Roles of FGF-2 and TGF-beta/FGF-2 on differentiation of human mesenchymal stem cells towards nucleus pulposus-like phenotype

Zhou, Xiaopeng; Tao, Yong; Wang, J; Liang, Chengzhen; H, Li; Chen, Q

doi:10.3109/08977194.2014.969420

Cited by 18 publications

(12 citation statements)

References 40 publications

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“…In relation to chondrogenic differentiation markers, some authors have described the expression of COL2A1 and aggrecan in human adult MSCs differentiated in pellet culture [46]. Our assessment of these markers by Western blot revealed the expression of both proteins in spheroids cultured under normoxia and hypoxia, but greater chondrogenic differentiation was observed in the case of chondrospheroids cultured under hypoxia.…”

Section: Discussionmentioning

confidence: 66%

Adipose-Derived Mesenchymal Stem Cell Chondrospheroids Cultured in Hypoxia and a 3D Porous Chitosan/Chitin Nanocrystal Scaffold as a Platform for Cartilage Tissue Engineering

Zubillaga

Alonso‐Varona

Fernandes

et al. 2020

IJMS

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Articular cartilage degeneration is one of the most common causes of pain and disability in middle-aged and older people. Tissue engineering (TE) has shown great therapeutic promise for this condition. The design of cartilage regeneration constructs must take into account the specific characteristics of the cartilaginous matrix, as well as the avascular nature of cartilage and its cells’ peculiar arrangement in isogenic groups. Keeping these factors in mind, we have designed a 3D porous scaffold based on genipin-crosslinked chitosan/chitin nanocrystals for spheroid chondral differentiation of human adipose tissue-derived mesenchymal stem cells (hASCs) induced in hypoxic conditions. First, we demonstrated that, under low oxygen conditions, the chondrospheroids obtained express cartilage-specific markers including collagen type II (COL2A1) and aggrecan, lacking expression of osteogenic differentiation marker collagen type I (COL1A2). These results were associated with an increased expression of hypoxia-inducible factor 1α, which positively directs COL2A1 and aggrecan expression. Finally, we determined the most suitable chondrogenic differentiation pattern when hASC spheroids were seeded in the 3D porous scaffold under hypoxia and obtained a chondral extracellular matrix with a high sulphated glycosaminoglycan content, which is characteristic of articular cartilage. These findings highlight the potential use of such templates in cartilage tissue engineering.

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Section: Discussionmentioning

confidence: 66%

Adipose-Derived Mesenchymal Stem Cell Chondrospheroids Cultured in Hypoxia and a 3D Porous Chitosan/Chitin Nanocrystal Scaffold as a Platform for Cartilage Tissue Engineering

Zubillaga

Alonso‐Varona

Fernandes

et al. 2020

IJMS

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“…For osteogenic differentiation (51–54), MSC cultures were expanded by treatment with osteogenic induction medium (low‐sugar DMEM supplemented with 10% FBS, 100 nM dexamethasone, 0.2 μM ascorbic acid, and 10 mM β‐glycerophosphate). Cells were maintained by the addition of fresh osteogenic induction medium every 2‐3 d. For chondrogenic differentiation (55), cells were cultured in chondrogenic induction medium [high‐glucose DMEM, 40 μg/ml proline, 100 nM dexamethasone, 50 ng/ml ascorbic acid‐2‐phosphate, ITS‐X (insulin, transferrin, selenium, ethanolamine solution), and 10 ng/ml TGF ‐β 3] for 21 d. Sulfated glycosaminoglycans were stained with Alcian Blue (Cyagen Biosciences, Guangzhou, China).…”

Section: Methodsmentioning

confidence: 99%

IGFBP7 regulates the osteogenic differentiation of bone marrow‐derived mesenchymal stem cellsviaWnt/β‐catenin signaling pathway

Zhang

Chen

et al. 2018

FASEB j.

