Roles of Biotin in Growing Ovarian Follicles and Embryonic Development in Domestic Fowl

Abstract

Summary It is well known that biotin deficiency causes morphological anomalies in hatchlings of fowl. An abundance of biotin in the yolk, therefore, is greatly required for maintaining reproductive functions. Although there is growing evidence for the molecular significance of the vitamin in the processes of the reproductive system in mammals, little is known about its use and roles in fowl's yolk. This review focuses on studies on the roles of biotin in the ovarian follicles and embryos of domestic fowl. Recent studies from our laboratory showed an analysis of the mechanism of biotin supply from the maternal body to follicles, or the yolk using the eggs of domestic fowl. In growing ovarian follicles, biotin was incorporated into the follicles, particularly in a large amount immediately before ovulation. Biotin incorporated from the early stage to the growth stage of ovarian follicles was stored in the protein-binding form and became free biotin again immediately before ovulation. This may be because the biotin necessary for the maintenance of embryonic growth and development should be used immediately after fertilization. In embryos, free biotin in the yolk increased shortly after fertilization. A large amount of free biotin was incorporated into the embryo at the age of 3-4 d. Biotin supply to the embryo differed among embryonic growth stages and organs, suggesting involvement in the formation of each tissue and organ. Thus, a large amount of biotin was utilized for embryonic growth, suggesting its important role in normal embryonic growth. These findings show that biotin is an essential nutrient and may play a major role in the normal morphogenesis of embryos in domestic fowl.

Effect of Housing System and Dietary Biotin Supplementation on Productive Performance, Some Blood Constituents and Economic Efficiency of Benha Line Chicken

“…However, pullets of control one was delayed in reaching to age of sexual maturity (154.05 day). The results were agreed with those reported by Taniguchi and Watanabe (2007) they demonstrated that the age at sexual maturity significant affected with biotin supplementation levels scenes it promote the early growing ovarian follicles, incorporated into the follicles, particularly in a large amount immediately before ovulation. It incorporated from the early stage to the growth stage of ovarian follicles that was stored in the protein-binding form and became available again immediately before the ovulation.…”

Transplacental transport and tissue distribution of biotin in mice at midgestation

“…From these findings, it is suggested that biotin may be associated with the expression and production of these carboxylases and organic acids may be concerned with retardation of palatal formation in vivo. Also, the presence of ACC was only slight, even in biotin-supplemented embryos as well as in the fertilized eggs of hens (Taniguchi & Watanabe 2007). Thus, fatty acid synthesis may not work in the embryos of fowl or mammals.…”

Pre-hybridisation: An efficient way of suppressing endogenous biotin-binding activity inherent to biotin–streptavidin detection system

“…The method allows application of the biotinstreptavidin detection system to perform Western blot work with cells from zebrafish (Danio rerio) embryos and it will be applicable to tissues from kidney, liver and mammary gland, which are rich in endogenous biotin or biotinylated proteins (Dakshinamurti and Mistry, 1963;Moss and Lane, 2006;Taniguchi and Watanabe, 2007;Wood and Barden, 1977).…”