Tandem blocking of PCR extension to form a single-stranded overhang for facile, visual, and ultrasensitive gene detection

Abstract

Here we present a simple but practically useful assay for gene detection. This strategy employs the advantages of both PCR and Au colloidal science, and thus satisfactorily fulfills the factors required for Point-of-Care detection.

Ultrasensitive Electrochemical Detection of cancer-Related Point Mutations Based on Surface-Initiated Three-Dimensionally Self-Assembled DNA Nanostructures from Only Two Palindromic Probes

“…Despite significant advances, these strategies have their own disadvantages, hampering the transition from the test tube to clinical diagnosis. For example, PCR has been considered to be a mature technique for the exponential amplification of nucleotide acids and widely used for the detection of genes. , However, it requires special equipment for accurately controlling temperature cycles and faces the risk of cross contamination associated with the target amplification process. − Similarly, RCA is strongly dependent on a relatively long padlock, and SDA is often limited by the assay ability of the picomolar to subpicomolar level that does not support the direct detection of a low-abundance of target species without sequence pre-amplification . Moreover, single base-mismatched target genes cannot be almost distinguished from the wild one.…”