Roles of fibroblasts from the interface zone in invasion, migration, proliferation and apoptosis of gastric adenocarcinoma

Shan, Lihui; Sun, Wenguang; Han, Wei; Qi, Lei; Yang, Chun; Chai, Chee‐Yin; Yao, Ke; Zhou, Qiaogen; Wang, Hongmei; Wang, Lifeng; Liu, Jia-Ren

doi:10.1136/jclinpath-2012-200909

Cited by 21 publications

(14 citation statements)

References 22 publications

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“…Cancer cells attract fibroblasts and fibroblast precursors through secretion of various cytokines and growth factors such as transforming growth factor (TGF)-beta, vascular endothelial growth factor (VEGF), platelet derived growth factor (PDGF) and fibroblast growth factor (FGF) 2 and recruit them into the tumor scaffold [29]. We wanted to study the role of FAP in this context and investigated cell motility within an ECM-like matrix toward a cancer cell driven microenvironment.…”

Section: Discussionmentioning

confidence: 99%

“…Because of its manyfold influence on tumor physiology, restricted expression pattern within diseased areas and correlation with poor clinical outcome in patients with cancer, FAP has emerged as a promising target for cancer therapy [4,5,24,26,27]. Expression of FAP has been connected to poor prognosis in several epithelial tumors [27][28][29]. In particularly aggressive tumor types, such as pancreatic ductal adenocarcinoma (PDAC) and non-small cell lung cancer (NSCLC), FAP has been described as an independent negative prognostic marker.…”

Section: Introductionmentioning

confidence: 99%

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Specific inhibition of fibroblast activation protein (FAP)-alpha prevents tumor progression in vitro

Teichgräber

Monasterio

Chaitanya

et al. 2015

Advances in Medical Sciences

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Specific inhibition of fibroblast activation protein (FAP)-alpha prevents tumor progression in vitro

Teichgräber

Monasterio

Chaitanya

et al. 2015

Advances in Medical Sciences

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“…The TMA slides were stained using the diaminobenzidene HRP Color Development kit (Beyotime Institute of Biotechnology), and then counterstained with hematoxylin (Chemical reagent factory, Guangzhou, China). A negative control was performed without the primary antibodies, and a known IHC-positive slide (Alenabio) was used as a positive control (13,14).…”

Section: Methodsmentioning

confidence: 99%

Reduced expression of sushi domain containing 2 is associated with progression of non-small cell lung cancer

et al. 2015

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Abstract. Sushi domain containing 2 (SUSD2) has been identified as a gene encoding an 822-amino acid protein, which contains a transmembrane domain and functional domains inherent to adhesion molecules. Previous studies have reported that increased expression of SUSD2 has a critical role in tumorigenesis in human breast cancer. However, to the best of our knowledge, SUSD2 expression status and its correlation with the clinicopathological features of non-small cell lung cancer (NSCLC) have not previously been investigated. In the present study, reverse transcription-quantitative polymerase chain reaction and western blotting were used to evaluate SUSD2 messenger RNA (mRNA) and protein expression in NSCLC and adjacent normal lung tissues. The clinicopathological significance of SUSD2 was investigated by immunohistochemical analysis of an NSCLC tissue microarray. Receiver operating characteristic analysis was used to determine the cut-off score for positive expression of SUSD2. Furthermore, the correlation between SUSD2 expression and the clinicopathological features of NSCLC was analyzed by χ 2 test. The results revealed that SUSD2 mRNA (P<0.0001) and protein (P<0.0001) expression levels were significantly decreased in NSCLC tissues compared with those of adjacent normal tissues. When the SUSD2 positive expression percentage was determined to be >47.5% (area under ROC curve, 0.799; P<0.000), positive expression of SUSD2 was observed in 100% (32/32) of normal lung tissues and 55% (88/160) of NSCLC tissues by immunohistochemistry (χ 2 =21.160; P<0.000). Furthermore, it was demonstrated that the reduced SUSD2 protein levels in cancer tissues were positively correlated with poor histological grade (χ 2 =41.764; P<0.000), advanced clinical stage (χ 2 =10.790; P=0.013), higher pT (χ 2 =9.070; P=0.028) and positive regional lymph node metastasis (χ 2 =15.399; P=0.002).In conclusion, these data suggest that the reduced expression of SUSD2 is associated with the progression of NSCLC and may have a role in the pathogenesis of NSCLC.

