Role of Wnt5a in periodontal tissue development, maintenance, and periodontitis: Implications for periodontal regeneration (Review)

Wei, Xiuqun; Liu, Qian; Guo, Shujuan; Wu, Yafei

doi:10.3892/mmr.2020.11806

Cited by 11 publications

(12 citation statements)

References 69 publications

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“…Wnt5a has been acknowledged to participate in the pathogenesis of periodontitis, take part in the development of periodontium, promote fibrinogenesis, and moderate osteogenesis in periodontal stem cells ( Wei et al, 2021 ). In early periodontitis, Wnt5a was overexpressed in periodontal ligament tissue and regulated the CaMKII pathway, which should be involved in maintaining periodontal homeostasis ( Qian et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, combination of Wnt5a with 100 ng/ml BMP2 significantly promoted the osteogenic differentiation of dental follicle stem cells ( Xiang et al, 2014 ). These pieces of evidence suggest that Wnt5a has a bidirectional function on the osteogenic differentiation of periodontal stem cells ( Wei et al, 2021 ). We will further investigate the significance of GMSC-Exo regulation of Wnt5a in the osteogenesis and periodontal tissue regeneration of PDLSCs in future studies.…”

Section: Discussionmentioning

confidence: 99%

“…Accumulating evidence suggests that the nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB) signaling pathway and Wnt family member 5a (Wnt5a) are significant for the development and disease of periodontal tissues ( Kurgan and Kantarci, 2018 ; Wei et al, 2021 ). It has been reported that inhibition of the NF-κB signaling pathway downregulated the overexpression of Wnt5a induced by injury ( Sucre et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Exosomes Derived From Human Gingival Mesenchymal Stem Cells Attenuate the Inflammatory Response in Periodontal Ligament Stem Cells

Sun

Wang

Liu³

et al. 2022

Front. Chem.

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This study aimed to explore the effects of exosomes derived from human gingival mesenchymal stem cells (GMSC-Exo) on the inflammatory response of periodontal ligament stem cells (PDLSCs) in an inflammatory microenvironment in order to restore the regenerative potential of PDLSCs, which promotes periodontal tissue regeneration in patients with periodontitis. Periodontitis is a chronic infectious disease characterized by periodontal tissue inflammation and alveolar bone destruction. PDLSCs are regarded as promising seed cells for restoring periodontal tissue defects because of their ability to regenerate cementum/PDL-like tissue and alveolar bone. However, PDLSCs in the inflammatory environment show significantly attenuated regenerative potential. GMSC-Exo have been reported to have anti-inflammatory and immunosuppressive properties. In this study, we investigated the effects of GMSC-Exo on the inflammatory response of PDLSCs induced by lipopolysaccharides (LPS). LPS was used to simulate the inflammatory microenvironment of periodontitis in vitro. GMSC-Exo were extracted from the culture supernatant of GMSCs by ultracentrifugation. We found that GMSC-Exo attenuated the inflammatory response of PDLSCs induced by LPS. Furthermore, compared to treatment with LPS, treatment with GMSC-Exo attenuated the expression of NF-κB signaling and Wnt5a in LPS-induced PDLSCs. In conclusion, we confirmed that GMSC-Exo could suppress the inflammatory response of PDLSCs by regulating the expression of NF-κB signaling and Wnt5a, which paves the way for the establishment of a therapeutic approach for periodontitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exosomes Derived From Human Gingival Mesenchymal Stem Cells Attenuate the Inflammatory Response in Periodontal Ligament Stem Cells

Sun

Wang

Liu³

et al. 2022

Front. Chem.

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“…The role of the Wnt pathway in periodontitis has been reviewed recently (88,89). Here, we complement and advance these observations by showing that proper balance between Wnt modalities, rather than independent modulation of single pathways, might be a better approach to restore tissue balance in periodontitis.…”

Section: Discussionmentioning

confidence: 55%

Wnt Signaling in Periodontal Disease

2021

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Periodontitis is a multifactorial and chronic condition associated with the formation of a dysbiotic biofilm, leading to a pro-inflammatory environment that can modulate cell signaling. The Wnt pathway plays fundamental roles during homeostasis and disease, and emerging evidence suggests its involvement in the maintenance of the periodontium and the development of periodontitis. Here, we summarize the role of the Wnt/β-catenin and non-canonical Wnt signaling pathways in periodontitis. The accumulated data suggests specific roles for each branch of the Wnt pathway. Wnt5a emerges as a critical player promoting periodontal ligament remodeling and impairing regenerative responses modulated by the Wnt/β-catenin pathway, such as alveolar bone formation. Collectively, the evidence suggests that achieving a proper balance between the Wnt/β-catenin and non-canonical pathways, rather than their independent modulation, might contribute to controlling the progression and severity of the periodontal disease.

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“…The osteogenic property of Wnt10b has been indirectly confirmed by the observations that mice that do not express it, show a reduction of bone mass and that, in mouse embryonal development, the expression of Wnt10b induces fibroblast differentiation to osteoblasts [46]. The increased Wnt5a can also explain the increment of the inflammatory indices, since it has been reported that IL-1β, IL-6, IL-8, and TNF-α increase consequent to Wnt5a enhancement [47,48].…”

Section: Wntmentioning

confidence: 81%

Biomolecular, Histological, Clinical, and Radiological Analyses of Dental Implant Bone Sites Prepared Using Magnetic Mallet Technology: A Pilot Study in Animals

et al. 2021

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Background. A new instrumentation exploiting magneto-dynamic technology (mallet) proposed for implant site preparation was investigated. Methods. In the tibias of three minipigs, two sites were prepared by mallet and two by drill technique. Primary stability (ISQ) was detected after implant positioning (T0) and at 14 days (T14). X-rays and computed tomography were performed. At T14, bone samples were utilized for histological and biomolecular analyses. Results. In mallet sites, histological evaluations evidenced a significant increase in the newly formed bone, osteoblast number, and a smaller quantity of fibrous tissue. These results agree with the significant BMP-4 augmentation and the positive trend in other osteogenic factors (biological and radiological investigations). Major, albeit IL-10-controlled, inflammation was present. For both techniques, at T14 a significant ISQ increase was evidenced, but no significant difference was observed at T0 and T14 between the mallet and drill techniques. In mallet sites, lateral bone condensation was observed on computed tomography. Conclusions. Using biological, histological, clinical, and radiological analyses, this study first shows that the mallet technique is effective for implant site preparation. Based on its ability to cause osseocondensation and improve newly formed bone, mallet technology should be chosen in all clinical cases of poor bone quality.

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Role of Wnt5a in periodontal tissue development, maintenance, and periodontitis: Implications for periodontal regeneration (Review)

Cited by 11 publications

References 69 publications

Exosomes Derived From Human Gingival Mesenchymal Stem Cells Attenuate the Inflammatory Response in Periodontal Ligament Stem Cells

Exosomes Derived From Human Gingival Mesenchymal Stem Cells Attenuate the Inflammatory Response in Periodontal Ligament Stem Cells

Wnt Signaling in Periodontal Disease

Biomolecular, Histological, Clinical, and Radiological Analyses of Dental Implant Bone Sites Prepared Using Magnetic Mallet Technology: A Pilot Study in Animals

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