Roles of lncRNAs Mediating Wnt/β-Catenin Signaling in HCC

Xu, Yating; Yu, Xiao; Sun, Zongzong; He, Yuting; Guo, Wenzhi

doi:10.3389/fonc.2022.831366

Cited by 9 publications

(6 citation statements)

References 176 publications

(189 reference statements)

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“…Additionally, FOXD2-AS1 functioned as a molecular sponge of miR-485-5p to control KLK7 transcription, displaying that the FOXD2-AS1/miR-485-5p/KLK7 axis controlled the progression of Papillary thyroid cancer (PTC) [ 21 ]. In addition, FOXD2-AS1 may also activate Wnt/β-catenin signaling pathway؛ This pathway is active in many different types of tumors and plays an important role in controling the growth of cancer cells [ 22 ]. FOXD2-AS1 regulatory mechanisms are very complex and wide, including activation of LSD1 and EZH2, activating Wnt/β-catenin, TRIB3/Akt and NOTCH signaling pathways, inhibition of downstream gene transcription by competitively binding miRNAs and induction of the epithelial to mesenchymal transition (EMT) [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Upregulation of lnc-FOXD2-AS1, CDC45, and CDK1 in patients with primary non-M3 AML is associated with a worse prognosis

Manoochehrabadi,

Talebi,

Pashaiefar

et al. 2024

Blood Res.

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Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy with an unfavorable outcome. The present research aimed to identify novel biological targets for AML diagnosis and treatment. In this study, we performed an in-silico method to identify antisense RNAs (AS-RNAs) and their related co-expression genes. GSE68172 was selected from the AML database of the Gene Expression Omnibus and compared using the GEO2R tool to find DEGs. Antisense RNAs were selected from all the genes that had significant expression and a survival plot was drawn for them in the GEPIA database, FOXD2-AS1 was chosen for further investigation based on predetermined criteria (logFC ≥|1| and P < 0.05) and its noteworthy association between elevated expression level and a marked reduction in the overall survival (OS) in patients diagnosed with AML. The GEPIA database was utilized to investigate FOXD2-AS1-related co-expression and similar genes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and gene ontology (GO) function analysis of the mentioned gene lists were performed using the DAVID database. The protein–protein interaction (PPI) network was then constructed using the STRING database. Hub genes were screened using Cytoscape software. Pearson correlation analysis was conducted using the GEPIA database to explore the relationship between FOXD2-AS1 and the hub genes. The transcription of the selected coding and non-coding genes, including FOXD2-AS1, CDC45, CDC20, CDK1, and CCNB1, was validated in 150 samples, including 100 primary AML non-M3 blood samples and 50 granulocyte colony stimulating factor (G-CSF)-mobilized healthy donors, using quantitative Real-Time PCR (qRT-PCR). qRT-PCR results displayed significant upregulation of lnc-FOXD2-AS1, CDC45, and CDK1 in primary AML non-M3 blood samples compared to healthy blood samples (P = 0.0032, P = 0.0078, and P = 0.0117, respectively). The expression levels of CDC20 and CCNB1 were not statistically different between the two sets of samples (P = 0.8315 and P = 0.2788, respectively). We identified that AML patients with upregulation of FOXD2-AS1, CDK1, and CDC45 had shorter overall survival (OS) and Relapse-free survival (RFS) compared those with low expression of FOXD2-AS1, CDK1, and CDC45. Furthermore, the receiver operating characteristic (ROC) curve showed the potential biomarkers of lnc -FOXD2-AS1, CDC45, and CDK1 in primary AML non-M3 blood samples. This research proposed that the dysregulation of lnc-FOXD2-AS1, CDC45, and CDK1 can contribute to both disease state and diagnosis as well as treatment. The present study proposes the future evolution of the functional role of lnc-FOXD2-AS1, CDC45, and CDK1 in AML development.

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Section: Discussionmentioning

confidence: 99%

Upregulation of lnc-FOXD2-AS1, CDC45, and CDK1 in patients with primary non-M3 AML is associated with a worse prognosis

Manoochehrabadi,

Talebi,

Pashaiefar

et al. 2024

Blood Res.

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“…In the present study, we identified a ceRNA network of hsa_circ_0004535/hsa-miR-1827/CASP8 may have an important regulatory role in T2DM with NAFLD. Studies in a variety of tumors have found that miR-1827 affects patient prognosis by regulating the expression of downstream target genes through binding to their 3'UTRs [35][36][37] . MiR-1827 by targeting PPAR-δ involved in the T2DM disease process 38 .…”

Section: Discussionmentioning

confidence: 99%

Circ_0004535/miR-1827/CASP8 network involved in type 2 diabetes mellitus with nonalcoholic fatty liver disease

Li,

Zeng,

Tang

et al. 2023

Sci Rep

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Diagnostic delay in type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver disease (NAFLD) patients often leads to a serious public health problem. Understanding the pathophysiological mechanisms of disease will help develop more effective treatments. High-throughput sequencing was used to determine the expression levels of circRNAs, and mRNAs in health controls, T2DM patients, and T2DM with NAFLD patients. Differentially expressed genes (DEcircRs, DEmRs) in T2DM with NAFLD were identified by differential analysis. The miRNAs with targeted relationship with the DEcircRs and DEmRs were respectively predicted to construct a ceRNA regulatory network. In addition, enrichment analysis of DEmRs in the ceRNA network was performed. The expression of important DEcircRs was further validated by quantitative real-time PCR (qRT-PCR). The steatosis was detected in glucose treated LO2 cells by overexpressing circ_0004535, and CASP8. There were 586 DEmRs, and 10 DEcircRs in both T2DM and T2DM with NAFLD patients. Combined with predicted results and differential analysis, the ceRNA networks were constructed. The DEmRs in the ceRNA networks were mainly enriched in Toll-like receptor signaling pathway, and apoptosis. Importantly, dual luciferase experiments validated the targeted binding of hsa_circ_0004535 and hsa-miR-1827 or hsa-miR-1827 and CASP8. qRT-PCR experiments validated that hsa_circ_0004535, and CASP8 was downregulated and hsa-miR-1827 was upregulated expression in peripheral blood of T2DM with NAFLD patients. Abnormal cell morphology, and increased lipid droplet fusion were observed in the glucose treated LO2 cells, overexpression of circ_0004535 and CASP8 ameliorated these changes. Our work provides a deeper understanding of ceRNA mediated pathogenesis of T2DM with NAFLD and provides a novel strategy for treatment.

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“… 94 More information about the role of lncRNAs in mediating WNT/β-catenin in HCC can be found in the review article published recently. 95 …”

Section: Non-coding Rnas Affecting Wnt/β-catenin Signaling In Hccmentioning

confidence: 99%

WNT/β-catenin signaling in hepatocellular carcinoma: The aberrant activation, pathogenic roles, and therapeutic opportunities

Gajos-Michniewicz¹,

Czyż²

2024

Genes & Diseases

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Roles of lncRNAs Mediating Wnt/β-Catenin Signaling in HCC

Cited by 9 publications

References 176 publications

Upregulation of lnc-FOXD2-AS1, CDC45, and CDK1 in patients with primary non-M3 AML is associated with a worse prognosis

Upregulation of lnc-FOXD2-AS1, CDC45, and CDK1 in patients with primary non-M3 AML is associated with a worse prognosis

Circ_0004535/miR-1827/CASP8 network involved in type 2 diabetes mellitus with nonalcoholic fatty liver disease

WNT/β-catenin signaling in hepatocellular carcinoma: The aberrant activation, pathogenic roles, and therapeutic opportunities

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