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“…Immunostaining was done according to our laboratory method. [21] Briefly, the deparaffinised section was quenched with 3% H 2 O 2 for 10 min. After washing with phosphate buffered saline (PBS), the sections were incubated in 5% bovine serum albumin for 20 min, followed by incubation with primary rabbit polyclonal antibody to caveolin-1 (1:50) (Santa Cruz Biotechnology, Inc, USA.)…”

Section: Methodsmentioning

confidence: 99%

Caveolin-1 expression in different types of psoriatic lesions: Analysis of 66 cases

Zhang

Zhou

et al. 2014

Indian J Dermatol

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Background:Caveolin-1 is a key structural and functional protein. Caveolin-1 is known to modulate multiple signal-transducing pathways involved in cell differentiation and proliferation. Psoriasis is viewed as a multifactorial pathology characterized by keratinocyte hyperproliferation and abnormal cell maturation.Objectives:To examine the expression of caveolin-1 in skin biopsies from normal subjects, patients, and subjects with the three respective isoforms of psoriasis (psoriasis vulgaris, localized pustular psoriasis, and erythrodermic psoriasis). The expression level of caveolin-1 was compared among psoriasis vulgaris, localized pustular psoriasis, erythrodermic psoriasis, and normal subjects.Materials and Methods:Using immunohistochemical methods, caveolin-1 protein expression was assayed in four groups. An analysis was conducted on skin samples obtained from 22 normal subjects and 28 patients with psoriasis vulgaris, 22 patients with localized pustular psoriasis, and 16 patients with erythrodermic psoriasis. The statistical analysis of the scoring criteria reflecting the level of Caveolin-1 immunostaining between different groups was determined using the Mann–Whitney U-test.Results:In the normal skin, intense and consistent caveolin-1 staining was present in 22 cases. The Caveolin-1 protein was significantly reduced and showed very weak or absent staining within the tissues of psoriasis vulgaris, localized pustular psoriasis, and erythrodermic psoriasis (respective P < 0.001). Caveolin-1 protein expression in psoriasis vulgaris was higher than that in localized pustular psoriasis and erythrodermic psoriasis (respective P < 0.05). Caveolin-1 protein expression was no different in localized pustular psoriasis and erythrodermic psoriasis (P > 0.05).Conclusion:The finding of this study was consistent with a downregulation of Caveolin-1, which might serve as an etiological factor in the development of psoriasis vulgaris, localized pustular psoriasis, and erythrodermic psoriasis. Further mechanistic investigations are required to prove that Caveolin-1 protein has the potential and may be a novel target for therapy of psoriasis vulgaris, localized pustular psoriasis, and erythrodermic psoriasis.

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Roles of fibroblasts from the interface zone in invasion, migration, proliferation and apoptosis of gastric adenocarcinoma

Abstract: These findings indicate that INFs exhibit a more robust biological modulatory activity than CAFs and NFs. INFs may be a key factor leading to tumour progression and metastasis and may be of use as a tool for post-treatment surveillance.

Cited by 21 publications

References 22 publications

Specific inhibition of fibroblast activation protein (FAP)-alpha prevents tumor progression in vitro

Specific inhibition of fibroblast activation protein (FAP)-alpha prevents tumor progression in vitro

Reduced expression of sushi domain containing 2 is associated with progression of non-small cell lung cancer

Caveolin-1 expression in different types of psoriatic lesions: Analysis of 66 cases

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