Nucleic acid quantification using nicking–displacement, rolling circle amplification and bio-bar-code mediated triple-amplification

Applications of Gold Nanoparticles in Non-Optical Biosensors

“…Li et al reported a triple signal amplification strategy based on AuNPs, which combined RCA, nicking displacement and bio-bar-code techniques to perform ultra-sensitive detection of target DNA by ICP-MS. This strategy provided a detection limit as low as 3.2 × 10 −17 M for hepatitis B virus (HBV) DNA [159]. Liu et al reported a novel strategy based on capillary electrophoresis and inductively coupled plasma mass spectrometry (CE-ICP-MS).…”

Tandem blocking of PCR extension to form a single-stranded overhang for facile, visual, and ultrasensitive gene detection

“…As a control, template NF39, which has no X residues, was used. Three primers Pr19, Pr20, and Pr21 (19,20, and 21 nt) containing no X groups were 5 0 -FAM labelled (Table 1). As shown in lanes 2 and 5 in Fig.…”

“…Although single-stranded DNA products have been detected by using DNA-labelled AuNps aer rolling circle amplication (RCA), hybridization chain reaction (HCR), and strand displacement amplication (SDA), these amplication approaches are not comparable with PCR. [19][20][21][22][23] Here we propose a more straightforward methodology in which target gene is visually detected in extremely high sensitivity without using complicated machines. The point is to attach single stranded overhangs onto the end of PCR products by using modied primers.…”

Suppression of rolling circle amplification by nucleotide analogs in circular template for three DNA polymerases

“…Various RCA methods have been widely used in nucleic acids and other molecules detection technology [33][34][35] . By introducing REs recognition sequence, its sensitivity can be increased further.…”