Roles of Hypoxia in Corpus Luteum Formation

Okuda, Kiyoshi; Nishimura, Ryo

doi:10.1007/978-3-319-43238-0_2

Cited by 3 publications

(6 citation statements)

References 98 publications

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“…Hypoxia was found as an important regulator of steroidogenesis in granulosa and luteal cells [ 10 , 13 , 14 , 15 , 16 , 17 , 18 , 22 , 23 , 24 , 25 , 30 , 36 , 37 ]. As far as hypoxia increased progesterone production in luteinized bovine granulosa cells or murine KK1 cells, it had a negative effect on the biosynthesis of progesterone in the primary bovine luteal cells isolated during the early- and mid-luteal phase [ 10 , 17 , 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…HIF-1 significantly increases in mouse granulosa cells at the time of ovulation and its abundance is regulated by the limited supply of O 2 due to impaired development of the vasculature system as well as stimulatory effects of gonadotropins and growth factors [ 24 ]. Hypoxic conditions enhance progesterone production and expression of vascular endothelial growth factor ( VEGFA ) and STAR in the primary and luteinized bovine granulosa cells, which indicate the essential role of hypoxia and HIF-1 in the secretory function and development of the vasculature system during luteal formation [ 11 , 13 , 14 , 15 , 17 , 18 , 20 , 25 , 26 ]. In contrast, incubation of luteal cells in low O 2 concentrations significantly inhibits basal- and LH/hCG-stimulated progesterone production as well as induces apoptosis [ 14 , 15 , 16 , 17 , 18 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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The Role of Reduced Oxygen Supply and Transcription Factors cJUN and CREB in Progesterone Production during the Corpus Luteum Rescue in Gilts

Przygrodzka

Myszczyński²,

Kłos³

et al. 2022

Animals

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The corpus luteum plays a fundamental role in regulating reproduction via progesterone production. Still, there is little data on factors regulating the maintenance of luteal function during early pregnancy in gilts. Previous studies emphasize the role of hypoxia and HIF-1 in the regulation of steroidogenic and angiogenic genes expression and progesterone production by ovarian cells. Using the corpus luteum of cyclic and early pregnant gilts we analyzed: (1) the in vitro effects of reduced oxygen tension on progesterone production and mRNA expression of HIF1A and luteal function regulators, STAR and VEGFA; (2) the ex vivo profiles of mRNA and protein expression of HIF-1α, STAR, VEGFA and transcription factors-cJUN and CREB, regulating STAR expression, in the corpus luteum of cyclic and pregnant gilts. The synthesis of progesterone was gradually inhibited in cyclic or pregnant gilt luteal tissue (on day 13 of cycle or pregnancy) incubated in a decreasing concentration−20%, 10%, and 3% of oxygen (O2). Luteal tissues of pregnant gilts produced trace amounts of progesterone in 10% O2, which was similar to cyclic gilts in 3% O2. HIF1A expression increased after 24 h of incubation in tissues of cyclic gilts in 3% vs. 20% O2 (p < 0.01), whereas levels of STAR and VEGFA increased significantly in cyclic and pregnant gilt tissues incubated in 10% and 3% vs. 20% O2. The ex vivo mRNA expression of HIF1A and VEGFA was elevated (p < 0.001) on day 14 vs. day 12 of pregnancy. The protein expression of HIF-1 and VEGFA increased (p < 0.001), whereas the level of STAR (mRNA and protein) and progesterone dropped (p < 0.001) on day 14 of the estrous cycle vs. a parallel day of pregnancy and/or day 12 of the estrous cycle. The content of phosphorylated cJUN and CREB was elevated (p < 0.01) in the luteal tissue on day 12 or 14 of pregnancy vs. parallel days of the estrous cycle. These increases of phosphorylated cJUN and CREB may be involved in STAR expression in the luteal tissue during early pregnancy in gilts.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Role of Reduced Oxygen Supply and Transcription Factors cJUN and CREB in Progesterone Production during the Corpus Luteum Rescue in Gilts

Przygrodzka

Myszczyński²,

Kłos³

et al. 2022

Animals

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“…The endothelial cells are involved in the development of new capillaries required for supporting the corpus luteum function through the migration from the preexisting blood vessel [26]. In the corpus luteum establishment, luteal endothelial cells undergo cellular proliferation under the influence of angiogenic factors and hypoxic conditions [67]. The luteal endothelial cells play an essential role as microvascular cells that participate to set up the welldeveloped capillary bed for establishing an appropriate microenvironment for differentiation and maturation of fully functional corpus luteum [65].…”

