or 2,500 ppm for a period of 1, 5, or 10 min to determine the effect of exposure on isolated pathogens, including effect on proteins.

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“…RodriguezMenocal et al (35) demonstrated decreased motility and migration capability of MSCs in an irradiated murine delayed wound healing model. Henke et al (36) reported decreased motility and contractility in prostate CAFs, in their study associated with an increase of focal adhesion kinase.…”

Section: Discussionmentioning

confidence: 99%

Long-term changes in the properties of skin-derived fibroblasts following irradiation of the head and neck

et al. 2017

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Abstract. The tumor stroma performs an important role in carcinogenesis. It predominantly consists of fibroblasts and the connective tissue produced by them, and undergoes a multitude of interactions with the surrounding cancer cells. Since irradiation is part of the majority of therapeutic strategies for head and neck squamous cell carcinoma, more information regarding the effects of a previous irradiation on the tumor stroma is desirable. In the present study, fibroblasts were cultivated from human non-irradiated and pre-irradiated skin of the neck for 48 h. Subsequently, analyses of cell viability, apoptosis, necrosis and motility were conducted via MTT assay, Annexin V/propidium iodide staining, electronic cell counting for 4 consecutive days, and scratch assay. Pre-irradiated fibroblasts exhibited a significantly slower growth rate as well as increased rates of apoptosis and necrosis. They also exhibited significantly decreased motility compared with non-irradiated fibroblasts. These results indicated the long-term effects of irradiation on fibroblasts, which may affect cancer recurrence in the irradiated region via the tumor stroma. More information, such as that regarding the secretory capacities of pre-irradiated fibroblasts, is required to evaluate the possible therapeutic implications of these findings.

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Role of whole bone marrow, whole bone marrow cultured cells, and mesenchymal stem cells in chronic wound healing

Cited by 92 publications

References 26 publications

TGF-β1 Pretreatment Improves the Function of Mesenchymal Stem Cells in the Wound Bed

TGF-β1 Pretreatment Improves the Function of Mesenchymal Stem Cells in the Wound Bed

Peroxy Pyruvic Acid-Containing Topical Anti-Infective: A Potential Candidate for a Wound Instillation Solution

Long-term changes in the properties of skin-derived fibroblasts following irradiation of the head and neck

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