Rolling circle amplification combined with gold nanoparticles-tag for ultra sensitive and specific quantification of DNA by inductively coupled plasma mass spectrometry

Quantification of rolling circle amplified DNA using magnetic nanobeads and a Blu-ray optical pick-up unit

“…Using an MNB-based assay, we have demonstrated this readout to result in an LOD of DNA coils, which is comparable to those obtained using state-of-the art optical components or bulky magnetic sensing system (Zardán Gomez de la Torre et al, 2011). This LOD can be compatible with clinical needs (Ding et al, 2013;He et al, 2014;Xiang et al, 2013;Lin et al, 2013) if multiple RCA Fig. 4.…”

“…Several groups have proposed approaches based on the use of Au nanoparticles alone (He et al, 2014) or combined with electrochemical (Ding et al, 2013) or surface plasmon resonance (Xiang et al, 2013) readouts for the specific and sensitive detection of DNA. The use of magnetic nanoparticles has recently been Contents lists available at ScienceDirect journal homepage: www.elsevier.com/locate/bios proposed for a simple visual qualitative readout of RCA products (Lin et al, 2013).…”

Nucleic acid quantification using nicking–displacement, rolling circle amplification and bio-bar-code mediated triple-amplification

“…The regression equation was y = 517.043x + 118.288 (y was the ICP-MS intensity with the subtraction of the blank, 10 3 cps; x was the concentration of DNA, fM), and the regression coefficient of the linear curve was R 2 = 0.9933. A detection limit of 3.2 Â 10 À17 M of DNA can be estimated using 3s, which is more sensitive than the previously reported ICP-MS detection with RCA detection [29] and other detection techniques (Table S2 in the Supporting information). The amplification of the present protocol was mainly ascribed to the bio-bar-code technology that not only can efficiently avoid crossreaction but also can load more probes on the AuNPs [30,31] and the triple-amplification of restriction enzyme nicking, RCA reaction and bio-bar-code probes.…”

Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

“…This assay is reported to be specific with a detection limit of 1fM of target DNA. He et al integrated magnetic beads based RCA and gold nanoparticles tags for specific and sensitive SNP detection using inductively coupled plasma mass spectrometry (ICP-MS) detection strategy [90]. This method was reported to detect as low as 0.1fM.…”

“…RCA based ligation is a commonly used LCR technique with detection limit approaching 2 amol for mutant alleles. It has multiplexing and high-throughput capabilities [85][86][87][88]90,91]. It takes 70 min for the whole assay.…”