Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit

Marco‐Jiménez, Francisco; Borrás, Sara; García‐Domínguez, Ximo; D’Auria, Giuseppe; Vicente, J.S.; Marín, Clara

doi:10.1016/j.theriogenology.2020.09.028

Cited by 10 publications

(8 citation statements)

References 56 publications

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“…This biologically active genus may also help create an extracellular environment in the seminal plasma suitable for sperm activation success [ 76 , 77 ]. Although previous studies have found relationships between microbial community structure and sperm fertilization success [ 78 , 79 ] further work is necessary to determine the function of sperm-associated bacteria.…”

Section: Discussionmentioning

confidence: 99%

Microbiomes of the Sydney Rock Oyster are acquired through both vertical and horizontal transmission

et al. 2022

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Background The term holobiont is widely accepted to describe animal hosts and their associated microorganisms. The genomes of all that the holobiont encompasses, are termed the hologenome and it has been proposed as a unit of selection in evolution. To demonstrate that natural selection acts on the hologenome, a significant portion of the associated microbial genomes should be transferred between generations. Using the Sydney Rock Oyster (Saccostrea glomerata) as a model, we tested if the microbes of this broadcast spawning species could be passed down to the next generation by conducting single parent crosses and tracking the microbiome from parent to offspring and throughout early larval stages using 16S rRNA gene amplicon sequencing. From each cross, we sampled adult tissues (mantle, gill, stomach, gonad, eggs or sperm), larvae (D-veliger, umbo, eyed pediveliger, and spat), and the surrounding environment (water and algae feed) for microbial community analysis. Results We found that each larval stage has a distinct microbiome that is partially influenced by their parental microbiome, particularly the maternal egg microbiome. We also demonstrate the presence of core microbes that are consistent across all families, persist throughout early life stages (from eggs to spat), and are not detected in the microbiomes of the surrounding environment. In addition to the core microbiomes that span all life cycle stages, there is also evidence of environmentally acquired microbial communities, with earlier larval stages (D-veliger and umbo), more influenced by seawater microbiomes, and later larval stages (eyed pediveliger and spat) dominated by microbial members that are specific to oysters and not detected in the surrounding environment. Conclusion Our study characterized the succession of oyster larvae microbiomes from gametes to spat and tracked selected members that persisted across multiple life stages. Overall our findings suggest that both horizontal and vertical transmission routes are possible for the complex microbial communities associated with a broadcast spawning marine invertebrate. We demonstrate that not all members of oyster-associated microbiomes are governed by the same ecological dynamics, which is critical for determining what constitutes a hologenome.

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Section: Discussionmentioning

confidence: 99%

Microbiomes of the Sydney Rock Oyster are acquired through both vertical and horizontal transmission

et al. 2022

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“…To the best of our knowledge, the microbiota in goat buck semen has not yet been studied by these new technologies with very few published papers on other species. Similar to human semen, the dominant phyla in rams, rabbits, and pigs are Proteobacteria and Firmicutes, although the relative amount of these phyla varies (31.2-57.5%), depending on the species [in rams, (9); in rabbits, (10); in pigs, (11,12)]. Actinobacteria phylum is also present in all the species (relative abundance also varies between 3.4 and 22%).…”

Section: Introductionmentioning

confidence: 99%

“…Actinobacteria phylum is also present in all the species (relative abundance also varies between 3.4 and 22%). Bacteroidetes are the first, third, and fourth most abundant phyla in stallions (13), pigs (11), and rabbits (10), respectively. Other reported phyla are Deinococcus-Thermus in rams (9), Fusobacteria in rabbits (10) and stallions (13), and Spirochaetes in stallions (13).…”

Section: Introductionmentioning

confidence: 99%

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Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons

et al. 2022

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Changes in semen microbiota are associated with alterations to sperm quality and fertility. However, the microbiota from most livestock species has not yet been studied. Goats are seasonal breeders, but semen microbiota has never been described in this species, and it is unknown how seasonality affects it. Our study objective is 2-fold: to describe the microbiota in goat buck ejaculates and to determine if it differs between breeding and non-breeding seasons. Semen from six males of the Murciano-Granadina breed was collected during both seasons. Two replicates were performed per male and season on different days. The microbiota was characterized by genomic sequencing technology. Sperm quality was also evaluated. Repetition was not significant for the studied variables. Sperm velocities were higher for the breeding than for the non-breeding season. The ejaculates from both seasons also differed in the proportion of apoptotic spermatozoa. The five dominant phyla were Firmicutes, Proteobacteria, Fusobacteria, Actinobacteria, and Bacteroidetes during the breeding season and Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Cyanobacteria during the non-breeding season. The dominant genus during both seasons was Ureaplasma. Differences in microbial community structure (the beta diversity) were found. A decrease in the relative abundance of the genus Faecalibacterium and an increase in the genera Sphingomonas and Halomonas were observed in the ejaculates collected during the breeding season. Sphingomonas and Faecalibacterium abundance favorably and unfavorably correlated with sperm quality, respectively. In conclusion, the semen microbiota from goat bucks varies between breeding and non-breeding seasons, and the microbiota remains stable for 7 days within a season. In addition, the genera Sphingomonas and Faecalibacterium could be possible biomarkers of semen quality in goat bucks. These results contribute to an in-depth understanding of the effects of reproductive seasonality on goat buck ejaculates.