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Insulin-like growth factor-binding protein 7 (IGFBP7), a low-affinity IGF binder, may play an important role in bone metabolism. However, its function in osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (BMSCs) remains unclear. Therefore, we investigated its effects on osteogenic differentiation. Overexpression of IGFBP7 enhanced the expression of osteo-specific genes and proteins, and IGFBP7 knockdown decreased osteogenesis-specific markers. More mineral deposits and higher alkaline phosphatase activity were observed after the up-regulation of IGFBP7. Moreover, β-catenin levels were up-regulated by the overexpression of IGFBP7 or the addition of extracellular IGFBP7 protein and were reduced by the depletion of IGFBP7. The increase in osteogenic differentiation due to the overexpression of IGFBP7 was partially decreased by specific Wnt/β-catenin signaling inhibitors. Using a rat tibial osteotomy model, a sheet of IGFBP7-overexpressing BMSCs improved bone healing, as demonstrated by imaging, biomechanical, and histologic analyses. Taken together, these findings indicate that IGFBP7 regulates the osteogenic differentiation of BMSCs partly via the Wnt/β-catenin signaling pathway.-Zhang, W., Chen, E., Chen, M., Ye, C., Qi, Y., Ding, Q., Li, H., Xue, D., Gao, X., Pan, Z. IGFBP7 regulates the osteogenic differentiation of bone marrow-derived mesenchymal stem cells via Wnt/β-catenin signaling pathway.

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“…Mesenchymal stem cell (MSC)-based tissue engineering treatment has been widely contemplated for the repair of IVD degeneration and has shown promising perspectives 108,109 . Studies show that TGF-b plays a vital role in it 110,111 . On one hand, TGF-b can synergize with other growth factors to promote the differentiation of MSCs towards NP-like cells, which supplies the quantity of IVD cells for the repair of IVD degeneration 112,113 .…”

Section: The Treatment Prospect Of Tgf-b Signaling In Ivd Degenerationmentioning

confidence: 99%

TGF-β signaling in intervertebral disc health and disease

Chen

Liu

et al. 2019

Osteoarthritis and Cartilage

104

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Objective: This paper aims to provide a comprehensive review of the changing role of transforming growth factor-b (TGF-b) signaling in intervertebral disc (IVD) health and disease. Methods: A comprehensive literature search was performed using PubMed terms 'TGF-b' and 'IVD'. Results: TGF-b signaling is necessary for the development and growth of IVD, and can play a protective role in the restoration of IVD tissues by stimulating matrix synthesis, inhibiting matrix catabolism, inflammatory response and cell loss. However, excessive activation of TGF-b signaling is detrimental to the IVD, and inhibition of the aberrant TGF-b signaling can delay IVD degeneration. Conclusions: Activation of TGF-b signaling has a promising treatment prospect for IVD degeneration, while excessive activation of TGF-b signaling may contribute to the progression of IVD degeneration. Studies aimed at elucidating the changing role of TGF-b signaling in IVD at different pathophysiological stages and its specific molecular mechanisms are needed, and these studies will contribute to safe and effective TGF-b signaling-based treatments for IVD degeneration.

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Roles of FGF-2 and TGF-beta/FGF-2 on differentiation of human mesenchymal stem cells towards nucleus pulposus-like phenotype

Cited by 18 publications

References 40 publications

Adipose-Derived Mesenchymal Stem Cell Chondrospheroids Cultured in Hypoxia and a 3D Porous Chitosan/Chitin Nanocrystal Scaffold as a Platform for Cartilage Tissue Engineering

Adipose-Derived Mesenchymal Stem Cell Chondrospheroids Cultured in Hypoxia and a 3D Porous Chitosan/Chitin Nanocrystal Scaffold as a Platform for Cartilage Tissue Engineering

IGFBP7 regulates the osteogenic differentiation of bone marrow‐derived mesenchymal stem cellsviaWnt/β‐catenin signaling pathway

TGF-β signaling in intervertebral disc health and disease

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