Roles of buffer solution and substrate surface on the characteristic of DNA network formed on SiO2

Jo, Young-Sang; Lee, Young-Hun; Roh, Yonghan

doi:10.1016/j.msec.2003.09.154

Cited by 8 publications

(10 citation statements)

References 9 publications

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“…Since, the DNA network formed by first self-assembling MUDA SAMs on gold that was in contact with a DNA assembling solution for 24 h, we know that the DNA network by using AFM is large-scale and uniform. It was also clear that our DNA networks formed in a DNA assembling solution containing 5.5 ng/lL DNA, where DNA networks rarely form by any previously published method (Cai et al, 2000;Jiang and Lin et al, 2004;Jo et al, 2003;Kanno et al, 2000;Oliveira Brett et al, 2003;Tanaka et al, 2001;Wu et al, 2002;Xiao et al, 2003). We therefore concluded that our method is capable of constructing a DNA network when only small amounts of DNA or a low concentration DNA sample are available.…”

Section: Experimental Sectionssupporting

confidence: 53%

“…A large number of DNA networks have been constructed on various substrates including mica (Cai et al, 2000;Kanno et al, 2000;Wu et al, 2002;Xiao et al, 2003), silicon dioxide (Jo et al, 2003), silicon (Tanaka et al, 2001), and highly oriented pyrolytic graphite (Jiang et al, 2004;Oliveira Brett and Chiorcea, 2003). Generally, the DNA networks were fabricated by dropping a solution of high concentration DNA onto a substrate with it remaining there for several minutes, and then blowing it dry with nitrogen or rinsing the drop off with anhydrous ethanol.…”

Section: Introductionmentioning

confidence: 99%

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Large‐scale, Uniform DNA Network on 11‐mercaptoundecanoic Acid Modified Gold (111) Surface: Atomic Force Microscopy Study

Song

Wang

Sun

et al. 2007

Microscopy Res & Technique

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Large-scale, uniform plasmid deoxyribonucleic acid (DNA) network has been successfully constructed on 11-mercaptoundecanoic acid modified gold (111) surface using a self-assembly technique. The effect of DNA concentration on the characteristics of the DNA network was investigated by atomic force microscopy. It was found that the size of meshes and the height of fibers in the DNA network could be controlled by varying the concentration of DNA with a constant time of assembly of 24 h.

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Section: Experimental Sectionssupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Large‐scale, Uniform DNA Network on 11‐mercaptoundecanoic Acid Modified Gold (111) Surface: Atomic Force Microscopy Study

Song

Wang

Sun

et al. 2007

Microscopy Res & Technique

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“…Self‐assembled DNA networks, similar to the one displayed in Figure B, have been reported before (Kanno et al ., ; Wu et al ., ; Xiao et al ., ; Jo et al ., ; Liu et al ., ; Ristic et al ., ). Some of these studies have determined that the substrate, a high density of DNA, and certain ionic buffer conditions are determinants for the formation of networks.…”

Section: Resultsmentioning

confidence: 99%

Robust deposition of lambda DNA on mica for imaging by AFM in air

Cervantes

Gutiérrez–Medina

2014

Scanning

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Long DNA molecules remain difficult to image by atomic force microscopy (AFM) because of their tendency to entanglement and spontaneous formation of networks. We present a comparison of two different DNA deposition methods operating at room temperature and humidity conditions, aimed at reproducible imaging of isolated and relaxed λ DNA conformations by AFM in air. We first demonstrate that a standard deposition procedure, consisting in adsorption of DNA in the presence of divalent cations followed by washing and air-drying steps, yields a coexistence of different types of λ DNA networks with a only a few isolated DNA chains. In contrast, deposition using a spin-coating-based technique results in reproducible coverage of a significant fraction of the substrate area by isolated and relaxed λ DNA molecules, with the added benefit of a reduction in the effect of a residual layer that normally embeds DNA strands and leads to an apparent DNA height closer to the expected value. Furthermore, we show that deposition by spin-coating is also well-suited to visualize DNA-protein complexes. These results indicate that spin-coating is a simple, powerful alternative for reproducible sample preparation for AFM imaging.

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“…DNA networks formed by self-assembly on solid surfaces were generally prepared by facial deposition method using DNA solution with relatively high concentration. The density, height and the coverage of DNA networks were usually regulated as reported by adjusting the concentration of DNA solution, deposition time and the type of substrates including mica (27)(28), silicon (29), gold (30) or other materials (31)(32). In addition, whether the surface was modified or not by salt cations was also a neglectable factor for the fabrication of regular networks (33).…”

Section: Introductionmentioning

confidence: 99%

Controlled Self-Assembly of λ-DNA Networks with the Synergistic Effect of DC Electric Field

Gao

Wang

et al. 2019

Preprint

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Large-scale and morphologically controlled self-assembled λ-DNA networks were successfully constructed by the synergistic effect of DC electric field. The effect of DNA concentration, direction and intensity of the electric field, even the modification of the mica surface using Mg 2+ on the characteristics of the as-prepared DNA networks were investigated in detail by atomic force microscopy (AFM). It was found that the horizontal electric field was more advantageous to the formation of DNA networks with more regular structures. At the same concentration, the height of DNA network was not affected significantly by the intensity change of the horizontal electric field. The modification of Mg 2+ on mica surface increased the aggregation of DNA molecules, which contributed to the morphological change of the DNA networks. Furthermore, DNA molecules were obviously stretched in both horizontal and vertical electric fields at low DNA concentrations. Statement of significanceThrough the synergistic effect of DC electric field, a series of large-scale and morphologically controlled self-assembled λ-DNA networks were successfully fabricated. We found that the horizontal electric field was more advantageous to the formation of DNA networks with more regular structures. At the same concentration of DNA solution, the height of DNA network was not affected significantly by the intensity change of the horizontal electric field. The modification of Mg 2+ on mica surface increased the aggregation of DNA molecules, which contributed to the morphological change of the DNA networks. We suggest this study will promote the understanding on the preparation of controllable self-assembled λ-DNA networks and the application of DNA networks.

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Roles of buffer solution and substrate surface on the characteristic of DNA network formed on SiO2

Cited by 8 publications

References 9 publications

Large‐scale, Uniform DNA Network on 11‐mercaptoundecanoic Acid Modified Gold (111) Surface: Atomic Force Microscopy Study

Large‐scale, Uniform DNA Network on 11‐mercaptoundecanoic Acid Modified Gold (111) Surface: Atomic Force Microscopy Study

Robust deposition of lambda DNA on mica for imaging by AFM in air

Controlled Self-Assembly of λ-DNA Networks with the Synergistic Effect of DC Electric Field

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