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“…Увеличение числа нейссерий, клебсиелл и синегнойных палочек и снижение количества лактобацилл было связано с повышенной вязкостью семенной жидкости и олигоастенотератозооспермией (105), следовательно, заболевания, передающиеся половым путем, снижают не только женскую, но и мужскую фертильность. При изучении влияния микробиоты семени на репродуктивный успех у кроликов было установлено, что Lysinibacillus и Flavobacterium могут выступать маркерами потенциальной плодовитости (106).…”

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МИКРОБИОТА И РЕПРОДУКЦИЯ У СЕЛЬСКОХОЗЯЙСТВЕННЫХ ВИДОВ МЛЕКОПИТАЮЩИХ (Обзор)

ПОПОВ¹

2022

S-h. biol.

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Использование специализированных пород животных сельскохозяйственных видов часто сопровождается снижением репродуктивного успеха. В молочном скотоводстве растет количество дней сервис-периода, процедур искусственного осеменения на одну стельность, частоты потерь стельности (С.В. Гуськова с соавт., 2014). Накопленные данные по получению эмбрионов методами in vivo и in vitro и их трансплантации свидетельствуют о значительных (30-60 %) потерях эмбрионов (P.J. Hansen, 2020). Причины низких показателей при применении репродуктивных технологий разнообразны и связаны как с биотическими, так и абиотическими факторами, при этом одним из ключевых при потерях эмбрионов может быть дисбаланс микробных сообществ в отделах репродуктивной системы как самок-доноров, так и самок-реципиентов. Изучение состава микробиоты различных отделов и систем многоклеточного организма в последнее время становится все более доминирующей темой в научной литературе. С появлением современных методов идентификации микробов, например метагеномного секвенирования, выявлено большое микробное разнообразие в разных анатомических отделах макроорганизмов. Накоплены данные о микробном составе и его динамике в органах репродуктивной системы, его связях с воспроизводством у млекопитающих, репродуктивным успехом, протеканием беременности, прогнозированием возможностей возникновения патологических процессов. В работе рассматриваются результаты исследований влияния микробиоты на успешность применения репродуктивных технологий, таких как экстракорпоральное оплодотворение, трансплантация эмбрионов, искусственное осеменение. Обсуждается (F. Marco-Jiménez с соавт., 2020) влияние симбиотических бактерий на фертильность и качество семени. Для млекопитающих это направление малоизучено, и крайне необходимо расширять изучение микробиоты репродуктивного тракта сельскохозяйственных животных. Результаты таких исследований дадут дополнительное понимание репродуктивных процессов и представление о причинах неудачных случаев и о положительных исходах воспроизводства. При этом практическое применение такой информации увеличит шансы успешно применять репродуктивные биотехнологии, снизит затраты, связанные с воспроизводством и терапевтическими вмешательствами при лечении патологий репродуктивной системы, а также откроет возможность для разработки и практического применения новых методов, в частности микробной терапии. Итак, можно сделать вывод, что микробиота органов репродуктивной системы млекопитающих оказывает влияние на физиологические процессы размножения (R. Koedooder с соавт., 2019), и при этом очевидно, что, имея возможность управлять микробными сообществами, человек может повысить шансы наступления репродуктивного успеха при воспроизводстве высокоспециализированных пород сельскохозяйственных животных (P.

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Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit

Cited by 10 publications

References 56 publications

Microbiomes of the Sydney Rock Oyster are acquired through both vertical and horizontal transmission

Microbiomes of the Sydney Rock Oyster are acquired through both vertical and horizontal transmission

Microbiota in Goat Buck Ejaculates Differs Between Breeding and Non-breeding Seasons

МИКРОБИОТА И РЕПРОДУКЦИЯ У СЕЛЬСКОХОЗЯЙСТВЕННЫХ ВИДОВ МЛЕКОПИТАЮЩИХ (Обзор)

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