Roles of Olfactomedin 1 in Muscle and Bone Alterations Induced by Gravity Change in Mice

Shimoide, Takeshi; Kawao, Naoyuki; Morita, Hironobu; Ishida, Masaharu; Takafuji, Yoshimasa; Kaji, Hiroshi

doi:10.1007/s00223-020-00710-6

Cited by 10 publications

(10 citation statements)

References 32 publications

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“…A Western blot analysis was performed with anti‐phosphorylated p38, anti‐p38, anti‐phosphorylated ERK1/2, anti‐ERK1/2, anti‐phosphorylated p65, anti‐p65, anti‐phosphorylated Akt, anti‐Akt, anti‐phosphorylated p70 S6 kinase, anti‐p70 S6 kinase, anti‐PMP22, and anti‐GAPDH antibodies as previously described (Shimoide et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, irisin has potential as a biomarker for the evaluation of exercise and also as a target of drug development for osteoporosis. We recently suggested the potential involvement of several novel myokines, such as follistatin, olfactomedin1 and Dkk2, as well as muscle‐derived extracellular vesicles in muscle wasting and osteopenia induced by mechanical unloading or gravity changes (Kawao, Morita, et al, 2018; Kawao, Morita, et al, 2020; Shimoide et al, 2020; Takafuji et al, 2020, 2021). However, the myokines that play significant roles in the interactions between muscle and bone during exercise remain unknown.…”

Section: Introductionmentioning

confidence: 99%

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Role of peripheral myelin protein 22 in chronic exercise‐induced interactions of muscle and bone in mice

Kawaguchi

Kawao

Muratani

et al. 2022

Journal Cellular Physiology

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Exercise is important for the prevention and treatment of sarcopenia and osteoporosis.Although the interactions between skeletal muscles and bone have recently been reported, the myokines linking muscle to bone during exercise remain unknown. We previously revealed that chronic exercise using treadmill running blunts ovariectomyinduced osteopenia in mice. We herein performed an RNA sequence analysis of the gastrocnemius and soleus muscles of male mice with or without chronic exercise to |

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Role of peripheral myelin protein 22 in chronic exercise‐induced interactions of muscle and bone in mice

Kawaguchi

Kawao

Muratani

et al. 2022

Journal Cellular Physiology

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“…For osteoclast formation in mouse bone marrow cells, cells were collected from tibias of 6 to 8week-old male C57BL/6 J mice. Osteoclast formation was induced in mouse bone marrow cells, as described previously [12]. Mouse bone marrow cells were cultured in αMEM with 10% FBS and 50 ng/ml M-CSF for 3 days.…”

Section: Transfectionmentioning

confidence: 99%

“…Western blot analysis was performed, as described previously [12]. Cells were lysed with radio-immunoprecipitation assay buffer containing protease inhibitor cocktails (Millipore, Bedford, MA, USA) and phosphatase inhibitor cocktails (Wako Pure Chemicals).…”

Section: Western Blotmentioning

confidence: 99%

Influence of Angptl1 on osteoclast formation and osteoblastic phenotype in mouse cells

Ishida

Kawao

Mizukami

et al. 2021

BMC Musculoskelet Disord

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Background Osteoblasts and osteoclasts play important roles during the bone remodeling in the physiological and pathophysiological states. Although angiopoietin family Angiopoietin like proteins (Angptls), including Angptl1, have been reported to be involved in inflammation, lipid metabolism and angiogenesis, the roles of Angptl1 in bone have not been reported so far. Methods We examined the effects of Angptl1 on the osteoblast and osteoclast phenotypes using mouse cells. Results Angptl1 significantly inhibited the osteoclast formation and mRNA levels of tartrate-resistant acid phosphatase and cathepsin K enhanced by receptor activator of nuclear factor κB ligand in RAW 264.7 and mouse bone marrow cells. Moreover, Angptl1 overexpression significantly enhanced Osterix mRNA levels, alkaline phosphatase activity and mineralization induced by bone morphogenetic protein-2 in ST2 cells, although it did not affect the expression of osteogenic genes in MC3T3-E1 and mouse osteoblasts. On the other hand, Angptl1 overexpression significantly reduced the mRNA levels of peroxisome proliferator-activated receptor γ and adipocyte protein-2 as well as the lipid droplet formation induced by adipogenic medium in 3T3-L1 cells. Conclusions The present study first indicated that Angptl1 suppresses and enhances osteoclast formation and osteoblastic differentiation in mouse cells, respectively, although it inhibits adipogenic differentiation of 3T3-L1 cells. These data suggest the possibility that Angptl1 might be physiologically related to bone remodeling.

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“…As for the effects of mechanical stress on the muscle/bone relationships, we previously showed that irisin is involved in mechanical unloading-induced muscle wasting and osteopenia as a myokine linking muscle to bone in mice [ 5 ]. We revealed that follistatin, a physiological myostatin inhibitor, as well as olfactomedin1 are involved in muscle and bone alterations induced by gravity change in mice [ 4 , 7 ]. Moreover, we showed that muscle and serum level of Dickkopf-related protein 2, an inhibitor of Wnt/β-catenin signaling, was positively and negatively regulated by mechanical unloading and hypergravity in mice [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of fluid flow shear stress to mouse muscle cells on the bone actions of muscle cell-derived extracellular vesicless

et al. 2021

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The interactions between skeletal muscle and bone have been recently noted, and muscle-derived humoral factors related to bone metabolism play crucial roles in the muscle/bone relationships. We previously reported that extracellular vesicles from mouse muscle C2C12 cells (Myo-EVs) suppress osteoclast formation in mice. Although mechanical stress is included in extrinsic factors which are important for both muscle and bone, the detailed roles of mechanical stress in the muscle/bone interactions have still remained unknown. In present study, we examined the effects of fluid flow shear stress (FFSS) to C2C12 cells on the physiological actions of muscle cell-derived EV. Applying FFSS to C2C12 cells significantly enhanced muscle cell-derived EV-suppressed osteoclast formation and several osteoclast-related gene levels in mouse bone marrow cells in the presence of receptor activator nuclear factor κB ligand (RANKL). Moreover, FFSS to C2C12 cells significantly enhanced muscle cell-derived EV-suppressed mitochondria biogenesis genes during osteoclast formation with RANKL treatment. In addition, FFSS to C2C12 cells significantly enhanced muscle cell-derived EV-suppressed osteoclast formation and several osteoclast-related gene levels in Raw264.7 cells in the presence of RANKL. Small RNA-seq-analysis showed that FFSS elevated the expression of miR196a-5p and miR155-5p with the suppressive actions of osteoclast formation and low expression in mouse bone cells. On the other hand, muscle cell-derived EVs with or without FFSS to C2C12 cells did not affect the expression of osteogenic genes, alkaline phosphatase activity and mineralization in mouse osteoblasts. In conclusion, we first showed that FFSS to C2C12 cells enhances the suppressive effects of muscle cell-derived EVs on osteoclast formation in mouse cells. Muscle cell-derived EVs might be partly involved in the effects of mechanical stress on the muscle/bone relationships.

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Roles of Olfactomedin 1 in Muscle and Bone Alterations Induced by Gravity Change in Mice

Cited by 10 publications

References 32 publications

Role of peripheral myelin protein 22 in chronic exercise‐induced interactions of muscle and bone in mice

Role of peripheral myelin protein 22 in chronic exercise‐induced interactions of muscle and bone in mice

Influence of Angptl1 on osteoclast formation and osteoblastic phenotype in mouse cells

Effects of fluid flow shear stress to mouse muscle cells on the bone actions of muscle cell-derived extracellular vesicless

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