Section: Luteal Endothelial Cellmentioning

confidence: 99%

The Functions of Reproductive Hormones and Luteal Cell Types of Ovarian Corpus Luteum in Cows

Billhaq¹,

Lee²

2023

Preprint

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The corpus luteum is a temporary reproductive endocrine structure established from a ruptured follicle wall after ovulation. In the ovarian cycle of ruminant species, the corpus luteum organ undergoes a repeated pattern of specific cellular proliferation, differentiation, and transformation. The corpus luteum encounters several physiological events, including growth, function, and regression throughout its life span. Also, the corpus luteum comprises granulosa and theca cells and endothelial cells, such as luteal steroidogenic cells and luteal endothelial cells. These cells play an important role in the physiology of the corpus luteum and the maintenance and degeneration of the corpus luteum. Furthermore, the role of reproductive hormones in the ovaries is important. Representative hormones include estrogen, progesterone, prostaglandin F2α, and oxytocin. Understanding their functions is important in studying the physiological phenomena and various mechanisms of the corpus luteum in the ovary. Therefore, the following review will discuss the role of reproductive hormones and luteal cell types in the microenvironment of the corpus luteum in the bovine ovary.

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“…Low‐level blood flow remains from the preovulatory period to the onset of the late luteal period but when luteal regression occurs blood flow reaches the highest level in ruminants (Cosentino et al, 2018; Wise et al, 1982). Therefore, it is thought that luteal development occurs under hypoxic conditions (Okuda & Nishimura, 2017). Hypoxic conditions stimulate HIF1A expression during luteal development (Berisha et al, 2016, 2017; Boonyaprakob et al, 2005; Yoshioka et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Hypoxic conditions stimulate HIF1A expression during luteal development (Berisha et al, 2016, 2017; Boonyaprakob et al, 2005; Yoshioka et al, 2017). And, HIF1A triggers the expression of angiogenic genes such as VEGF resulting in rapid angiogenesis in luteal tissue (Okuda & Nishimura, 2017). It is emphasized that luteal angiogenesis is essential for the formation of a functional corpus luteum that synthesizes the progesterone needed to maintain a viable pregnancy (Okuda & Nishimura, 2017).…”

Section: Introductionmentioning

confidence: 99%

Differential expression of angiogenesis‐related genes in goat uterus and corpus luteum during pregnancy

Turgut,

Korkmaz Ağaoğlu

2023

Reprod Domestic Animals

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Vascularization and the control of luteal and endometrial development are regulated by hypoxia‐inducible factors (HIFs) and vascular endothelial growth factor (VEGF) during pregnancy. In this study, the mRNA and protein expression levels of HIFs (HIF1A, HIF2A and HIF3A) and VEGF in goat uterine and ovarian tissues during various stages of pregnancy were evaluated. A total of 42 Hair goats were used and were allocated into six groups, namely embryo‐positive (G1), early pregnancy (G2), mid‐term pregnancy (G3), late pregnancy (G4), oocyte‐positive group (G5) and diestrus group (G6). The mRNA expression of the examined genes was evaluated by RT‐qPCR, and protein expression was evaluated by immunohistochemistry (IHC). In caruncles, HIF1A mRNA expression was greater in G1, G2 and G4 than in G3 (p < .05). HIF1A and HIF2A expression was greater in G1 than in G5 (p < .05). In cotyledons, HIF1A, HIF2A and HIF3A mRNA expression was greater in G2 and G3 compared to G4 (p < .05). In luteal tissue, HIF1A mRNA expression was greater in G1 and G2 than in G3 and G4 (p < .05). In the immunohistochemical examination, HIF1A, HIF2A, HIF3A and VEGF immunoreactions were detected in uterine and luteal tissues. Findings suggest that HIFs and VEGF are involved in the regulation of ovarian functions as well as the processes of implantation and placentation.

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Roles of Hypoxia in Corpus Luteum Formation

Cited by 3 publications

References 98 publications

The Role of Reduced Oxygen Supply and Transcription Factors cJUN and CREB in Progesterone Production during the Corpus Luteum Rescue in Gilts

The Role of Reduced Oxygen Supply and Transcription Factors cJUN and CREB in Progesterone Production during the Corpus Luteum Rescue in Gilts

The Functions of Reproductive Hormones and Luteal Cell Types of Ovarian Corpus Luteum in Cows

Differential expression of angiogenesis‐related genes in goat uterus and corpus luteum during pregnancy

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